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In vitro antioxidant properties of the biflavonoid agathisflavone

Chemistry Central Journalvolume 12, Article number: 75 (2018) | Download Citation

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Abstract

Purpose

Free radicals are considered as the causative agents of a variety of acute and chronic pathologies. Natural antioxidants have drawn attention of the researchers in recent years for their ability to scavenge free radicals with minimal or even no side effects. This study evaluates the antioxidant capacity of agathisflavone, a naturally occurring biflavonoid by a number of in vitro methods.

Methods

Agathisflavone was subjected to DPPH, ABTS, OH and NO radical scavenging assay, reducing potential and inhibition of lipid peroxidation (TBARS) test using trolox as a standard.

Results

Agathisflavone showed concentration-dependent antioxidant activity against all types of free radicals used in this study. The antioxidant capacity, reducing potential and inhibition of lipid peroxidation showed by agathisflavone were comparable to that of trolox.

Conclusion

Agathisflavone exhibited antioxidant capacity, which suggests considering this biflavonoid for the use in the prevention and/or treatment of diseases precipitated by oxidative stress.

Introduction

Free radicals, both reactive oxygen and nitrogen species (ROS/RNS), produced by partial reduction of oxygen and nitrogen respectively, are inevitable processes of our body [1,2,3]. Some of these ROS and RNS are involved in various fundamental processes of cell survival, including mitochondrial respiration and regulation, signalization, cell proliferation and differentiation. However, any alteration in the cellular levels of the ROS/RNS can induce oxidative stress leading to cellular damages, hampering proper cellular functioning or even cell death [4, 5]. Prolonged exposure to ROS/RNS results in the damages to cell macromolecules such as carbohydrates, proteins, lipids as well as genetic materials (e.g., DNA, RNA) which eventually gives rise to the pathophysiology of a number of serious diseases including cancer, diabetes, atherosclerosis, immunosuppression, swelling, cardiovascular diseases and neurodegenerative disorders [6, 7]. Evidence suggests that certain neurological disorders such as, Alzheimer’s disease, Parkinson’s disease, schizophrenia, anxiety and depression have a direct link to long term exposure of the cells to excessive oxidative stresses [8]. In this context, naturally occurring antioxidants have been studied extensively to find their possible role in the prevention and cure for such diseases [9, 10].

Flavonoids represent an important class of natural antioxidants with significant therapeutic potential. Biflavanoids, a type of flavonoids, comprehends a large group of compounds with promising effect against oxidative damage [11].

The biflavonoids are structurally dimer of flavonoids connected with each other by a C–C or C–O glycosidic linkage [12]. Same as those flavonoids, bioflavonoids have been reported for a number of biological activities proved through in vitro and ex vivo studies, which include anti-inflammatory [13,14,15], inhibition of cytochrome P450 enzymes [16], antiviral [17, 18], and neuroprotective activity [19, 20]. Agathisflavone, chemically known as (8-[5,7-dihydroxy-2-(4-hydroxyphenyl)-4-oxochromen-6-yl]-5,7-dihydroxy-2-(4-hydroxyphenyl)chromen-4-one) is a yellow color biflavonoid with the molar mass of 538.457 g/mol, density 1.656 g/cm3 and splitting coefficient 5.09 (Fig. 1) [21]. It occurs in different parts (e.g. leaf, stem, fruit, root) of many plants including Caesalpinia pyramidalis, Anacardium occidentale, Rhus parviflora and can be extracted by polar or medium polar solvents including methanol and ethanol [22,23,24,25]. Previous studies suggest that agathisflavone has some important biological activities, including antiviral [26], antimicrobial [27], and neuroprotective [28] activity. However, little research was done with the intent of describing the antioxidant property of this compound.

Fig. 1
figure1

Chemical structure of agathisflavone

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Thus the present study was designed to assess the antioxidant property of this biflavonoid by evaluating its ability to scavenge the free radicals, namely DPPH, ABTS, hydroxyl and nitric oxide (NO). It was also tested for its capacity to inhibit lipid peroxidation by TBARS method and the ability to transfer electron by ferric reducing assay.

Materials and methods

Chemicals

Trolox, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,20-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), thiobarbituric acid (TBA), trichloroacetic acid (TCA), sodium nitroprusside (SNP), 2,20-azobis-2-amidinopropane dihydrochloride (AAPH), 2-deoxyribose and potassium ferricyanide were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). All chemicals and solvents used were of analytical grade.

Plant

Fresh leaves of Caesalpinia pyramidalis Tull. (Family: Fabaceae) was collected from Valente, Brazil and identified by Prof. Dr. L. P. de Queiróz (State University of Feira de Santana) and Prof. Dr. M. de F. Agra (Laboratory of Pharmaceutical Technology UFPB). A voucher specimen was deposited at the herbarium of Alexandre Leal da Costa of Biology Institute of the Federal University of Bahia (accession number 240291).

Extraction, isolation and identification of agathisflavone

Fresh leaves of C. pyramidalis was extracted with methanol and chromatographed using silica gel to get pure agathiflavone. The structure was elucidated by spectroscopic data including 1D, 2D NMR and LS–MS carried out at the Department of Chemistry, Federal University of Bahia, Brazil (data not shown). Noteworthy to mention here that naturally occurring agathisflavone exhibits atropisomerism and thus was isolated as a mixture of the atropisomers.

In vitro antioxidant activity evaluation

DPPH radical scavenging test

This test was done according to the method described by Machado et al. [29] with slight modifications. Briefly, a reaction mixture containing of 0.5 mL of agathisflavone (0.058, 0.116, 0.232, 0.464 and 0.928 mM) was mixed with 1.4 mL of DPPH stock solution in ethanol (100 µM). Resulting solution was mixed vigorously and kept in the dark at room temperature for 30 min. Same procedure was followed for trolox used as standard and the absorbance was measured for both agathisflavone or trolox against control at 517 nm. The percentage of inhibition was calculated by the following equation:

$$ \% \;Inhibition\;of\;DPPH = \left( {\frac{{A_{c} - A_{t} }}{{A_{c} }}} \right) \times 100 $$

where Ac and At is the absorbance of the control and test (agathisflavone/trolox), respectively.

ABTS radical scavenging test

The method described by Re et al. [30] with slight modifications was used for this test. The ABTS radical cation was initially formed by mixing 5 mL of 7 mM ABTS with 88 µL of 2.45 mM of potassium persulfate (K2S2O8) solution with further incubation at room temperature in the absence of light for 16 h. The resulting solution was diluted in ethanol in such a way to obtain an absorbance of 0.70 ± 0.05 at 734 nm. This test was done in the dark and at room temperature. An aliquot (0.5 mL) of agathisflavone/trolox (0.058–0.928 mM) solution was mixed with 1.96 mL of the ABTS solution and the absorbance was measured after 6 min. The results were expressed as percentage of inhibition of the ABTS in a similar fashion to that of DPPH radical scavenging assay.

OH radical scavenging assay

Hydroxyl radical (OH·) was generated by Fenton reaction [31] with slight modifications. Different concentrations (0.058–0.928 mM) of agathisflavone/trolox at was added to the reaction medium containing FeSO4 (6 mM), 2-deoxyribose (5 mM), H2O2 (100 mM) and phosphate buffer (20 mM, pH 7.4). After incubating the mixture for 30 min at ambient temperature, the reaction was terminated by the addition of phosphoric acid (4%, w/w), followed by the addition of 1% TBA (50 mM, NaOH aqueous solution). It was then heated for 15 min at 95 °C, cooled and the absorbance was measured at 734 nm in a spectrophotometer. The results were expressed as percentage of 2-deoxyribose degradation.

$$ \% \;2{\text{-}}Dexyribose\; degradation\; = \left( {\frac{{A_{c} - A_{t} }}{{A_{c} }}} \right) \times 100 $$

where Ac and At is the absorbance of the control and test (agathisflavone/trolox), respectively.

Reducing potential assay

The method described by Singhal et al. [32] with slight modifications was used to determine the reducing capacity of agathisflavone. Briefly, 1 mL of agathisflavone/trolox (0.058–0.928 mM) was added with 1 mL of 1% potassium ferricyanide and 0.5 mL of sodium phosphate buffer (0.2 M, pH 6.6). The reaction mixture was incubated at 50 °C for 20 min, followed by the addition of 0.5 mL of 10% TCA, 0.5 mL of distilled water and 0.25 mL of 0.1% ferric chloride. The absorbance of the reaction mixture was measured at 700 nm in a spectrophotometer against a blank solution. The EC50 of the sample and standard required for 50% reduction potential was determined.

NO scavenging test

Nitric oxide was produced from the spontaneous decomposition of sodium nitroprusside in 20 mM phosphate buffer (pH 7.4). Once generated, nitric oxide interacts with oxygen to produce nitrite ions, which was measured by the Griess reaction [33]. The reaction mixture containing 1 mL of SNP in phosphate buffer (20 mM) and 0.5 mL of agathisflavone/trolox (0.058–0.928 mM) was incubated at 37 °C for 1 h. An aliquot (0.5 mL) of the reaction mixture was taken and homogenized with 0.5 mL of Griess reagent. Absorbance of chromophore was measured at 540 nm in a spectrophotometer and the results were expressed as percentage inhibition of nitrite ions.

Inhibition of lipid peroxidation (TBARS test)

The TBARS (thiobarbituric acid reactive substances) method described by Guimarães et al. [34] was used evaluate the ability of agathisflavone to inhibit lipid peroxidation. Briefly, an aliquot of (0.5 mL) of egg yolk homogenate (5% w/v in 50 mM phosphate buffer, pH 7.4) was mixed with 0.5 mL of agathisflavone/trolox (0.058–0.928 mM). The lipid peroxidation was induced by the addition of 0.5 mL of AAPH (200 mM) for 60 min at 37 °C. Subsequently, 1 mL of TCA (10%) and 1 mL of TBA (0.67%) was added and heated at 97 °C for 15 min. After 15 min, the reaction mixture was centrifuged and the absorbance of the supernatant was measured at 532 nm. The extent of lipid peroxidation was estimated by TBARS levels formed and the results were expressed as percentage of inhibition of lipid peroxidation:

$$ \% \;Inhibition\;of\;lipid\;peroxidation = \left( {\frac{{A_{c} - A_{t} }}{{A_{c} }}} \right) \times 100 $$

where Ac and At is the absorbance of the control and test (agathisflavone/trolox), respectively.

Statistical analysis

All the experiments were done in triplicate and the results are expressed as mean ± standard error of mean (SEM). Statistical analysis was performed using the program GraphPad Prism® 6.02 (San Diego, CA, USA), one-way ANOVA, multiple comparisons following by t-Student–Newman–Keuls post hoc test. The results were considered statistically significant at p < 0.05.

Results

DPPH radical scavenging

Agathisflavone was found to scavenge DPPH radical in a concentration-dependent manner with the highest inhibition observed for the highest concentration of agathisflavone tested (0.928 mM). The radical scavenging capacity of agathisflavone was almost similar to that of the trolox at all concentrations (except at 0.232 and 0.464 mM). The EC50s calculated for agathisflavone and trolox were 0.474 mM (0.399–0.564 mM) and 0.149 mM (0.129–0.173 mM), respectively at 95% confidence interval (Fig. 2).

Fig. 2
figure2

DPPH· scavenging capacity of agathisflavone and trolox. [Values are mean ± SEM (n = 3) ap < 0.05 when compared to the system (100% of DPPH·), bp < 0.05 when compared to the trolox (standard) (ANOVA and t-Student–Neuman–Keuls as a post hoc test)]

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ABTS scavenging assay

Concentration dependent ABTS radical scavenging activity was observed for both agathisflavone and trolox with the highest inhibition observed for the highest concentration tested (Fig. 3). EC50s calculated for agathisflavone and trolox were 0.179 mM (0.137–0.234 mM) and 0.311 mM (0.283–0.341 0.474 mM), respectively with 95% confidence interval.

Fig. 3
figure3

ABTS radical scavenging capacity of agathisflavone and trolox. [Values are mean ± SEM (n = 3); ap < 0.05 when compared to the system (100% of ABTS), bp < 0.05 when compared to trolox (ANOVA and t-Student–Neuman–Keuls as a post hoc test)]

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OH radical scavenging assay

In this study, agathisflavone showed a concentration-dependent OH radical scavenging capacity. The activity was slightly lower than that of trolox only at 0.116 and 0.232 mM, but higher at other concentrations with statistical significance (p < 0.05) (Fig. 4). EC50s calculated for agathisflavone and standard were 0.163 mM (0.101–0.263 mM) and 0.372 mM (0.280–0.496 mM), respectively with 95% confidence interval.

Fig. 4
figure4

OH· scavenging capacity of agathisflavone and trolox. [Values are mean ± SEM (n = 3) ap < 0.05 when compared to the system (100% of OH·), bp < 0.05 when compared to the trolox (standard) (ANOVA and t-Student–Neuman–Keuls as a post hoc test)]

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Reducing potential

Both agathisflavone and trolox reduced ferric ion to ferrous in a concentration dependent manner (Fig. 5). The activity was slightly higher for trolox than that of agathisflavone. The EC50s calculated for the sample and standard were 0.163 mM (0.101–0.263 mM) and 0.372 mM (0.280–0.496 mM), respectively with 95% confidence interval.

Fig. 5
figure5

Reducing potential (Fe3+/Fe2+) of agathisflavone and trolox. [Values are mean ± SEM (n = 3) ap < 0.05 when compared to the system (without agathisflavone/trolox), bp < 0.05 when compared to the trolox (standard) (ANOVA and t-Student–Neuman–Keuls as a post hoc test)]

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NO scavenging assay

Agathisflavone exhibited prominent NO scavenging activity which was evident from the reduced production of nitrite ion (Fig. 6). Concentration-dependant antioxidant NO scavenging capacity was observed for both agathisflavone and standard and the activity was slightly higher for agathisflavone that that of trolox. EC50s calculated for the sample and standard were 0.209 mM (0.162–0.2682 mM) and 0.456 mM (0.415–0.493 mM), respectively with 95% confidence interval.

Fig. 6
figure6

NO radical scavenging capacity of agathisflavone and trolox. [Values are mean ± SEM (n = 3) ap < 0.05 when compared to the system (100% of nitrite ions), bp < 0.05 when compared to the trolox (standard) (ANOVA and t-Student–Neuman–Keuls as a post hoc test)]

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TBARS test

In this test, agathisflavone was found to be a better inhibitor of lipid peroxidation that that of trolox at all the concentrations tested (Fig. 7). Both agathisflavone and trolox showed concentration-dependent inhibition of lipid peroxidation evident from the reduced levels of EC50s calculated for agathisflavone and standard were 0.179 mM (0.154–0.208 mM) and 0.352 mM (0.233–0.530 mM), respectively with 95% confidence interval.

Fig. 7
figure7

TBARS inhibitory capacity of agathisflavone and trolox. [Values are mean ± SEM (n = 3) ap < 0.05 when compared to the system (100% of TBARS levels), bp < 0.05 when compared to trolox (standard) (ANOVA and t-Student–Neuman–Keuls as a post hoc test)]

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Discussion

Present investigation suggests that agathisflavone possesses prominent antioxidant capacity as observed from several in vitro antioxidant test systems. There are four hydroxyl groups in the structure of agathisflavone (Fig. 8a), i.e., 4′–OH, 7–OH, 7″–OH and 4‴–OH, (Fig. 8b) that can donate H· (radical hydrogen) to reduce free radicals. Although the rest two hydroxyls (5″–OH and 5–OH) radicals cannot donate radical hydrogen, but they do participate in the process by forming resonance structures (Fig. 8c, d).

Fig. 8
figure8

Proposed mechanism of antioxidant activity of the agathisflavone by donating of H· and electrons. a Chemical structure of agathisflavone (OH groups at various positions). b Possible C-terminals from where proton (H+) can be donated. c and d Proton (H+) donation and neutralization of free radicals, resonance structures of agathisflavone

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Antioxidant activity of several flavonoids with mechanism similar to that of agathisflavone have been studied using methods as DPPH and/or ABTS, OH and NO scavenging assay, as well as their ability to inhibit lipid peroxidation by TBARS assay and reducing ability [35, 36].

When compared, agathisflavone was found to be a better scavenger of ABTS than that of DPPH. Although both ABTS and DPPH are free radicals, but the differ in the way that DPPH is a stable free radical itself but ABTS is formed instantly in the reaction solution. Thus both of the methods are used for antioxidant activity study but a difference in the EC50 of a compound can occur due to the difference in the mechanism of action of neutralizing free radicals. In relating the results of DPPH· (EC50 = 0.895 mM) and ABTS·+ (EC50 = 0.123 mM) tests, it is possible to say that agathisflavone showed a better scavenging capacity of ABTS·+. This may be due to effects of donation of electrons, leading to the reduction of ABTS·+ formation. On the other hand, transference of hydrogen atoms may occur to form of a DPPH stable molecule by the action of this biflavonoid [37, 38]. Our findings are similar to the results suggested by Ye et al. [39], who were isolated a biflavonoid from the methanolic extract of the Camellia oleifera Abel shells and with a chemical structure that of the agathisflavone.

Agathiflavone isolated from the leaf extract of Anacardium occidentale was subjected to DPPH radical scavenging assay by Ajileye et al. [25]. The EC50 value calculated for agathisflavone was 366.37 μg/mL, which is equivalent to 0.679 mM, slightly lower that the value observed in the present work (0.474 mM). This slight change may happen due to the variation of the DPPH· concentration in the reaction mixture.

The compounds that have large quantities of ·OH (free) in their chemical structures have higher reducing potential [11]. In a previous study the antioxidant activity of the natural biflavonoid (morelloflavone-4000-O-b-d-glicosil, fukugiside e morelloflavone) isolated from the ethyl acetate extract of the dried fruits of Garcinia brasiliensis was seen with the potential reduction capacity [9]. Like the evaluated compound in this study, the biflavonoids isolated by Gontijo et al. [9] showed reducing capacities in a concentration-dependent manner.

The ·OH oxidizing radical and its presence in the reaction medium promotes degradation of 2-deoxyribose. In our study, agathisflavone at all concentration tested may react with ·OH, thus the inhibition of the degradation of the monosaccharide utilized [3, 40]. Based on the comparison between the EC50 values of the agathisflavone and the antioxidant standard (trolox) in inhibiting the 2-deoxyribose degradation, this biflavonoid can be considered as a potent scavenger of ·OH. This may be an indication of protecting important biomolecules, such as proteins, lipids and genetic materials (e.g.—DNA, RNA) [41].

Agathisflavone also significantly (p < 0.05) inhibited the levels of NO. An excessive generation of NO is related to a number of pathological conditions, including intracellular oxidative damages and cell death [13, 41]. Thus, the inhibitory effects of this damaging radicals may inhibit or protect cells and cellular organelles from the damaging effects of NO. Furthermore, peroxyl radicals generated by AAPH are evident to cause lipid peroxidation [42]. In our study, we found that the agathisflavone significantly inhibited TBARS production in comparison to the NC and trolox, suggesting a prominent protective capacity of the lipid molecules from oxidative damage.

The biflavonoids procyanidin, fukugetin, amentoflavone and podocarpusflavone isolated from the ethyl acetate extract of the leaves of Garcinia brasiliensis have been found to exhibit antioxidant capacity at 10 µM (equivalent to 0.01 mM) with an average inhibition by 28, 42, 37 and 30%, respectively, those values are smaller than the activity observed in the quercetin group (100 µM; 47%) [5]. In this study, we found an average inhibition for the agathisflavone at 0.928 mM by 88%. It seems, the biflavonoid agathisflavone may be a potent antioxidant.

Conclusion

The antioxidant capacity investigated of the agathisflavone applying in vitro test systems allows to conclude that the this biflavonoid delay or prevent significantly the lipid peroxidation and the other referred molecules induced by free radicals, since, the compound showed an antioxidant capacity in DPPH·, ABTS·+, OH·, NO and reduction potential tests. The oxidative damage is linked to inducing damages in the brain and other organs, leading to varieties of health effects in human and other animals. The agathisflavone may be a new hope in the context of drug discovery and development, especially with the importance of the prevention and treatment of diseases related to oxidative stress.

References

  1. 1.

    Barreiros ALBS, David JM, David JP (2006) Oxidative stress: relations between the formation of reactive species and the organism’s defense. Quim Nova 29(1):113–123

  2. 2.

    Adegoke O, Forbes PBC (2015) Challenges and advances in quantum dot fluorescent probes to detect reactive oxygen and nitrogen species: a review. Anal Chim Acta 862:1–13

  3. 3.

    Machado KC, Oliveira GLS, de Sousa ÉBV, Costa IHF, Machado KC, de Sousa DP, Satyald P, Freitaset RM (2015) Spectroscopic studies on the in vitro antioxidant capacity of isopentyl ferulate. Chem Biol Interact 225:47–53

  4. 4.

    Al-Gubory KH, Fowler PA, Garrel C (2010) The roles of cellular reactive oxygen species, oxidative stress and antioxidants in pregnancy outcomes. Int J Biochem Cell Biol 42(10):1634–1650

  5. 5.

    Arwa PS, Zeraik ML, Ximenes VF, da Fonseca LM, da Bolzani VS, Silva DHS (2015) Redox-active biflavonoids from Garcinia brasiliensis as inhibitors of neutrophil oxidative burst and human erythrocyte membrane damage. J Ethnopharmacol 174:410–418

  6. 6.

    Surveswaran S, Cai YZ, Corke H, Sun M (2007) Systematic evaluation of natural phenolic antioxidants from 133 Indian medicinal plants. Food Chem 102(3):938–953

  7. 7.

    Jabbari M, Jabbari A (2016) Antioxidant potential and DPPH radical scavenging kinetics of water-insoluble flavonoid naringenin in aqueous solution of micelles. Colloid Surf A Physicochem Eng Aspects 489:392–399

  8. 8.

    Shi Q, Chen J, Zhou Q, Lei H, Luan L, Liu X, Wu Y (2015) Indirect identification of antioxidants in Polygalae Radix through their reaction with 2,2-diphenyl-1-picrylhydrazyl and subsequent HPLC–ESI-Q-TOF-MS/MS. Talanta 144:830–835

  9. 9.

    Gontijo VS, Judice WAS, Codonho B, Pereira IO, Assis DM, Januário JP, Caroselli EE, Juliano MA, de Carvalho Dosatti A, Marques MJ, Viegas Junior C, Henrique dos Santos M (2012) Leishmanicidal, antiproteolytic and antioxidant evaluation of natural biflavonoids isolated from Garcinia brasiliensis and their semisynthetic derivatives. Eur J Med Chem 58:613–623

  10. 10.

    Barreca D, Gattuso G, Laganà G, Leuzzi U, Bellocco E (2016) C- and O-glycosyl flavonoids in Sanguinello and Tarocco blood orange (Citrus sinensis (L.) Osbeck) juice: identification and influence on antioxidant properties and acetylcholinesterase activity. Food Chem 196:619–627

  11. 11.

    de Freitas RL, Oliveira GL, de Freitas RM, Barreiros AL, David JP, Alves CQ, Pinto CR, David JM (2014) In vitro effects of arylhydrocoumarin on free radicals and oxidative stress in erythrocytes and Saccharomyces cerevisiae. Curr Pharm Biotechnol 15(11):1069–1082

  12. 12.

    Yeh PH, Shieh YD, Hsu LC, Kuo LMY, Lin JH, Liaw CC et al (2012) Naturally occurring cytotoxic [3′→8″]-biflavonoids from Podocarpus nakaii. J Tradit Complement Med 2(3):220–226

  13. 13.

    Kang SS, Lee JY, Choi YK, Song SS, Kim JS, Jeon SJ, Han YN, Sonc KH, Han BH (2005) Neuroprotective effects of naturally occurring biflavonoids. Bioorg Med Chem Lett 15(15):3588–3591

  14. 14.

    Castardo JC, Prudente AS, Ferreira J, Guimarães CL, Monache FD, Filho VC, Otuki MF, Cabrini DA (2008) Anti-inflammatory effects of hydroalcoholic extract and two biflavonoids from Garcinia gardneriana leaves in mouse paw oedema. J Ethnopharmacol 118(3):405–411

  15. 15.

    Jia BX, Zeng XL, Ren FX, Jia L, Chen XQ, Yang J, Liu HM, Wang Q (2014) Baeckeins F–I, four novel C-methylated biflavonoids from the roots of Baeckea frutescens and their anti-inflammatory activities. Food Chem 155:31–37

  16. 16.

    von Moltke LL, Weemhoff JL, Bedir E, Khan IA, Harmatz JS, Goldman P, Greenblatt DJ (2004) Inhibition of human cytochromes P450 by components of Ginkgo biloba. J Pharm Pharmacol 56(8):1039–1044

  17. 17.

    Lin YM, Flavin MT, Schure R, Zembower DE, Zhao GX (1999) Biflavanoids and derivatives there of as antiviral agents. US n. US5948918, 21 June 1996, 07 Sept 1999

  18. 18.

    Freitas AM, Almeida MT, Andrighetti-Fröhner CR, Cardozo FT, Barardi CR, Farias MR, Simões CM (2009) Antiviral activity-guided fractionation from Araucaria angustifolia leaves extract. J Ethnopharmacol 126(3):512–517

  19. 19.

    Gomes NGM, Campos MG, Órfão JMC, Ribeiro CAF (2009) Plants with neurobiological activity as potential targets for drug discovery. Prog Neuro-Psychopharmacol Biol Psychiatry 33(8):1372–1389

  20. 20.

    Mbaveng AT, Hamm R (2014) Harmful and protective effects of terpenoids from African medicinal plants. In: Kuete V (ed) Toxicological survey of African medicinal plants, 1st edn. Toxicological survey of african medicinal plants. Elsevier, New York, pp 557–576

  21. 21.

    PubChem (2015). Pub Chem—open chemistry. Compound summary for CID 5281599—agathisflavone. www.pubchem.ncbi.nlm.nih.gov. Accessed 24 Aug 2015

  22. 22.

    Shrestha S, Lee DY, Park JH, Cho JG, Lee DS, Li B, Kim YC, Jeon YJ, Yeon SW, Baek NI (2013) Flavonoids from the fruits of Nepalese sumac (Rhus parviflora) attenuate glutamate-induced neurotoxicity in HT22 cells. Food Sci Biotechnol 22(4):895–902

  23. 23.

    Shrestha S, Natarajan S, Park JH, Lee DY, Cho JG, Kim GS, Jeon YJ, Yeon SW, Yang DC, Baek NI (2013) Potential neuroprotective flavonoid-based inhibitors of CDK5/p25 from Rhus parviflora. Bioorg Med Chem Lett 23(18):5150–5154

  24. 24.

    Fidelis QC, Ribeiro TAN, Araújo MF, de Carvalho MG (2014) Ouratea genus: chemical and pharmacological aspects. Braz J Pharmacogn 24(1):1–19

  25. 25.

    Ajileye OO, Obuotor EM, Akinkunmi EO, Aderogba MA (2015) Isolation and characterization of antioxidant and antimicrobial compounds from Anacardium occidentale L. (Anacardiaceae) leaf extract. J King Saud Univ Sci 27(3):244–252

  26. 26.

    de Sousa LR, Wu H, Nebo L, Fernandes JB, da Silva MF, Kiefer W, Kanitz M, Bodem J, Diederich WE, Schirmeister T, Vieira PC (2015) Flavonoids as noncompetitive inhibitors of dengue virus NS2B-NS3 protease: inhibition kinetics and docking studies. Bioorg Med Chem 23(3):466–470

  27. 27.

    Pegnyemb DE, Mbing JN, Atchadé ADT, Tih RG, Sondengam BL, Blond A, Bodo B (2005) Antimicrobial biflavonoids from the aerial parts of Ouratea sulcata. Phytochemistry 66(16):1922–1926

  28. 28.

    Paulsen BS, Souza CS, Chicaybam L, Bonamino MH, Bahia M, Costa SL, Borges HL, Rehen SK (2011) Agathisflavone enhances retinoic acid-induced neurogenesis and its receptors α and β in pluripotent stem cells. Stem Cells Dev 20(10):1711–1721

  29. 29.

    Machado KC, Machado KC, Gomes Júnior AL, de Freitas RM (2014) Pharmaceutical application of protease inhibitors: an forecasting technology. Geintec 4(2):780–787

  30. 30.

    Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26(9–10):1231–1237

  31. 31.

    Lopes GKB, Schulman HM, Hermes-Lima M (1999) Polyphenol tannic acid inhibits hydroxyl radical formation from Fenton reaction by complexing ferrous ions. Biochim Biophys ACTA 1479(1–2):142–152

  32. 32.

    Singhal M, Paul A, Singh HP (2014) Synthesis and reducing power assay of methyl semicarbazone derivatives. J Saudi Chem Soc 18(2):121–127

  33. 33.

    Basu S, Hazra B (2006) Review of pharmacological effects of Glycyrrhiza radix and its bioactive compounds. Phyther Res 34(21):2695–2700

  34. 34.

    Guimarães AG, Oliveira GF, Melo MS, Cavalcanti SCH, Antoniolli AR, Bonjardim LR, Silva FA, Santos JPA, Rocha RF, Moreira JCF, Araffljo AAS, Gelain DP, Quintans-Júnior LJ (2010) Bioassay-guided evaluation of antioxidant and antinociceptive activities of carvacrol. Basic Clin Pharmacol Toxicol 107(6):949–957

  35. 35.

    Saxena A, Saxena AK, Singh J, Bhushan S (2010) Natural antioxidants synergistically enhance the anticancer potential of AP9-cd, a novel lignan composition from Cedrus deodara in human leukemia HL-60 cells. Chem Biol Interact 188(3):580–590

  36. 36.

    Zhang D, Tan LH, Wang G, Yua B, Zhao SP, Wang JW, Yao L, Cao WG (2016) Comparative analyses of flavonoids compositions and antioxidant activities of Hawk tea from six botanical origins. Ind Crops Prod 80:123–130

  37. 37.

    Gulcin I, Zubeyr H, Elmastas M, Aboul-Enein HY (2010) Radical scavenging and antioxidant activity of tannic acid. Arab J Chem 3(1):43–53

  38. 38.

    Musa KH, Abdullah A, Kuswandi B, Hidayat MA (2013) A novel high throughput method based on the DPPH dry reagent array for determination of antioxidant activity. Food Chem 141(4):4102–4106

  39. 39.

    Ye Y, Guo Y, Luo Y, Wang Y (2012) Isolation and free radical scavenging activities of a novel biflavonoid from the shells of Camellia oleifera Abel. Fitoterapia 83(8):1585–1589

  40. 40.

    Abad LV, Relleve LS, Racadio CDT, Aranilla CT, Rosa M (2013) Antioxidant activity potential of gamma irradiated carrageenan. Appl Radiat Isot 79:73–79

  41. 41.

    Wojtunik-kulesza KA, Oniszczuk A, Oniszczuk T, Waksmundzka-hajnos M (2016) The influence of common free radicals and antioxidants on development of Alzheimer’s disease. Biomed Pharmacother 78:39–49

  42. 42.

    Tsai M, Huang T (2015) Thiobarbituric acid reactive substances (TBARS) is a state biomarker of oxidative stress in bipolar patients in a manic phase. J Affect Disord 173:22–26

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Authors’ contributions

AWLA—Lab test, data collection & Writing of the draft; KCM—Data analysis & Writing of the draft; KCM—Lab test & Data collection; DDRF—Lab test & Data manipulation; JMD—Data manipulation & Data analysis; MTI—Data presentation, Writing & Revision; SJU—Writing & Revision; JAS—Writing & Revision; JPC—Work design, Writing final draft & Supervision. All authors read and approved the final manuscript.

Acknowledgements

The authors are grateful to the researchers of the Laboratory of Research in Experimental Neurochemistry (LAPNEX/UFPI), Fundação de Amparo à Pesquisa do Estado do Piauí (FAPEPI—Brazil) for materials, equipments, funds and collaborations.

Competing interests

The authors declare that they have no competing interests.

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Affiliations

  1. Laboratory of Research in Experimental Neurochemistry, Federal University of Piauí (UFPI), Teresina, Brazil

    • Anderson Wilbur Lopes Andrade
    • , Keylla da Conceição Machado
    • , Katia da Conceição Machado
    •  & Jéssica Pereira Costa

  2. Chemistry Institute, Federal University of Bahia (UFBA), Salvador, Brazil

    • Daiana Dias Ribeiro Figueiredo
    •  & Jorge Mauricio David

  3. Department for Management of Science and Technology Development, Ton Duc Thang University, Ho Chi Minh City, Vietnam

    • Muhammad Torequl Islam

  4. Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City, Vietnam

    • Muhammad Torequl Islam

  5. Pharmacy Discipline, School of Life Sciences, Khulna University, Khulna, 9208, Bangladesh

    • Shaikh Jamal Uddin
    •  & Jamil A. Shilpi

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Correspondence to Muhammad Torequl Islam.

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Keywords

  • Caesalpinia pyramidalis
  • Radical scavenging
  • Reducing power
  • Lipid peroxidation

BMC Chemistry

ISSN: 2661-801X

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