- Research article
- Open Access
Investigation of supramolecular synthons and structural characterisation of aminopyridine-carboxylic acid derivatives
© Hemamalini et al.; licensee Chemistry Central Ltd. 2014
- Received: 5 December 2013
- Accepted: 22 April 2014
- Published: 6 May 2014
Co-crystal is a structurally homogeneous crystalline material that contains two or more neutral building blocks that are present in definite stoichiometric amounts. The main advantage of co-crystals is their ability to generate a variety of solid forms of a drug that have distinct physicochemical properties from the solid co-crystal components. In the present investigation, five co-crystals containing 2-amino-6-chloropyridine (AMPY) moiety were synthesized and characterized.
The crystal structure of 2-amino-6-chloropyridine (AMPY) (I), and the robustness of pyridine-acid supramolecular synthon were discussed in four stoichiometry co-crystals of AMPY…BA (II), AMPY…2ABA (III), AMPY…3CLBA (IV) and AMPY…4NBA (V). The abbreviated designations used are benzoic acid (BA), 2-aminobenzoic acid (2ABA), 3-chlorobenzoic acid (3CLBA) and 4-nitrobenzoic acid (4NBA). All the crystalline materials have been characterized by 1HNMR, 13CNMR, IR, photoluminescence, TEM analysis and X-ray diffraction. The supramolecular assembly of each co-crystal is analyzed and discussed.
Extensive N---H · · · N/N---H · · · O/O---H · · · N hydrogen bonds are found in (I-V), featuring different supramolecular synthons. In the crystal structure, for compound (I), the 2-amino-6-chloropyridine molecules are linked together into centrosymmetric dimers by hydrogen bonds to form homosynthon, whereas for compounds (II-V), the carboxylic group of the respective acids (benzoic acid, 2-aminobenzoic acid, 3-chlorobenzoic acid and 4-nitrobenzoic acid) interacts with pyridine molecule in a linear fashion through a pair of N---H · · · O and O---H · · · N hydrogen bonds, generating cyclic hydrogen-bonded motifs with the graph-set notation, to form heterosynthon. In compound (II), another intermolecular N---H · · · O hydrogen bonds further link these heterosynthons into zig-zag chains. Whereas in compounds (IV) and (V), these heterosynthons are centrosymmetrically paired via N---H · · · O hydrogen bonds and each forms a complementary DADA [D = donor and A = acceptor] array of quadruple hydrogen bonds, with graph-set notation, and .
- Benzoic Acid
- Transmission Electron Micrographs
- Cambridge Crystallographic Data Centre
- Hydrogen Bond Geometry
- Supramolecular Synthons
Co-crystal is a structurally homogeneous crystalline material that contains two or more neutral building blocks that are present in definite stoichiometric amounts. The main advantage of co-crystals is their ability to generate a variety of solid forms of a drug that have distinct physicochemical properties from the solid co-crystal components. Such properties include, but are not limited to, solubility, dissolution, bioavailability, hygroscopicity, hydrate/solvate formation, crystal morphology, fusion properties, chemical and thermal stabilities, and mechanical properties. Understanding the knowledge of supramolecular synthons is important for hydrogen bond construction. There are two types of synthons which are supramolecular homosynthon (composed of self-complementary functional groups, as exemplified by the carboxylic acid dimer) and supramolecular heterosynthon  (composed of different but complementary functional groups). For instance, the latter includes acid…pyridine , acid…amide [3, 4], hydroxyl…amine  and hydroxyl…pyridine  supramolecular synthons with typical distance ranges for these frequent supramolecular heterosynthons are ca. 2.5-2.8 Å, 2.4-2.8 Å, 2.5-3.0 Å, and 2.5-3.1 Å, respectively. The crucial use of 2-aminopyridine is as an intermediate in the manufacture of pharmaceuticals, particularly in anti-histamines and piroxican. Lornoxican and Tenoxican are considered as new non-steroidal and anti-inflammatory drugs of the oxicam class inhibiting cyclooxygenase which is the key enzyme of prostaglandin biosynthesis at the site of inflammation . The aminopyridine–carboxylate/carboxylic acid systems may adopt two different proton-limiting structures, namely, O---H · · · N (1) → O----H · · · N+(2), which yield hydrogen-bonding and ionic interactions, respectively. These two types of configurations can be represented by the graph-set designator . This motif [robust motif] has been observed in DHFR-TMP [2,4-diamino-5-(3′,4′,5′-trimethoxybenzylpyrimidine)] complexes  and it is one of the 24-most frequently observed cyclic-hydrogen bonded motifs in organic crystal structures . The various hydrogen-bonding patterns involving aminopyrimidine–carboxylate interactions have been reported in the literatures . Many of the recurring hydrogen-bonded motifs leading to supramolecular architectures play a significant role in crystal engineering [12, 13]. The study of co-crystals is of sprouting interest since Active Pharmaceutical Ingredient (API) properties can be modified in a graded manner by revolving into co-crystals . In the present investigation, we have chosen 2-amino-6-chloropyridine (AMPY) (its neutral form) (I), because the molecules of this ligand are self-assembled via N---H · · · N hydrogen bonds to form homosynthon. It also interacts with carboxylic acid molecules through N---H · · · O hydrogen bonds, to form heterosynthon, and paired centrosymmetrically via another N---H · · · O hydrogen bonds, to form a DADA array by multiple hydrogen bonds. The later is a habitually occurring synthon which occurs in amine-carboxylic acid systems. The carboxylic acids referred to in this study, together with their abbreviated designations, are: benzoic acid (BA), 2-aminobenzoic acid (2ABA), 3-chlorobenzoic acid (3CLBA) and 4-nitrobenzoic acid (4NBA). The co-crystals were analyzed by IR spectroscopy, 1HNMR, 13CNMR, photoluminescence, TEM analysis and X-ray diffraction.
Crystallographic data for compounds (I-V)
CCDC deposition number
Dcalc (g cm-3)
Crystal dimensions (mm)
0.33 × 0.28 × 0.15
0.51 × 0.11 × 0.06
0.43 × 0.16 × 0.04
0.58 × 0.15 × 0.03
0.47 × 0.08 × 0.05
Radiation λ (Å)
Ranges/indices (h, k, l)
-16, 16; -6, 6;
-8, 8; -16, 20;
-24, 23; -7, 7;
-5, 5; -18, 18;
-9, 9; -9, 8;
θ limit (°)
(I > 2σ(I))
Goodness of fit on F2
R1, wR2 [I ≥ 2σ(I)]
Hydrogen-bond geometries for compounds (I-V)
D–H · · · A
d(H · · · A) (Å)
d(D · · · A) (Å)
Angle(D–H · · · A) (°)
N2—H1N2 · · · N2i
N2—H2N2 · · · N1ii
C4—H4A · · · Cl1iii
O1—H1N2 · · · O2
N2—H1N2 · · · O2iv
N2—H2N2 · · · O2
N2—H1N2 · · · O2v
O1—H1O1 · · · N1vi
N3—H2N3 · · · O2
O1—H1O1 · · · N1
N2—H1N2 · · · O2vii
N2—H2N2 · · · O2
N2—H2AB · · · O2Aviii
N2A—H2AC · · · O2Aix
O1A—H1OA · · · N1Ax
O1B—H1OB · · · N1Bviii
C3A—H3AA · · · O3Bxi
In all compounds (I-V), atoms in the pyridine ring are coplanar with maximum deviations of 0.005 (1) Å (I), 0.004 (2) Å (II), 0.009 (5) Å (III), 0.008 (2) Å (IV) and 0.002 (3) Å (molecule A):0.007 (2) Å (molecule B) (V), respectively. The C5-N2 [1.3674 (16) Å (I), 1.340 (2) Å (II), 1.360 (8) Å (III), 1.354 (2) Å (IV) and 1.355 (4) Å (molecule A): 1.345 (4) Å (molecule B) (V)] bond lengths are approximately equal to that of a C = N double bond, indicating that atom N2 of the exo amine group must also be sp2 hybridized. This is further supported by the C5—N2—H1N2/H2N2 angles which are in the range of 115.1-120.0° and the fact that atoms C5, N2, H1N2 and H2N2 lie almost in the pyridine plane. Similar bond distances and angles have been observed in 2-aminopyridinium succinate-succinic acid . Proton transfer does not take place from the carboxylic acid to the N atom of 2-amino-6-chloropyridine ring, and the internal C1—N1—C5 angles are 116.8 (1)° (I), 117.1 (2)° (II), 116. 5 (4)° (III), 117.1 (1)° (IV) and 117.1 (3)° (molecule A):117.3 (2)° (molecule B) (V). Compound (III) is a non-merohedral twin with the refined ratio of twin components being 0.276 (6):0.724(6). In (IV), a significant structural change in 3-chlorobenzoic acid has been observed with C-Cl (1.748 (2) Å) and C = O (1.220 (2) Å) bonds which have adopted a cisoid conformation that differ from the pure 3-chlorobenzoic acid which is in a transoid conformation . In (V), the nitro group of the 4-nitrobenzoic acid molecule is twisted slightly from the attached ring and the dihedral angles between N1/C1—C5 and O3—O4/C3/N1 planes are 5.91 (16)° (molecule A) and 5.79 (15)° (molecule B).
In this article, 2-amino-6-chloropyridine and its four co-crystals of benzoic acid derivatives were structurally characterized. It was observed that homosynthon was presented in crystal structure (I), whereas carboxylic acid…pyridine heterosynthon were formed in all four co-crystals structures (II)-(V). DADA arrays were observed in the crystal structures of (IV) and (V). These DADA arrays have been observed in many 2,4-diaminopyrimidine carboxylate complexes as this motif is a potentially recurring synthon. Common laboratory analytical tools such as 1H NMR, 13CNMR, IR, photoluminescence, TEM analysis and XRD were used to understand the supramolecular architectures and to confirm the formation of the co-crystals. All co-crystals display photoluminescence in the solid state. The emission colours of the AMPY-BA derivatives-based building modules are significantly influenced by their incorporation of co-formers into the co-crystals.
Materials and methods
2-Amino-6-chloropyridine (AMPY) (I) was used in this study. AMPY was reacted with a series of benzoic acid and its derivatives to form the following co-crystals: AMPY…BA (II), AMPY…2ABA (III), AMPY…3CLBA (IV) and AMPY…4NBA (V).
Synthesis of (I-V)
Hot methanol solution of 2-amino-6-chloropyridine (AMPY) (I) (57 mg, Aldrich) was warmed over a heating magnetic stirrer for 5 minutes. The resulting solution was allowed to cool slowly at room temperature. Crystals of the compound (I) appeared from the mother liquor after a few days. Compounds (II-V) were prepared by the mixing of hot methanolic solutions of AMPY (Sigma Aldrich, Malaysia) and the corresponding benzoic acid and its derivatives [2-amino benzoic acid, 3-chlorobenzoic acid and 4-nitrobenzoic acid (Sigma Aldrich, Malaysia) in a 1:1 molar ratio. The resultant mixtures were warmed over a water bath at 80˚C for 20 min, allowed to cool slowly and kept at room temperature for crystallization. After a few days, crystals of (II-V) were obtained.
Transmission Fourier Transform Infrared (FTIR) spectroscopy
Transmission FTIR spectra were recorded on a PERKIN ELMER SPECTRUM GX (Perkin- Elmer Instruments LLC, Shelton, CT, USA). The KBr sample disk was scanned with a scan number of 8 from 400 to 4000 cm-1 having a resolution of 4 cm-1.
1H and 13C NMR spectroscopy
1H-NMR and 13C-NMR spectra were recorded at 400 MHz, in DMSO-d6, on Fourier transform Bruker spectrometer. The chemical shifts are reported in part per million (ppm) downfield from internal tetramethylsilane (TMS) (chemical shift in δ values). The spectroscopic details of NMR are summarized in Additional file 1: Table S2 and S3 (†ESI). The 1HNMR and 13CNMR spectra were shown in Additional file 1: Figures S2 and S3 (†ESI).
Optical (OM) & Transmission Electron (TEM) Microscopes
An optical microscope (SZII; Olympus, Tokyo, Japan) equipped with a CCD camera (SSC-DC50A; SONY, Tokyo, Japan) was used to take images of crystal habit. Transmission Electron Micrographs (TEM) were obtained using a Philips TEM CM12 with an image analysis system. The specimen was prepared by depositing a drop of the alcholic solution of I-V suspension on the graphite grid sample holder and gently dried.
PL spectra at room temperature of the samples were measured by Jobin Yvon HR 800 UV using 325 nm line of a He–Cd laser and Ar laser as the excitation source respectively. An analyzer was used to select the transverse-electric mode of the scattering light. Polarization-dependent PL spectra were performed at 15 K with a frequency-doubled Nd+-YAG laser at 532 nm as excitation source. The collected PL light was dispersed through a 0.5 m monochromator equipped with a 300 gr/mm grating and detected by an extended-InGaAs detector (detecting range: 0.5–1.1 eV). A linear polarizer was utilized to analyze the polarization of luminescence, and a depolarizer was placed in between the polarizer and monochromator to eliminate the response from the grating. In order to confirm the repeatability, the measurements were carried out for three times. Since the difference between the results was minimum (<0.1%), only one data from each measurement is presented for discussion.
Powder X-ray Diffraction (XRPD)
XRPD diffractogram at 25˚C provided another piece of information for the identification and crystallinity of starting materials and co-crystals. Moreover, the powder diffraction patterns generated with the single-crystal data of compounds (I-V) using Mercury  matches accurately these experimental XRPD spectra measured using the D5000 powder diffractometer, thereby confirming the purity of the synthesized co-crystals. XRPD diffractograms were collected by SIEMENS D5000 DIFFRACTOMETER. The source of XRPD was CuKα (1.542 Å) and the diffractometer was operated at 40 kV and 30 mA. The X-ray was passed through a 1 mm slit and the signal a 1 mm slit, a nickel filter, and another 0.1 mm slit. The detector type was a scintillation counter. The scanning rate was set at 0.05° ranging from 5° to 35°. The quantity of sample used was around 20–30 mg.
Single-Crystal X-ray data collection and structure determinations
Compounds (I-V) were examined under a microscope, and suitable single crystals were selected for X-ray analysis. Data were collected on a Bruker APEX2 CCD diffractometer with monochromatized MoKα radiation (λ = 0.71073 Å) equipped with an Oxford Cryo-system Cobra low-temperature attachment. Data for (I-V) were collected at 100 K. Lattice parameters were determined from least-squares analysis, and reflection data were integrated using the program SAINT. Lorentz and polarization corrections were applied for diffracted reflections. In addition, the data were corrected for absorption using SADABS. Structures were solved by direct methods and refined by full-matrix least-squares based on F2 using SHELXTL. Molecular graphics: SHELXTL, software used to prepare material for publication: SHELXTL and PLATON . N- and O- bound hydrogen atoms were located from the difference Fourier map, and were refined with a riding model with Uiso(H) = 1.2 or 1.5 Ueq(N, O). The remaining hydrogen atoms in all the compounds (I-V) were positioned geometrically and refined as riding on their parent atoms, with U iso (H) = 1.2 Ueq(C). Crystallographic data for compounds (I-V) are presented in Table 1, whereas hydrogen bond geometries are listed in Table 2.
These data (CCDC 806013 (I), CCDC 806014 (II), CCDC 806010 (III), CCDC 806011 (IV) and CCDC 806012 (V)) can be obtained free of charge at http://www.ccdc.cam.ac.uk/conts/retrieving.html/ or from the Cambridge Crystallographic Data Centre (CCDC), 12 Union Road, Cambridge CB2 IEZ, UK; fax: +44(0) 1223–336033; e-mail: firstname.lastname@example.org.
†ESI: Electronic Supporting Information.
HKF and CKQ thank Universiti Sains Malaysia (USM) for the APEX DE2012 grant (No.1002/PFIZIK/910323) and RUC grant (Structure Determination of 50 kDa Outer Membrane Proteins from S. typhi by X-ray Protein Crystallography, No. 1001/PSKBP/8630013). MH thanks Universiti Sains Malaysia for a post-doctoral research fellowship (2009-2012). WSL thanks Malaysian Government for MyBrain15 (MyPhD) scholarship. The authors extend their appreciation to The Deanship of Scientific Research at King Saud University for the funding the work through the research group project No. RGP-VPP-207.
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