- Research article
- Open Access
Study on the stability and antioxidant effect of the Allium ursinumwatery extract
© Putnoky et al.; licensee Chemistry Central Ltd. 2013
- Received: 20 December 2012
- Accepted: 29 January 2013
- Published: 1 February 2013
Organosulfur compounds usually present a reduced stability especially in the presence of oxygen. This research aimed to study the stability and antioxidant potential of the Allium ursinum watery extract.
The decrease of the antioxidant capacity verifies an exponential relation which may be formally associated to a kinetically pseudomonomolecular process. The exponential regression equation allows the half-life of the degradation process to be determined, this being 14 hours and 49 minutes in a watery environment at room temperature.
The watery extract of Allium ursinum changes its proprieties in time. This might be explained by the network of hydrogen bonds in a watery environment which has a protective effect on the dissolved allicin molecule.
- Antioxidant Capacity
- Dimethyl Disulfide
- Organosulfur Compound
The species belonging to the Allium family have been used for a long time as a remedy for the prevention and treatment of certain diseases . The adjectives associated to these plants i.e. spicy, imposing, distinct and even the latin name Allium, deriving from the Celtic “all” meaning pungent, reflects the presence of certain flavours and scents, all sharing a single element: sulfur. Many of these sulfur compounds contain the allyl group, name deriving from Allium. The very presence of these organosulfur compounds defines the character of this species. The wide spectrum of therapeutic actions has been attributed to organosulfur substances [2, 3]. The chemistry of Alliaceae offers examples of organosulfur compounds with an amazing physiological activity, organosulfur intermediate compounds with unusual bonds [4, 5], challenging analytical problems, stereochemical characteristics related to the presence of sulfur, unusual organosulfur heterocycles with important spectroscopic properties, redox reactions involving sulfurs, pericyclic reactions in organo-synthetical chemistry [6, 7]. The first exhaustive synthesis on these compounds was published in 1992 . This paper, besides presenting an impressive number of chemically characterised compounds, confirms previous data according to which the main component with a therapeutic action is allicin [9, 10]. A. ursinum (ramson) and other representatives of the Allium species, contain 1–5% nonprotein secondary metabolites of aminosulfuric acids . In the cell, the stable precursor of the antibacterial principle of Cavallito, the S–oxyde (+/−) of S–2–propenyl cysteine and the S–oxyde of S–alkenyl cysteine (odour and flavour precursors) are located in the cytoplasm while the enzyme alliinase in the vacuole . Both in the paper by Block  as well as in previous published works, two beneficial properties of this component are highlighted: the antibacterial effect and the antioxidant capacity (the property to bind reactive free radicals) [14, 15]. Using chromatographic assays, the active components were isolated and subsequently identified. Analyses by high-performance liquid chromatography suggested that these compounds were sulfur components, with a characteristic absorbance at 250 nm. Gas chromatography–mass spectrometry analyses allowed the chemical structures of the isolated components to be investigated [3, 16]. Ramson, garlic and onion extracts have been used in popular medicine, and commercial products of these plants record an increasing use.
According to epidemiologic evidence, low cancer risks are associated to a high intake of alliaceae [17, 18], reason for which health organizations recommend the use of A. ursinum as raw material in diets aiming to prevent cellular malignancy. For all these reasons, a detailed research of the organosulfur chemistry of the genus Allium seems justified [19, 20]. Literature data states that in a watery solution, allicin presents an increased stability as compared to other solvents (e.g. alcohol, acetone etc.) .
The purpose of the present study is the watery extract of A. ursinum. This research intends to study the stability of the allicin containing watery extract of A. ursinum.
The degradation in time of this compound affects the stability and the period of use of ramson preparations, and as such, a significant study of this degradation process is not without interest.
The spectrophotometric study of the extract
Determination and characterization of the antioxidant capacity
Values of induction times (IT) and effective induction times (EIT)
nmol vitamin C
Decrease in time of the antioxidant activity of the A. ursinum watery extract
Time of degradation (ore)
Nmol vitamin C
Using the literature value of the specific absorbance at 240 nm (145.4 dL/g · cm), the allicin content reported to the used raw material could be determined i.e. 0.694 μg/ml.
For both it performs a calibration curve and estimate the advance between the integral under the curve of the blank (solution without antioxidant) and sample (standard solution or extract with antioxidant-plant) and distribute by the standard integral.
These estimates are done automatically by the software unit.
The intensity of the light signal, measured by a photomultiplier, depends on the speed of the processes [F] and [G]. If the system does not include a compound capable to bond free radicals, the entire amount of generated anion superoxide is consumed by the light supplying agent “luminol”, and the intensity of the emitted light is maximal.
If the system contains an amount of free radical binding agents (antioxinants) a competition between luminol and the free radical binding agents occurs for the superoxide anion radical. In this case the light signal detected by the photomultiplier has a lower intensity . The unimolecular decomposition of methyl methane thiosulfate is favoured by the weak S–S bond which has the energy of 46 +/−4.6 kcal/mol comparable to the energy for the dissociation of the S–S bond in dimethyl trisulfure but considerably lower than in dimethyl disulfide (75 kcal/mol) .
By comparison, the S–S bonding energies in phenyl benzene tiosulfinate and diphenyl disulfur are 35 and 65 kcal/mol, respectively. The oxygen in tiosulfinate participates in the hydrogen bond, even if not as strong as the oxygen in sulfoxydes . By using the strength of the hydrogen bond, metan tiosulfinate, ethylethane thiosulfinate and dimetyl sulfoxide were demonstrated to cause 278, 308 and 360 cm–1 changes, respectively, in the vibration frequency of the bond. In order to answer the question on the way to benefit from an Alliaceae diet, the following recommendations may be formulated: The texture of Alliaceae species is completely crushed when these are consumed, thus allowing a large amount of S–oxyde S–alkenylcysteine to enter unchanged into the intestinal tract. As alliinase are ireversibly inactivated by the gastric pH, sulfoxides are cleaved by intestinal bacteria into corresponding disulfures . Thiosulfinates which reach the stomach survive the low pH long enough to attack existing pathogenic microorganisms. The allk(en)yltiolation of certain SH biological groups by thiosulfinates, di– or polysulfures or ajoene is beneficial (tiamine allyl tiolation occurs rapidly with the generation of allyl tiamine which is easier absorbed from the intestine than tiamine). The disulfures and other sulfur compounds formed in Alliaceae species play a role in the intestinal tract by deactivation of nitrites or other environmental toxins . The metabolic pathway of Alliaceae organosulfur compounds represents an important field for future research.
The study confirms the literature statement that the A. ursinum watery extract has an antioxidant capacity lasting for several hours. The half-time, as experimentally determined in this study, is 15 hours and 45 minutes at room temperature.
As such, after 24 hours, the antioxidant capacity of an A. ursinum watery extract is decreased. Observing the degradation process in time, an exponential relation was described which may formally correspond to a kinetically pseudomonomolecular model. Even if absorbance reported to specific wavelengths correspond to the domain indicated in literature for the allicin component, the lack of a well-defined chromophore in the allicin molecule does not allow an effective monitoring of the degradation process. Unlike the UV study, monitoring the antioxidant capacity by chemiluminescence method represents a rapid, comfortable and adequate technique for monitoring allicin changes in a watery environment.
Equipments and substances
UV absorption spectra were recorded with a PG Instruments UV–VIS spectrophotometer using the UV WIN 5.05 software. The antioxidant capacity was determined by chemiluminiscence using the “PHOTOCHEM” dedicated device produced by Jena Analytic, Germany. The standard substances were purchased from Sigma Aldrich.
Obtaining the extract
A fresh ramson bulb was collected from April to May in deciduous forests (western part of Romania) weighed with the semi micro analytical scale (7.00 g) is crushed in a grinding mortar and homogenized in 50 mL distilled water. The homogenized matter is quantitatively transformed in a 250 mL measuring bottle and the volume is completed with distilled water. The non-homogeneous content of the bottle is filtered during several stages, using filtration membranes with increasingly lower porosity (osmonics–type membranes, manufactured by Micron Separations Inc.).
The spectrophotometric study of the extract
The filtrate is diluted 30 times with distilled water to obtain the working solution. The UV absorption spectrum of the working solution was obtained against distilled water in a 1 cm quartz cuvette. In order to argument the identity of the absorbing species (allicin), the absorbance report measured at 240–254 nm is determined according to literature specifications. For the fresh watery extract, this report is 1.442, value situated in the 1.4–1.5 interval also indicated by literature data .
Determination and characterization of the antioxidative capacity
Eight 2 mL aliquots from the working solution are frozen at −10°C. By successively defreezing these aliquots, the antioxidant capacity is measured every 3 hours. The determination of antioxidant capacity is based upon the photochemical generation of the superoxide anion radical initiated by a 185–240 nm UV beam.
The authors thank Prof. Zoltan Szabadai, MD, PhD. for helpful discussions and critical reading of the manuscript.
- Bagheri F, Gol A, Dabiri S, Javadi A: Preventive effect of garlic juice on renal reperfusion injury. Iran J Kidney Dis. 2011, 5: 194-200.Google Scholar
- Ray B, Chauhan NB, Lahiri DK: Oxidative insults to neurons and synapse are prevented by aged garlic extract and S–allyl–L–cysteine treatment in the neuronal culture and APP–Tg mouse model. J Neurochem. 2011, 117: 388-402. 10.1111/j.1471-4159.2010.07145.x.View ArticleGoogle Scholar
- Bagiu RV, Vlaicu B, Butnariu M: Chemical composition and in vitro antifungal activity screening of the allium ursinum L. (Liliaceae). Int J Mol Sci. 2012, 13: 1426-1436. 10.3390/ijms13021426.View ArticleGoogle Scholar
- Stajner D, Popović BM, Canadanović-Brunet J, Stajner M: Antioxidant and scavenger activities of Allium ursinum. Fitoterapia. 2008, 79: 303-305. 10.1016/j.fitote.2007.01.008.View ArticleGoogle Scholar
- Sapunjieva T, Alexieva I, Mihaylova D, Popova A: Antimicrobial and antioxidant activity of extracts of Allium ursinum L. 2012, J BioSci Biotech, 143-145.Google Scholar
- Touloupakis E, Ghanotakis DF: Nutraceutical use of garlic sulfur–containing compounds. Adv Exp Med Biol. 2010, 698: 110-121. 10.1007/978-1-4419-7347-4_9.View ArticleGoogle Scholar
- Iciek M, Kwiecień I, Włodek L: Biological properties of garlic and garlic–derived organosulfur compounds. Environ Mol Mutagen. 2009, 50: 247-265. 10.1002/em.20474.View ArticleGoogle Scholar
- Wabwoba BW, Anjili CO, Ngeiywa MM, Ngure PK, Kigondu EM, Ingonga J, Makwali J: Experimental chemotherapy with Allium sativum (Liliaceae) methanolic extract in rodents infected with Leishmania major and Leishmania donovani. J Vector Borne Dis. 2010, 47: 160-167.Google Scholar
- Kubec R, Krejcová P, Simek P, Václavík L, Hajslová J, Schraml J: Precursors and formation of pyrithione and other pyridyl-containing sulfur compounds in drumstick onion. Allium stipitatum. J Agric Food Chem. 2011, 59: 5763-5770. 10.1021/jf200704n.View ArticleGoogle Scholar
- Block E: The organosulfur chemistry of the genus allium–implications for the organic chemistry of sulfur. Angew. Chem Int Ed Engl. 1992, 31: 1135-1178. 10.1002/anie.199211351.View ArticleGoogle Scholar
- Robène-Soustrade I, Legrand D, Gagnevin L, Chiroleu F, Laurent A, Pruvost O: Multiplex nested PCR for detection of Xanthomonas axonopodis pv. allii from onion seeds. Appl Environ Microbiol. 2010, 76: 2697-2703. 10.1128/AEM.02697-09.View ArticleGoogle Scholar
- Musah RA, He Q, Kubec R: Discovery and characterization of a novel lachrymatory factor synthase in Petiveria alliacea and its influence on alliinase–mediated formation of biologically active organosulfur compounds. Plant Physiol. 2009, 151: 1294-1303. 10.1104/pp.109.142539.View ArticleGoogle Scholar
- Sengupta A, Ghosh S, Bhattacharjee S: Allium vegetables in cancer prevention: an overview. Asian Pac J Cancer Prev. 2004, 5: 237-245.Google Scholar
- Young-Jik K: Effects of dietary supplementation of garlic by-products on total phenol contents, DPPH radical scavenging activity, and physicochemical properties of chicken meat. Korean J Food Sci Anim Resour. 2010, 30: 860-866. 10.5851/kosfa.2010.30.5.860.View ArticleGoogle Scholar
- Jung-Hye S, Duck-Joo C, Soo-Jung L, Ji-Young C, Jeong-Gyun K, Nak-Ju S: Changes of physicochemical components and antioxidant activity of garlic during its processing. J Life Sci. 2008, 18: 1123-1131. 10.5352/JLS.2008.18.8.1123.View ArticleGoogle Scholar
- O’Donnell G, Poeschl R, Zimhony O, Gunaratnam M, Moreira JB, Neidle S, Evangelopoulos D, Bhakta S, Malkinson JP, Boshoff HI, Lenaerts A, Gibbons S: Bioactive pyridine–N–oxide disulfides from Allium stipitatum. J Nat Prod. 2009, 72: 360-365. 10.1021/np800572r.View ArticleGoogle Scholar
- Kang MH, Park HM: Hypertension after ingestion of baked garlic (Allium sativum) in a dog. J Vet Med Sci. 2010, 72: 515-518. 10.1292/jvms.09-0434.View ArticleGoogle Scholar
- Nasim SA, Dhir B, Samar F, Rashmi K, Mahmooduzzafa Mujib A: Sulphur treatment alters the therapeutic potency of alliin obtained from garlic leaf extract. Food Chem Toxicol. 2009, 47: 888-892. 10.1016/j.fct.2009.01.024.View ArticleGoogle Scholar
- Jong-Kwan J, Se-Young Y, Jin-Su K, Young-Woo K, Ku Y, Il-Kyung K, Byung-Jo C: Effect of garlic extract supplementation on growth performance, nutrient digestibility, carcass characteristics and meat composition in broilers. Korean J Poultry Sci. 2009, 36: 287-292. 10.5536/KJPS.2009.36.4.287.View ArticleGoogle Scholar
- Picard Y, Roumagnac P, Legrand D, Humeau L, Robène-Soustrade I, Chiroleu F, Gagnevin L, Pruvost O: Polyphasic characterization of Xanthomonas axonopodis allii associated with outbreaks of bacterial blight on three Allium species in the Mascarene archipelago. Phytopathology. 2008, 98: 919-925. 10.1094/PHYTO-98-8-0919.View ArticleGoogle Scholar
- Ryzhikov MA, Ryzhikova VO: Application of chemiluminescent methods for analysis of the antioxidant activity of herbal extracts. Vopr Pitan. 2006, 75: 22-26.Google Scholar
- Modelli A, Jones D: Temporary anion states and dissociative electron attachment in diphenyl disulfide. J Phys Chem A. 2006, 110: 10219-10224. 10.1021/jp0628683.View ArticleGoogle Scholar
- Beato MV, Sánchez HA, de Castro A, Montaño A: Effect of processing and storage time on the contents of organosulfur compounds in pickled blanched garlic. J Agric Food Chem. 2012, 60: 3485-3491. 10.1021/jf3002075.View ArticleGoogle Scholar
- Yamaji M, Tojo S, Takehira K, Tobita S, Fujitsuka M, Majima T: S–S bond mesolysis in alpha, alpha’-dinaphthyl disulfide radical anion generated during gamma–radiolysis and pulse radiolysis in organic solution. J Phys Chem A. 2006, 110: 13487-13491. 10.1021/jp0645770.View ArticleGoogle Scholar
- Singh V, Belloir C, Siess MH, Le Bon AM: Inhibition of carcinogen-induced DNA damage in rat liver and colon by garlic powders with varying alliin content. Nutr Cancer. 2006, 55: 178-184. 10.1207/s15327914nc5502_9.View ArticleGoogle Scholar
- Antonello S, Daasbjerg K, Jensen H, Taddei F, Maran F: Formation and cleavage of aromatic disulfide radical anions. J Am Chem Soc. 2003, 125: 14905-14916. 10.1021/ja036380g.View ArticleGoogle Scholar
- Rybak ME, Calvey EM, Harnly JM: Quantitative determination of allicin in garlic: supercritical fluid extraction and standard addition of alliin. J Agric Food Chem. 2004, 52: 682-687. 10.1021/jf034853x.View ArticleGoogle Scholar
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