Skip to main content

Synthesis of some novel 2-(3-cyano -6-(thiophen- 2-yl)-4,4′- bipyridin-2- yloxy)acetohydrazide derivatives: assessment of their cytotoxic activity


A new series of pyrazole, bipyridine, N-amide derivatives and Schiff bases was synthesized using compound 2-(3-cyano-6- (thiophen-2-yl)-4,4′- bipyridin-2-yloxy) acetohydrazide (3) as a starting material. The compounds structures were confirmed depending on the spectroscopic methods and elemental analysis. Also, the compounds were evaluated as anticancer agents by the compounds screened towards adenocarcinoma breast cancer cell line (MCF-7). The compounds showed a promising cytotoxic effect against human breast cancer cells. Compound 7c showed the most effective activity compared to other compounds with (IC50 = 0.6 ± 0.01 μg mL−1) in comparison with the reference drug doxorubicin (IC50 = 1.6 ± 0.02 μg mL−1). While compound 3 is closely active with doxorubicin. Also compounds 2, 4, 6, 7a, 7b and 7d showed noticeable cytotoxic effect. Early and late apoptotic cells were detected using Acridine orange/Ethidium bromide staining technique. The results of biologically screening of the tested compounds give an idea about the importance in the compounds acting against breast cancer and may lead to the discovery of a potent anticancer agent.


Cancer disease is one of the most widely spread diseases nowadays especially breast cancer. Breast cancer comes in various forms either histological or clinical because it is a heterogeneous disease. Its treatment is done through chemotherapy and/or hormone therapy. Heterocyclic compounds that incorporating pyridine moiety appear miscellaneous pharmacological properties such as anticancer [1], antimicrobial [2, 3], anticonvulsant [4], antiviral [5], anti- HIV [6], antifungal and, antibacterial activities [7]. Also the antitumor activity of pyridine ring enhanced by introducing different substituents such as hydrazide bearing either thiazole, thiophene, benzothiophene, triazole or pyrazole, and cyanoacetohydrazide [8]. Studying Structure-activity relationship (SAR) of the compounds is due to the well-reported anticancer activity of these rings. Compounds containing a pyridine group that includes a cyano group have excellent antitumor activity as reported in the previous publications [9,10,11,12,13,14,15]. Based on the reported biological activity of these heterocyclic moieties [16, 17], Schiff bases [18,19,20], triazoles [21, 22], quinolones and spiro compounds [23, 24] as anticancer agents [25] and continuing of my research on the chemistry of the biologically active compounds [25,26,27,28,29,30]. Herein, I designed new biologically active compounds using 2-(6′-(4-chlorophenyl) -3′-cyano-3,4′-bipyridin-2′-yloxy) acetohydrazide(3) as a building block and studying their antitumor activity against breast cancer cell line.

Results discussion


In this research, a one-pot manner was used for the synthesis of compound2-oxo-4-(pyridin-4-yl)-6-(thiophen-2-yl)-1,2-dihydropyridine-3-carbonitrile (1) where all the reaction components, 2-acetylthiophene, 4-pyridine carboxaldehyde, ammonium acetate, and ethyl cyanoacetate were added in the presence of ceric ammonium nitrate (CAN) and then refluxed in ethanol. The resulting compound 1 then alkylated with ethyl bromoacetate in ethanol and in the presence of a catalytic amount of potassium carbonate to give the alkylated derivative ethyl 2-(3-cyano -6-(thiophen-2-yl)-4,4′-bipyridin -2-yloxy)acetate (2). The structure of compound 2 was confirmed depending on the spectral data. For example, in the 1H NMR spectrum, the characteristic signals of the ethoxy group appeared at 1.18 ppm for (CH3) and at 4.15 ppm for (OCH2) and the signal for (NH) group at 8.79 ppm was disappeared. Hydrazionlysis of compound 2 gave the acid hydrazide 3. In the acid hydrazide 1H NMR spectrum the signals of the ethoxy groups at 4.15 and 1.18 ppm were disappeared and new signals appeared at 8.75 and 12. 48 for the (NH-NH2) group. All other signals appeared at their expected position as illustrated in the experimental section. 2-(3-Cyano-6-(thiophen-2-yl)-4,4′- bipyridin-2-yloxy)acetohydrazide (3), is used as a starting matter for the synthesis of all target compounds in this work (Scheme 1).

Scheme 1
scheme 1

Synthesis of 2-(3-cyano-6- (thiophen-2-yl)- 4,4′-bipyridin- 2-yloxy)acetohydrazide (3)

Compound 3 was cyclized into different heterocyclic moieties. Cyclization of 3 with ethyl acetoacetate and/or acetylacetone gave the corresponding. 2-(2-(3- methyl -5-oxo-4,5-dihydropyrazol-1-yl)-2-oxo ethoxy)-6- (thiophen-2-yl)- 4,4′-bipyridine- 3carbonitrile (4) and/or 2-(2-(3,5-dimethyl-1H-pyrazol-1-yl)-2-oxoethoxy)-6- (thiophen-2-yl)-4,4′-bipyridine-3-carbonitrile(5), respectively. The compounds’ structures were confirmed based on their spectroscopic data and their elemental analysis wherein both compounds, the characteristic signals of (NH-NH2) group disappeared. In compound 4 new signals appeared at 1.84 ppm for (CH3) group and at 2.88 for (CH2) in pyrazole ring. While in compound 5 new signals at 1.81, 2.01 for (2CH3) have appeared. Also in the 13C NMR spectra of compound 4 a new signal for the new carbonyl group in pyrazolone ring have appeared. All the appeared signals are in accordance with the expected values. Cyclization of compound 3 with ethyl cyanoacetate or diethyl malonate gave the corresponding 2-(2-(3, 5- dioxopyrazolidin-1-yl)-2-oxoethoxy)-6-(thiophen-2-yl)-4,4′-bipyridine-3-carbonitrile (6) (Scheme 2). In the 1H NMR spectrum of compound 6 a characteristic signal of (CH2) at 2.51 ppm in pyrazolidine ring have appeared.

Scheme 2
scheme 2

Synthesis of pyrazole derivatives (4-6). Reagents and conditions :(i) ethyl acetoacetate/AcOH,reflux;(ii) diethyl malonate/AcOH or ethyl cyanoacetate/AcOH, reflux; (iii) acetylacetone/AcOH reflux

A new series of expectedly biologically active N-amide derivatives and Schiff bases was synthesized. Schiff bases 7ad were obtained through condensation of compound 3 with different aldehydes namely 3-pyridine carboxaldehyde, 3,4- Diydroxy benzaldehyde, anisaldehyde and vanillin, in acetic acid. In all Schiff bases the signal characteristic to the (NH2) group was disappeared and the signal of (NH) group at 12.48 ppm was shifted to new positions at 12.48, 9.99, 12.49 and 8.84 ppm. In compounds 7a, 7b, 7c, and 7d respectively. All the characteristic signals of the arylidine groups were appeared at their expected positions as shown in the experimental part, Compound 7c structure was confirmed based on the spectroscopic data in (Fig. 1) The reaction of 3 with p-toluenesulfonyl chloride in absolute ethanol afforded the corresponding 2-(3-cyano-6(thiophen-2-yl)-4,4″-bipyridin-2-yloxy)N-(tosylmethylene)aceto hydrazide (8) (Scheme 3). Compound 8 structure was confirmed based on the spectroscopic data and the elemental analysis.

Fig. 1
figure 1

Analysis for the compound 7C: a infrared spectrum, b nuclear magnetic resonance spectrum, c mass spectrum

Scheme 3
scheme 3

Synthesis of Schiff base 7ad and compound 8

In vitro anticancer screening

The in vitro cytotoxic activities of compounds 1, 2, 3, 5, 6, 7ad and 8 were determined using SRB assay towards breast cancer cell line (MCF-7) over concentration range of 0.01 to 1000 μg. The tested compounds exhibited a variable cytotoxicity profile against the tested human breast cancer cells. (Table 1 and Fig. 2). doxorubicin is a reference drug in this study The IC50: is the compounds concentrations reduce the cell viability to 50%. The data in Table 1 and Fig. 2 indicate the cytotoxicity profile of the newly synthesized compounds against breast cancer cells. The results showed considerable cytotoxicity against cancer cell, most of the compounds showed highly cell killing significant on MCF-7 cells; some of them were revealed a strong activity, others were found to be on par near the reference drug toxicity (IC50 =  1.3 μg mL−1).

Table 1 The IC50 (µg mL−1) of the compounds 1, 2, 3, 5, 6, 7a–d, 8 against breast cancer (MCF-7) cell line
Fig. 2
figure 2

The dose response curves of the cytotoxicity of different compounds towards MCF-7tumor cell line. Cells were exposed to plant extract with different concentrations for 72 h. Cell viability was determined by SRB stain

The Schiff base 7c (IC50 =  0.6 μg mL−1) is the most potent compound in this evaluation and it showed higher activity than doxorubicin itself; then the sulphonamide derivative 3 with (IC50   =  1.3 μg mL−1) have activity nearly to the reference drug. Compounds 1, 3, 6, 7a, 7b, and 7d had a highly toxic effect against breast cancer cell with IC50s ranging from 1.3 to 4.7 μg mL−1 compared to doxorubicin, and the compound 2 has a moderate cytotoxic effect with IC50 = 8.2 μg mL−1. While compound 5 has a weak activity with IC50 = 28.7 μg mL−1 compared with other compounds and compared to doxorubicin.

After staining cells using double stains AO/EtBr, cells appeared in the form of four colors as follows: living cells (normal green nuclei), early-programmed cell death (apoptotic) (bright green nucleus with segmented chromatin), late-programmed cell death (apoptotic) (orange nucleus with chromatin condensation or fragmentation) and necrotic cells (Kernel of uniformly colored orange cells).

The uniformly stained green cells with normal, round and intact nuclei that indicates the healthy cell control. Whereas, the highly cell killing with late apoptotic observed by treatment with compound 1 and some necrotic cell also observed with the compound itself; on the other hand there are no necrotic cells with compounds 2 and 3 compared to compound 1, and the derivative acetohydrazide 3 have high rate of late apoptotic compared to compounds 1 and 2 (Figs. 3 and 4).

Fig. 3
figure 3

Nuclear morphological conersions of MCF-7 cells, after treated using chemical compounds 1, 2, and 3 compared with reference drug doxorubicin. Compounds stimulate different nuclear changes such as condensation and fragmentation of chromatin, nuclei condensation, as demonstrated by acridine orange/Ethidium bromide staining at 200×. Yellow arrows indicate live cell, pink arrows indicate early apoptotic Red arrows indicate necrotic and blue arrows indicate late apoptotic cells

Fig. 4
figure 4

Rate of apoptotic MCF-7 tumor cells after 48 h treatment with chemical compounds (mean ± SD of three independent experiments in three repeats each) compared to cell control

The compound 5 killing the cells with early apoptotic way was more pronounced compared to compounds 3 and 6. Compound 6 has a necrotic cells after treatment compared to compounds 3 and 5. Also, compound 3 have cells with late apoptotic more than compounds 5 and 6 (Figs. 5 and 6).

Fig. 5
figure 5

Morphological and nuclear changes of MCF-7, tumor cells after treatment by chemical compounds 3, 5, and 6 compared with reference drug doxorubcin. Compounds induced various nuclear features such as chromatin fragmented and condensation, nuclei condensation, as demonstrated by acridine orange/Ethidium bromide staining at 200 × . Yellow arrows indicate live cell, pink arrows indicate early apoptotic Red arrows indicate necrotic and blue arrows indicate late apoptotic cells

Fig. 6
figure 6

Rate of apoptotic MCF-7 tumor cells after 48 h treatment with chemical compounds (mean ± SD of three independent experiments in three repeats each) compared to cell control

Compound 7d has a highly late apoptotic effect on cancer cells compared to 3, 7a, 7b, 7d and compound 7c then 3 have early apoptotic more than 7a, 7b, and 7d is lower (Figs. 7 and 8).

Fig. 7
figure 7

Morphological and nuclear changes of MCF-7, tumor cells after treatment by chemical compounds 3, 7a, 7b, 7c and 7d compared with reference drug doxorubcin. Compounds induced various nuclear changes such as chromatin fragmented and condensation, nuclei condensation, as demonstrated by acridine orange/Ethidium bromide staining at 200×. Yellow arrows indicate live cell, pink arrows indicate early apoptotic Red arrows indicate necrotic and blue arrows indicate late apoptotic cells

Fig. 8
figure 8

Rate of apoptotic MCF-7 tumor cells after 48 h treatment with chemical compounds (mean ± SD of three independent experiments in three repeats each) compared to cell control

Whereas, compound 8 has early apoptotic killing effect and cell necrotic against cancer cells more than compound 3, while compound 3 has a more cell late apoptotic effect than compound 8 (Figs. 9 and 10).

Fig. 9
figure 9

Morphological and nuclear changes of MCF-7, tumor cells after treatment by chemical compounds 3, and 8 compared with reference drug doxorubcin. Compounds stimulate different nuclear changes such as chromatin fragmented and condensation, nuclei condensation, as demonstrated by acridine orange/Ethidium bromide staining at 200×. Yellow arrows indicate live cell, pink arrows indicate early apoptotic Red arrows indicate necrotic and blue arrows indicate late apoptotic cells

Fig. 10
figure 10

Rate of apoptotic MCF-7 tumor cells after 48 h treatment with chemical compounds (mean ± SD of three independent experiments in three repeats each) compared to cell control

The biological activity of the tested compounds were indicated the promising cell killing effect of the 4,4′ bipyridine moiety in the compounds acting towards breast tumor cells.


In this paper I used compound 2-(3-cyano-6-(thiophen-2-yl)-4,4′-bipyridin -2-yloxy)acetohydrazide 3 to synthesis a novel substituted pyrazole, bipyridine, N-amide derivatives and Schiff bases. The anticancer activity of the compounds was assessed against breast cancer cell line (MCF-7). The data obtained for the tested compounds shows the possible importance of these compounds to act as anticancer agents where compound 7c showed better activity than the standard drug itself. While other compounds such as compound 3 is equipotent with the standard drug. Compounds 2, 4, 6, 7a, 7b and 7d showed obvious activities but less than the reference.

Materials and methods


Melting points were measured on a Gallenkamp apparatus, and are uncorrected. The desired time for completing the reaction was monitored by TLC. The IR spectra were recorded using (KBR) plates on a Shimadzu 470 IR spectrometer. The 1H and 13C NMR spectra were measured on a Bruker 400DRX-Avance NMR spectrometer at 400 MHz and chemical shifts (δ) are in ppm relative to TMS (tetramethylsilane). Mass spectra were measured on GC/MS with electron impact ionization by to (70 eV). Elemental analyses were performed on Perkin-Elmer 2400 series П CHN elemental analyser.

Synthesis of 2-oxo-4-(pyridin-4-yl) -6-(thiophen-2-yl) -1,2- dihydro pyridine-3-carbonitrile (1)

4-Pyridine carboxaldehyde (0.01 mol), 2-acetyl thiophene (0.01 mol), ethyl cyanoacetate (0.01 mol), ammonium acetate (0.15 mol) and 5 mol% of CAN in ethanol (25 mL) in a 50 mL round-bottom flask were refluxed for 2 h. After completion of the reaction, the solid product obtained was collected, filtered, washed several times by water dried and then crystallized from ethanol to give compound 1 as yellow crystals in yield 89%, m.p. 205 °C. IR (KBr): 3093 (NH), 2218 (CN), 1673 (C = O) cm−1. 1H NMR (DMSO-d6) δ: 7.24–8.67 (m, 8H, Ar–H, thiophene and pyridine rings), 8.79 (s, 1H, NH) ppm. 13C NMR (DMSO-d6) δ: 163.53 (C =O), 162.81, 150.71 (2C), 143.52 (2CH), 142.89, 142.32 (2C), 131.68, 129.59, 129.01 (3CH), 122.50 (2CH), 121.9 (C), 116.14 (CN), 113.38 (CH) ppm. MS: m/z (%): 279 (M + , 20), 224 (100). Anal. Calc. (%) for C15H9N3OS: C, 64.50; H, 3.25; N, 15.04; S, 11.48. Found: C, 64.55; H, 3.18; N, 15.11; S, 11.45.

Synthesis of ethyl 2-(3-cyano-6-(thiophen-2-yl)-4,4′-bipyridin-2-yloxy) acetate (2)

A mixture of compound 1 (0.01 mol), ethyl bromoacetate (0.01 mol), and anhydrous potassium carbonate (0.15 mol) in acetone was refluxed for 2 h. After completion of the reaction the mixture was poured onto the ice, the product separated was collected by filtration, dried, and crystallized from ethanol to give 2 as pale yellow needles in yield 75%, m.p. 159–160 °C. IR (KBr): 2224 (CN), 1753 (C =O), 1600 (C=N) cm−1. 1H NMR (DMSO-d6) δ: 1.18 (t, 3H, J = 6.8, CH3), 4.15 (q, 2H, J=6.8, OCH2), 5.06 (s, 2H, CH2), 7.24-8.82 (m, 8H, Ar–H, thiophene and pyridine rings) ppm. 13C NMR (DMSO-d6) δ: 163.26(C = O), 153.48 (C), 150.71 (C), 143.38 (2CH), 142.38 (C), 132.59, 129.88, 129.64 (3CH), 123.51 (2CH), 114.95 (CN), 113.14 (CH), 91.57 (2C), 64.25 (OCH2), 61.29 (CH2), 14.65 (CH3) ppm. MS: m/z (%): 365 (M + , 100). Anal. Calc. (%) for C19H15N3O3S. C, 62.45; H, 4.14; N, 11.50; S, 8.77. Found: C, 62.48; H, 4.19; N, 11.45; S, 8.68.

Synthesis of 2-(3-cyano-6-(thiophen-2-yl) -4,4′-bipyridin-2-yloxy) aceto hydrazide (3)

A mixture of hydrazine hydrate (99%, 0.04 mol), and compound 2 (0.01 mol), was refluxed in 20 mL absolute ethanol for 5 h. The reaction mixture was poured on an ice-water. The product formed was filtered of, washed with water, dried, and crystallized from ethanol to give 3 as yellow crystals in yield 65%, m.p 226 °C. IR (KBr): 3402.43, 3334.92 (NH2), 3267 (NH), 2212 (CN), 1741 (C =O), 1620 (C=N) cm−1. 1H NMR (DMSO-d6) δ: 4.67 (s, 2H, CH2), 7.18–8.58 (m, 8H, Ar–H, thiophene and pyridine rings), 8.75 (d, 2H, NH2), 12.48(s, 1H, NH) ppm. 13C NMR (DMSO-d6) δ: 160.82 (C =O), 153.20, 153.16 (2C),150.53 (2CH), 143.38 (2C), 143.10(C), 131.25, 129.45, 129.19 (3CH) 123.47 (2CH), 116.33 (CN), 111.44 (CH), 101.86 (CH), 85.76 (C), 56.50 (OCH2) ppm. MS (m/z, %): 351 (M + , 20), 101 (100). Anal. Calc. (%) for C17H13N5O2S. C, 58.11; H, 3.73; N, 19.93; S, 9.12. Found: C, 58.17; H, 3.68; N, 19.96; S, 9.18.

General procedure for the synthesis of compounds 4-6

An equimolar amount of ethyl acetoacetate, acetylacetone and/or ethyl cyanoacetate (or diethyl malonate) and a mixture of compound 3 (0.01 mol) was refluxed in 15 mL acetic acid for 5 h. The produced product after cooling was filtered off, washed with water, dried, and crystallized with acetic acid to give compounds 4, 5, and 6 respectively.

2-(2-(3-methyl-5-oxo-4,5-dihydropyrazol-1-yl)-2-oxoethoxy)-6-(thiophen-2-yl)-4,4′-bipyridine-3 carbonitrile (4)

Pale yellow crystals in yield 71%, m.p. 202–204 ℃. IR (KBr):2347.37 (CN), 1670.35 (C =O),1637.56(C =O),1620 (C=N)cm−1. 1H NMR (DMSO-d6) δ: 1.84 (s, 3H, CH3), 2.88 (s, 2H, CH2), 4.67(s, 2H, CH2), 8.78–7.19 (m, 8H, Ar–H, thiophene and pyridine rings) ppm. 13C NMR (DMSO-d6) δ: 169.17 (C =O), 167.57 (C=O), 150.38 (C), 147.72 (C), 124.10 (2CH), 144.94 (2CH), 144.78 (C), 129.45, 128.97, 127.44 (3CH), 124.10 (CH), 111.44 (CN), 101.86 (CH), 58.16 (OCH2), 42.59 (CH2), 22.88 (CH3) ppm. MS: m/z (%): 417.03 [M + , 17], 293 (100).Anal.Calc.(%) for C21H15N5O3S.C, 60.42; H, 3.62; N, 16.78; S, 7.68. Found C, 60.47; H, 3.68; N, 16.83; S, 7.65,

2-(2-(3,5-dimethyl-1H-pyrazol-1-yl)-2-oxoethoxy)-6-(thiophen-2-yl)-4,4′-bipyridine-3-carbonitrile (5)

Pale yellow crystals in yield 50%, m.p. 197–198 °C.IR (KBr): 3265 (NH), 2213 (CN), 1745 (C =O), 1619 (C=N) cm−1. 1H NMR (DMSO-d6) δ: 1.81 (s, 3H, CH3), 2.01 (s, 3H, CH3), 4.66 (s, 2H, CH2), 6.21-8.75 (m, 9H, CH pyrazole, pyridine and thiophene rings) ppm. 13C NMR (DMSO-d6) δ: 162.02 (C =O), 147.78 (C), 145.72 (C), 144.94 (2CH), 144.50 (C) 143.39(2CH), 128.54, 128.06, 127.45 (3CH), 123.85 (CH), 111.44 (CN), 101.86 (CH), 57.37 (OCH2), 15.31 (CH3) ppm. MS: m/z (%): 415 [M + , 7], 293 (100). Anal.Calc. (%) for C22H17N5O2S.;C,63.60;H,4.12;N,16.86;S,7.72. Found C 63.65; H 4.18; N 16.83; S, 7.77.

2-(2-(3,5-dioxopyrazolidin-1-yl)-2-oxoethoxy)-6-(thiophen-2-yl)-4,4′-bipyridine-3-carbonitrile (6)

Pale yellow crystals in yield 69%, m.p 216–217 °C. IR (KBr): 3400 (NH), 2223 (CN), 1718 (C = O), 1701(C = O), 1617(C=N) cm−1.1H NMR (DMSO-d6) δ: 2.51 (s, 2H, CH2) pyrazoldine) 4.66 (s, 2H, CH2), 7.21-8.77 (m, 8H, Ar–H, thiophene and pyridine rings), 10.10 (s, 1H, NH) ppm. 13C NMR (DMSO-d6) δ: 175.18 (C=O), 170.05 (2C=O), 153.18 (C =N), 150.38 (C), 144.60 (2CH), 143 (C), 138.34 (C), 129.09, 128.98, 127.44 (3CH), 113.50 (CN), 111.41 (CH), 101.81 (CH), 52.56 (OCH2), 22.65 (CH2) ppm. MS: m/z (%): 419 [M + , 20], 292 (100). Anal.Calc.(%) for C20H13N5O4S.C, 57.28; H, 3.12; N, 16.70; S, 7.664. Found C, 57.33; H, 3.16; N, 16.75; S, 7.69.

General procedure for synthesis of Schiff bases 7a-d

A mixture of compound 3 (0.01 mol) and the appropriate aromatic aldehyde (3-pyridincarboxaldehyde, 3, 4 -dihydroxy benzaldehyde, anisaldehyde and vanillin (0.01 mol) in the presence of a catalytic amount of pipredine, in absolute ethanol (10 mL) was refluxed for 2 h. After cooling, the formed precipitate was filtered off, dried, and crystallized from acetic acid to afford the corresponding Schiff base 7a-d.

(E)-2-(3-cyano-6-(thiophen-2-yl)-4,4′-bipyridin-2-yloxy)-N’-(pyridin-3-ylmethylene)acetohydrazide (7a)

Pale Yellow crystals in yield 75%, m. p 189–190 °C. IR (KBr): 3337 (NH), 2347.37 (CN), 1655 (C =O), 1595 (C =N) cm−1. 1H NMR (DMSO-d6) δ: 4.66 (s, 2H, CH2), 8.7–7.18 (m, 13H, Ar–H, thiophene, pyridine and CH alphatic), 12.48 (s, 1H, NH) ppm. 13C NMR (DMSO): δ = 162.03 (C=O), 153.16 (C = N), 151.56 (C) 150.38 (C), 149.67 (C), 147.73 (CH), 144.95 (CH), 144.77 (C), 143.10 (CH), 129.46, 128.97, 127.45 (3CH), 124.10 (3CH), 111.44 (CN), 101.87 (CH), 54.99 (OCH2) ppm.MS: m/z (%): 440[M + , 20], 374 (100).Anal.Calc. (%)for C23H16N6O2S. C, 62.72; H, 3.66; N, 19.08; S, 7.28. Found C,62.76; H,3.70; N,19.11; S, 7.32.

(E)-2-(3-cyano-6-(thiophen-2-yl)-4,4′-bipyridin-2-yloxy)-N’-(3,4-dihydroxybenzylidene)acetohydrazide (7b)

Brown crystals in yield 71%, m.p.198–199 °C.IR (KBr):  3400 (OH), 3179(NH), 2347 (CN), 1655, (C =O), 1612 (C =N) cm−1. 1H NMR (DMSO-d6) δ: 4.67 (s, 2H, CH2), 7.19–8.78 (m, 12H, Ar–H, thiophene, pyridine and CH aliphatic), 9.99 (s, 1H, NH), 12.49 (br. s, 1H, OH), 13.71 (br. s, 1H, OH) ppm. 13C NMR (DMSO): δ = 169.99 (C=O), 153.17 (C=N), 152.91 (C), 152.03 (C), 151.56 (C), 149.79(2CH), 144.50 (CH), 143.10 (C), 143.10 (CH), 129.11, 128.97, 128.10 (3CH), 124.10 (3CH), 111.44 (CN), 101.86 (CH), 52.57 (OCH2) ppm. MS: m/z (%): 471 [M + ,19], 293 (100). Anal.Calc. (%) for C24H17N5O4S.C, 61.14; H, 3.63; N, 14.85; S, 6.80.Found C, 61.18;H, 3.67; N, 14.89; S, 6.84.

(E)-2-(3-cyano-6-(thiophen-2-yl)-4,4′-bipyridin-2-yloxy)-N’-(4-methox ybenzyli dene) cetohydrazide (7c)

Pale Yellow crystals in yield 85%, m.p180–181 °C. IR (KBr): 3348 (NH), 2218 (CN), 1630 (C = O), 1580 (C = N) cm−1. 1H NMR (DMSO-d6) δ: 3.83(s, 3H, CH3), 4.67 (s, 2CH, CH2), 8.88–7.03 (m, 13H, Ar–H, thiophene, pyridine and CH aliphatic), 12.49 (s, 1H, NH) ppm. 13C NMR (DMSO): δ = 162.74 (C=O), 153.63 (C=N), 153.17 (C), 152.23 (C), 151.56 (C), 149.58 (2CH), 144.22 (CH), 143.27 (C), 143.10 (CH), 128.97, 128.06, 127.44 (3CH), 123.37 (2CH), 111.44 (CN), 107.84 (CH), 101.87 (CH), 55.91 (OCH2), 47.13 (OCH3) ppm. MS: m/z (%): 469 [M + , 27], 462 (100). Anal. Calc. (%) for C25H19N5O3S. C, 63.95; H, 4.08; N, 14.92; S, 6.83.Found C, 63.91; H, 4.11; N, 14.96; S, 6.87.

(E)-2-(3-cyano-6-(thiophen-2-yl)-4,4′-bipyridin-2-yloxy)-N’-(4-hydroxy-3 methoxy benzylidene) acetohydrazide (7d)

Pale Yellow crystals in yield 80%, m.p.240–241 °C. IR (KBr): 3402.43 (OH), 3339 (NH), 2222 (CN), 1654.92 (C =O), 1618 (C =N) cm−1. 1H NMR (DMSO - d6) δ: 1.49 (s, 3H, CH3), 4.67 (s, 2H, CH2), 8.78 -6.88 (m, 12H, Ar–H, thiophene, pyridine and CH aliphatic), 8.84 (s, 1H, NH), 12.58 (br. s, 1H, OH) ppm. 13C NMR (DMSO):δ = 160.33 (C=O), 153.60 (C=N), 153.17 (C), 152.16 (C), 151.82 (C), 151.56 (C), 149.56 (2CH), 148.68 (C), 147.72 (CH), 144.78 (C), 144.42 (CH), 129.10, 128.97, 127.99 (3CH), 123.38 (2CH), 115.80 (CH), 113.53 (CH), 111.44 (CN), 106.31 (CH), 101.86 (C), 55.81 (OCH2), 44.04 (OCH3) ppm. MS: m/z (%): 485 [M + , 14], 306.76 (100).Anal.Calc. (%)forC25H19N5O4S.C, 61.85; H, 3.94; N, 14.42; S 6.60 found C 61.88; H 3.89;N 14.45; S 6.56.

Synthesis of (E)-2-(3-cyano-6(thiophen -2-yl)-4,4″- bipyridin-2-yloxy) N-(tosylmethylene)acetohydrazide (8)

A mixture of p-toluenesulfonyl chloride (1 mmol) and compound 3 (1 mmol) and in 10 mL of absolute ethanol was refluxed for 3 h. The formed precipitate was filtered, washed with water, dried, and recrystallized from dioxane to give buff crystals in yield 60%, m.p 279 – 280 °C. IR (KBr): 3350 (NH), 2200 (CN), 1700 (C = O), 1645 (C = N), 1620 (C = N) cm−1. 1H NMR (DMSO - d6) δ: 1.05 (s, 3H, CH3), 4.08 (s, 2H, CH2), 8.26–7.12 (m, 13H, Ar–H, thiophene, pyridine and CH aliphatic), 8.96 (s, 1H, NH), 12.58 (s, 1H, NH) ppm. 13C NMR (DMSO): δ = 163.09 (C=O), 152.50 (C=N), 145.93 (C), 145.54 (CH), 144.41 (C), 143.15 (CH), 138.24 (C), 130.13 (C), 129.10 (CH), 128.13, 127.88, 127.47 (3CH), 125.96 (2CH), 112.49 (CN), 101.86 (CH), 56.49 (OCH2), 21.24 (CH3) ppm. MS: m/z (%): 518 [M + , 15], 262 (100).Anal. Calc.(%) for C25H19N5O4S2.C,58.01; H, 3.70;N, 13.53; S, 12.39. Found C,58.07;H, 3.67; N, 13.55; S, 12.34.

In vitro cytotoxic activity

Cell culture

Breast carcinoma (MCF-7) human cell line was obtained from the American type culture collection (ATCC). Cells were maintained in RPMI-1640 supplemented with (100 μg/mL); penicillin (100 units/mL) and heat-inactivated fetal bovine serum (10% v/v) in a humidified, 5% (v/v) CO2 atmosphere at 37° [31, 32].

Cytotoxicity assay

The cytotoxicity of the chemical compounds was evaluated against (MCF-7) human tumor cell using Sulphorhodamine B assay (SRB) in King Khalid University, biology department. 80% confluency growing cells were trypsinized and cultured in a 96 well tissue culture plate for 24 h before treatment with the chemical compounds. Cells were exposed to the six different concentrations of each compound (0.01, 0.1, 1, 10, and 1000 µg/ml); untreated cells (control) were added. The cells were incubated with the concentrations for 72 h and subsequently fixed with TCA (10% w/v) for 1 h at 4 °C. After several washings, cells were stained by 0.4% (w/v) SRB solution for 10 min in dark place. Excess stain was washed with 1% (v/v) glacial acetic acid. After drying overnight, the SRB-stained cells were dissolved with Tris–HCl and the color intensity was measured in microplate reader at 540 nm. The relation between viability percentage of each tumor cell line and compounds concentrations was analyzed to get the IC50 (dose of the drug which reduces survival to 50%) using Sigma Plot 12.0 software [33].

Acridine orange/ethidium bromide staining for detection of early and late apoptotic cells

DNA binding dyes Acridine orange (AO) and Ethidium bromide (EtBr), were used for the morphological detection of viable, apoptotic and necrotic cells. AO is taken up by both non-viable and viable cells that emit green fluorescence when intercalated into DNA. EtBr is taken up only by nonviable cells whereas; it is excluded by viable cells and emits red fluorescence by intercalation into DNA. Cells were seeded on cover slide inside six well plates. Cells were incubated in CO2 incubator with 37 °C temperature and 5% CO2 for 24 h then treated with IC50s concentration of the chemical compounds and incubated for 48 h. Cells were washed with cold PBS 1× for three times. Cells were stained with a mixture Acridine Orange 100 μg/ml/Ethidium Bromide (AO/EB) 100 μg/ml in PBS 1x with 10% FBS on each well and then incubated for 5 min in RT. The cover slides with cultured stained cells were transfer immediately to new slides and the cells were ready to be visualized by the blue filter of the fluorescence microscope [34, 35].

Availability of data and materials

All the data supporting findings are contained within the manuscript.



Breast cancer cell line


Structure–activity relationship


Ceric ammonium nitrate


Nuclear magnetic resonance spectroscopy


Sulforhodamine B

IC50 :

The half maximal inhibitory concentration (IC50)


Acridine Orange Ethidium Bromide


Thin-layer chromatography

The IR spectra:

Infrared spectroscopy


Potassium bromide




Gas chromatography–mass spectrometry

13C NMR:

Carbon-13 Nuclear Magnetic Resonance Spectroscopy


Dimethyl Sulfoxide


American type culture collection


Sulphorhodamine B assay


Acridine orange


Ethidium bromide


Fetal bovine serum


Phosphate-Buffered Saline


  1. Bernardino AM, de Azevedo AR, da Silva Pinheiro LC, Borges JC, Carvalho VL, Miranda MD, de Meneses MD, Nascimento M, Ferreira D, Rebello MA, Da Silva VA, de Frugulhetti ICPP (2007) Synthesis and antiviral activity of new 4-(phenylamino)/4-[(methylpyridin-2-yl) amino]-1-phenyl-1H-pyrazolo [3, 4-b] pyridine-4-carboxylic acids derivatives. Med Chem Res. 16:352

    Article  CAS  Google Scholar 

  2. Radwan MAA, Alshubramy MA, Abdel-Motaal M, Hemdan BA, El-Kady DS (2020) Synthesis, molecular docking and antimicrobial activity of new fused pyrimidine and pyridine derivatives. Bioorg Chem 96:103516.

    Article  CAS  PubMed  Google Scholar 

  3. Patel NB, Agravat SN (2009) Synthesis and antimicrobial studies of new pyridine derivatives. Chem Heterocycl Compd 45:1343–1353

    Article  CAS  Google Scholar 

  4. Paronikyan EG, Noravyan AS, Dzhagatspany IA, Nazaryan IM, Paronikyan RG (2002) Synthesis and anticonvulsant activity of isothiazolo [5, 4-b] pyrano (thiopyrano)[4, 3-d] pyridine and isothiazolo [4,5-b]-2, 7-naphthyridine derivatives. Pharm Chem J 36:465

    Article  CAS  Google Scholar 

  5. Kong S, Zhang J, Li X, Pan H, Guo D (2020) de novo biosynthesis of indole-3-ethanol and indole-3-ethanol acetate in engineered Escherichia coli. Biochem Eng J 154:107432

    Article  Google Scholar 

  6. Kumar S, Gupta Sh, Abadi LF, Gaikwad Sh, Desai D, Bhutani KK, Kulkarni S, Singh IP (2019) Synthesis and invitro anti–HIV–1 evaluation of novel pyrazolo[4,3–c] pyridin–4–one derivatives. Eur J Med Chem 183:111714.

    Article  CAS  PubMed  Google Scholar 

  7. Eswaran S, Adhikari AV, Pal NK, Chowdhury IH (2010) Design and synthesis of some new quinoline-3-carbohydrazone derivatives as potential antimycobacterial agents. Bioorg Med Chem Lett. 20(3):1040–1044

    Article  CAS  Google Scholar 

  8. Al-Said MS, Bashandy MS, Al-Qasoumi SI, Ghorab MM (2011) Anti-breast cancer activity of some novel 1, 2-dihydropyridine, thiophene and thiazole derivatives. Eur J Med Chem. 46:137

    Article  CAS  Google Scholar 

  9. El-Zahar MI, Abd El-karim SS, Haiba ME (2009) Synthesis and cytotoxic evaluation of some novel 6-(benzofuran-2-yl)-4-(4-fluorophenyl) pyridines. W J Chem. 4:182

    CAS  Google Scholar 

  10. Azzam RA, Elgemeie GH, Osman RR (2020) Synthesis of novel pyrido [2, 1-b] benzothiazole and N-substituted 2-pyridylbenzothiazole derivatives showing remarkable fluorescence and biological activities. J Mol Struct. 1201:127194.

    Article  CAS  Google Scholar 

  11. Badawi AM, El-Sharkawy H, Ismail DA (2008) Synthesis, characterization, and antitumor activity of four novel sulphonamide compounds. Aust J Basic Appl Sci 2:301

    Google Scholar 

  12. Pannala M, Kher S, Wilson N, Gaudette J, Sircar I, Zhang SH, Bakhirev A, Yang G, Yuen P, Gorcsan F, Sakurai N (2007) Synthesis and structure–activity relationship of 4-(2-aryl-cyclopropylamino)-quinoline-3-carbonitriles as EGFR tyrosine kinase inhibitors. Bio org Med Chem. 17:5978

    Article  CAS  Google Scholar 

  13. Frost BM, Lonnerholm G, Nygren P, Larsson R, Lind Hagen E (2002) In vitro activity of the novel cytotoxic agent CHS 828 in childhood acute leukemia. Anticancer Drugs 13:735

    Article  CAS  Google Scholar 

  14. French FA, Blanz EJ Jr, Shaddix SC, Brockman RW (1974) alpha-(N)-Formylheteroaromatic thiosemicarbazones. Inhibition of tumor-derived ribonucleoside diphosphate reductase and correlation with in vivo antitumor activity. J Med Chem. 17:172

    Article  CAS  Google Scholar 

  15. Al-Shareef HF, Elhady HA, Aboellil AH, Hussein EM (2016) Ammonium chloride catalyzed synthesis of novel Schiff bases from spiro [indoline-3, 4′-pyran]-3′-carbonitriles and evaluation of their antimicrobial and anti-breast cancer activities. SpringerPlus. 5:887

    Article  Google Scholar 

  16. Bouabdallah I, M’Barek LA, Zyad A, Ramdani A, Zidane I, Melhaoui A (2006) Anticancer effect of three pyrazole derivatives. Nat Prod Res 20:1024

    Article  CAS  Google Scholar 

  17. Abadi AH, Eissa AA, Hassan GS (2003) Synthesis of novel 1,3,4-trisubstituted pyrazole derivatives and their evaluation as antitumor and antiangiogenic agents. Chem Pharm Bull 51:838

    Article  CAS  Google Scholar 

  18. Sriram D, Yogeeswari P, Kumar TA (2005) Microwave assisted synthesis, AntiHIV, and AntiYFV activities of schiff bases of N-HY-Droxy-N1-Aminoguanidine tosylate. Indian J Pharm Sci. 67:493–496

    CAS  Google Scholar 

  19. Avanish K, Rajesh K (2011) Areview on synthesis of Schiff, bases of 2-amino- 4-phenyl thiazole. Int Res J Pharm 2(6):11–12

    CAS  Google Scholar 

  20. Abd El-Latif NA, Amr AE, Ibrahiem AA (2007) Synthesis, reactions, and 10 a natural synthon. Monatsh Chem 138:559

    Article  CAS  Google Scholar 

  21. Bonte J (2000) Third generation aromatase inhibitors and inactivators in the treatment and prevention of breast cancer. Eur J Cancer 36:114

    Article  Google Scholar 

  22. Roberts K, Rickett K, Greer R, Woodward N (2017) Management of aromatase inhibitor induced musculoskeletal symptoms in postmenopausal early Breast cancer: a systematic review and meta-analysis. Crit Rev Oncol Hematol. 111:66–80.

    Article  PubMed  Google Scholar 

  23. Elhady HA, Al-Shareef HF (2019) Design, synthesis, anti-proliferative evaluation and cell cycle analysis of hybrid 2-quinolones. Anti Cancer Agents Med Chem (Formerly Current Medicinal Chemistry-Anti-Cancer Agents). 19:1132–1140

    Google Scholar 

  24. Ahmed SA, Khairou KS, Asghar BH, Muathen HA, Nahas NM, Alshareef HF (2014) Photochromism of tetrahydroindolizines. Part XIV: synthesis of cis-fixed conjugated photochromic pyridazinopyrrolo [1, 2-b] isoquinolines incorporating carbon-rich linkers. Tetrahedron Lett. 55:2190–2196

    Article  CAS  Google Scholar 

  25. Abdel-Mohsen SA, Hussein EM (2014) A green synthetic approach to the synthesis of Schiff bases from 4-amino-2-thioxo-1, 3-diazaspiro [5.5] undec-4-ene-5-carbonitrile as potential anti-inflammatory agents. Russ J Bioorg Chem. 40:343–349

    Article  CAS  Google Scholar 

  26. Elhady HA, Mohamed SM, Al-Shareef HF, El-Mekawy RE (2019) Synthesis, reactions, and applications of 2-thiohydantoin derivatives. Acta Poloniae Pharm. 76(6):971–986

    Google Scholar 

  27. Abdelaal MY, Sobahi TR, Al-Shareef HF (2013) Modification of chitosan derivatives of environmental and biological interest: a green chemistry approach. Int J Biol Macromol. 55:231–239

    Article  CAS  Google Scholar 

  28. Hussein E, AlShareef HF, Aboellil AH, Elhady HA (2015) synthesis of some novel 6′-(4-chlorophenyl)-3,4′-bipyridine-3′-carbonitriles: assessment of their antimicrobial and cytotoxic activity. Z Naturforsch 70(11):783

    Article  CAS  Google Scholar 

  29. Alshareef HF, Mohamed HA, Salaheldin A (2017) Synthesis and biological evaluation of new tacrine analogues under microwave irradiation. Chem Pharm Bull 65:732–738

    Article  CAS  Google Scholar 

  30. Abdelaal MY, Aboellil AH, Sobahi TR, Al-Shareef HF (2016) Mutual effect of chitosan derivatives and some microbes on the microbial activity. Int Res J Nat Appl Sci 3(6):200–208

    Google Scholar 

  31. Mahmoud AM, Al-Abd AM, Lightfoot DA, El-Shemy HA (2012) Anti-cancer characteristics of mevinolin against three different solid tumor cell lines was not solely p53-dependent. J Enzyme Inhib Med Chem. 27:673–679

    Article  CAS  Google Scholar 

  32. Ibrahim SR, Abdallah HM, Mohamed GA, Ross SA (2016) Integracides HJ: new tetracyclic triterpenoids from the endophytic fungus Fusarium sp. Fitoterapia. 112:161–167

    Article  CAS  Google Scholar 

  33. Alahdal AM, Asfour HZ, Ahmed SA, Noor SA, Al-Abd AM, Elfaky MA, Elhady SS (2018) Anti-helicobacter, antitubercular and cytotoxic activities of scalaranes from the Red Sea sponge hyrtios erectus. Molecules 23(4):978.

    Article  CAS  PubMed Central  Google Scholar 

  34. Liu EH, Qi LW, Wu Q, Peng YB, Li P (2009) Anticancer agents derived from natural products. Mini Rev Med Chem 9:1547–1555.

    Article  CAS  PubMed  Google Scholar 

  35. Albright F, Stephenson RA, Agarwal N, Teerlink CC, Lowrance WT, Farnham JM, Albright LA (2015) Prostate cancer risk prediction based on complete prostate cancer family history. J Korean Med Sci 75:390–398.

    Article  Google Scholar 

Download references


The author thank Dr. Heba Elhady Mohamed for her helpful discussion and review of this work.


This research received no external funding.

Author information

Authors and Affiliations



All work was performed by the sole author. The author read and approved the final manuscript.

Corresponding author

Correspondence to Hossa F. Al Shareef.

Ethics declarations

Competing interests

No competing of interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Al Shareef, H.F. Synthesis of some novel 2-(3-cyano -6-(thiophen- 2-yl)-4,4′- bipyridin-2- yloxy)acetohydrazide derivatives: assessment of their cytotoxic activity. BMC Chemistry 14, 40 (2020).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: