Skip to main content
  • Research article
  • Open access
  • Published:

Design, synthesis, and biological evaluation of symmetrical azine derivatives as novel tyrosinase inhibitors


A series of symmetrical azine derivatives containing different substituted benzyl moieties were designed, synthesized, and evaluated for their inhibitory activity against tyrosinase. The results showed that compounds 3e, 3f, 3h, 3i, 3j, and 3k possess effective tyrosinase inhibition with IC50 values ranging from 7.30 μM to 62.60 μM. Particularly, compounds 3f displayed around three-fold improvement in the potency (IC50 = 7.30 ± 1.15 μM) compared to that of kojic acid (IC50 = 20.24 ± 2.28 μM) as the positive control. Kinetic study of compound 3f confirmed uncompetitive inhibitory activity towards tyrosinase indicating that it can bind to enzyme–substrate complex. Next, molecular docking analysis was performed to study the interactions and binding mode of the most potent compound 3f in the tyrosinase active site. Besides, the cytotoxicity of 3f, as well as its potency to reduce the melanin content were also measured on invasive melanoma B16F10 cell line. Also, 3f exhibited above 82% cell viability in the A375 cell line at 10 µM. Consequently, compounds 3f could be introduced as a potent tyrosinase inhibitor that might be a promising candidate in the cosmetics, medicine, and food industry.

Peer Review reports


Melanin is known as a major pigment found in the eyes, hair, and skin of animals and humans which has protective roles against the harmful effects of ultraviolet (UV) irradiation, oxidative stress, DNA damage, and malignant transformation [1, 2]. Despite the key features of melanin, excessive production, and hyperpigmentation of melanin cause dermatological disorders such as melasma, ephelides, chloasma, freckles, melanoderma, and senile lentigines [3]. The excess melanin synthesis can also induce inflammation such as eczema, irritant and allergic eczema contact dermatitis, which may be attributed to critical and emotionally distressing difficulty [4]. Moreover, there is some evidence about the correlation between neuromelanin and the pathogenesis of Parkinson’s disease [5]. Also in the agricultural industry, overproduction of melanin in fruits and vegetables causes food browning and decline in product quality [6].

Tyrosinase (Polyphenol oxidase, a copper-containing enzyme, PPO, E.C. is a critical rate-limiting enzyme in the melanogenesis pathway. Tyrosinase plays a central role in the biosynthesis pathway of melanin pigment by catalyzing the hydroxylation and oxidation of monophenols to o-diphenols (monophenolase activity) and the oxidation of o-diphenols to o-quinones (diphenolase activity) [7]. To get rid of the mentioned undesirable problems, searching for novel and effective tyrosinase inhibitors is highly demanded. Although a large number of tyrosinase inhibitors have been discovered [8] such as chalcones [9], stilbenes [10,11,12,13], flavonol [14], flavone [15, 16], flavanone [17], flavanol [18], kojic acid [19,20,21,22,23], tropolone [8, 24,25,26,27], phthalimide [28], triazole [29] thiosemicarbazide [30] and quinazoline [31] derivatives (Fig. 1), only a few of them have been applied to the market due to their harmful and undesirable side effects, such as lack of safety, low efficacy and allergenic reactions [32, 33]. Consequently, it is necessary to search for new and potent tyrosinase inhibitors with fewer adverse effects.

Fig. 1
figure 1

Chemical structures of some tyrosinase inhibitors from natural or synthetic sources

Azines, N–N linked diamines are useful and stable compounds with interesting chemical attributes. Recently, azine-containing compounds gain lots of attention as important scaffold for drug designing owing to their modulating behavior towards bio-receptors [34, 35]. Azines also demonstrated antibacterial, antimalarial, antiviral, antitumor, and anti-inflammatory properties [36, 37].

As a result, in this study, novel series of azine derivatives were rationally designed, synthesized, and evaluated against the tyrosinase enzyme. Molecular docking analysis of the most potent derivative was also obtained to achieve a distinct insight into the binding mode and interactions of the compounds in the tyrosinase enzyme active site. Moreover, the kinetic, cytotoxic, as well as melanin content assays, were also performed.

Results and discussion

Design approach

Resveratrol (3,5,4′-trihydroxy-trans-stilbene) (A) is a natural antioxidant with numerous beneficial effects on human health such as neuroprotective, cardioprotective, anti‐inflammatory, and anti-cancer effects which prompted the use of resveratrol as a therapeutic agent [38, 39]. Resveratrol is also known as a powerful tyrosinase inhibitor with an IC50 value of 26.63 μM with no marked toxicity (main concern at doses of ≥ 0.5 g/day for long-term use) [40].

Oozeki et al. reported resveratrol base structure as strong tyrosinase inhibitors with an IC50 value of 0.37 μM for the most potent compound (compound B, Fig. 2). Their results showed that the symmetry bibenzyl skeleton could easily bind to the active site of the tyrosinase and improved the inhibitory potency [41]. Furthermore, incorporation of an azo group into the linker while keeping bi-aryl structure gave azo-resveratrol derivatives. Particularly, compound C showed IC50 = 36.28 ± 0.72 μM in a dose-dependent manner, comparable to that of resveratrol [42].

Fig. 2
figure 2

Molecular hybridization and fragment-based approach for designing of bis-aryl hydrazine derivatives as tyrosinase inhibitors

Recently, it has been reported that some hydrazine-containing compounds, such as compounds D [43], E [44], and F [45], were effective tyrosinase inhibitors with an IC50 value of 9.9, 1.58, and 0.05 μM, respectively [46, 47]. Considering these structural features of all depicted compounds, most of them contain the same pharmacophore so that a linker with optimum length (two to four atoms) is connected to two aromatic rings (Ring X-Linker-Ring Y).

As a result, molecular hybridization and fragment-based drug design strategy were performed to develop a series of 1,2-bisaryl hydrazine derivatives as tyrosinase inhibitors. Various substituents were performed on benzylidene moieties to define beneficial structure–activity relationships (SARs).


A series of bis aryl hydrazine hybrids (3ak) was synthesized according to the general synthetic route depicted in Scheme 1. Commercially available aryl aldehydes (1) were reacted with hydrazine hydrate (2) in refluxing ethanol for 24 h to give the corresponding compounds (3ak). After cooling, the precipitate was filtered, washed with cold water, and recrystallized in methanol. The structures were fully characterized and confirmed by IR, 1H-NMR, 13C-NMR, MS, and elemental analysis.

Scheme 1
scheme 1

The synthetic route for the synthesis of bis aryl hydrazine derivatives (3ak)

Tyrosinase inhibitory activity

The inhibitory effects of all synthesized derivatives (3ak) on tyrosinase are presented in terms of IC50 in Table 1. Kojic acid was used as a positive control for comparative purposes. In general, six compounds 3e, 3f, 3h, 3i, 3j, and 3k showed considerable inhibitory effects on tyrosinase with IC50 ranging from 7.3 to 62.6 μM.

Table 1 Tyrosinase inhibitory effects of synthesized compounds 3aj in comparison with kojic acid and Binding energy
figure a

The unsubstituted benzyl derivative (3a) had no significant inhibitory activity at the tested concentrations (IC50 > 100 μM).

To investigate the effect of the substituted moiety, different groups were introduced on the benzyl pendant. In the case of mono-substitution, it was found that the presence of one methoxy at different positions of benzyl ring did not show any improvement in the inhibitory activity (3b, R = ortho-OCH3 with IC50 > 100 μM and 3c, R = para-OCH3 with IC50 > 100 μM) compared to unsubstituted one. The isosteric replacement of methoxy at para position (3c), with a hydroxyl group in compound 3j, increased the inhibitory effect against tyrosinase (IC50 = 62.6 ± 0.71 µM).

Besides, compared to mono substitutions, multi-substitutions on benzyl ring significantly enhanced inhibitory potency as can be seen in compounds 3e, 3f, 3h, 3i, and 3k. The exception in this series came back to the compounds 3d (R = 3-OEt-4-OH, IC50 > 100 μM) and 3g (R = 3,4-(OMe)2, IC50 > 100 μM) which depicted weak anti-tyrosinase activity. Particularly, the most potent ligand identified in this study was compound 3f bearing 2,4-dihydroxy moiety with IC50 = 7.30 ± 1.15 µM, followed by 3k (R = 3-OMe-4-OH) with an IC50 value of 12.90 ± 0.18 μM. On the other hand, the substitution of a para hydroxyl group in compound 3k (R = 3-OMe-4-OH, IC50 = 12.9 ± 0.18 μM) with an acetoxy moiety resulted in 3i which showed a significant decrease in the inhibitory effect (IC50 = 28.1 µM). From the screening data, 3e containing 3,4,5-trimethoxy substituted groups at R known as the third potent compound in this series (IC50 = 20.10 ± 0.01 µM) with approximately the same value of potency compared to that of the standard kojic acid with an IC50 value of 20.24 ± 2.28 μM.

Kinetic studies

Kinetic studies were performed to examine the mechanism and type of inhibition by compound 3f as the most potent derivative against tyrosinase. The results are presented in Fig. 3. Lineweaver–Burk plots (plot of 1/V versus 1/[S]) for the inhibition of tyrosinase were obtained with several concentrations of 3f as the inhibitor and L-DOPA as the substrate. The analysis showed that Vmax and Km values decreased in the presence of increasing concentrations of compound 3f. With these results, it can presume that the type of inhibition for compound 3f is uncompetitive.

Fig. 3
figure 3

Lineweaver–Burk plot of mushroom tyrosinase enzyme inhibition by different concentrations of 3f in the presence of L-DOPA as a substrate. The reciprocal tyrosinase inhibitory activity was plotted against the reciprocal substrate concentration (double reciprocal plot, n = 3). Km is the Michaelis–Menten constant and Vmax is the maximum reaction velocity

Molecular docking study

The molecular binding analysis was then performed to gain an understanding of the interactions and binding mode of 3f in the tyrosinase active site. The results are presented in Table 2. Tyrosinase has H2L2 tetramer structure with two H subunits and two L subunits. The active site placed in the H subunit comprises binuclear copper ions so that the first copper ion demonstrated interactions with three histidine residues named His 61, His 85, and His95. The second copper ion coordinated by His 259, His 263, and His 296. Detailed and comprehensive studies confirmed that interactions with these critical residues can completely inactivate the monophenolase and diphenolase activity.

Table 2 Molecular docking results of compound 3f with mushroom tyrosinase (PDB ID: 2Y9X)

As shown in Fig. 4, compound 3f fitted well in the tyrosinase binding pocket by hydrogen bonding, electrostatic and hydrophobic interactions. The nitrogen atoms and the oxygen atoms of 3f formed five hydrogen bonds interacting with the Val 283, Gly 281, His 296, Met 280, and His 259 residues at distances of 2.80 Å, 3.74 Å, 2.78 Å, 2.80 Å, and 3.07 Å, respectively. Moreover, compound 3f was involved in electrostatic interactions with His263 and Arg268 as well as hydrophobic interactions with Phe264, Val283, His263, and Ala286. Docking results indicated that the substitution on the phenyl ring played an important role in forming drug-receptor interactions and binding orientation of the compound in the active site of tyrosinase by enhancing phenyl ring electron density.

Fig. 4
figure 4

The binding orientation (a) and interactions (b) of compound 3f into the tyrosinase enzyme. Ligand 3f is displayed as cyan sticks, while the core residues are shown as green sticks. Hydrogen bonding, electrostatic, Pi–Pi, and alkyl-Pi interactions are displayed as green, orange, pink and light pink, respectively

Cell viability on B16F10

MTT assay was performed to determine the toxicity of 3f as the most potent tyrosinase inhibitor against invasive melanoma B16F10 cell line. According to the toxicity assay, 3f showed 60.15 ± 9.82% (± SD) cell viability at 10 µM.

Measuring cell viability of A375 in response to 3f

The A375 cell line is derived from a human skin melanoma carrying two mutant genes, B-RAF and CDKN2 which are associated with melanoma of sun-damaged skin [48]. To determine the cytotoxicity of 3f in A375 melanoma cell lines, MTT studies were conducted. Results exhibited that the cell viability was significantly higher in A375 with 82.59 ± 5.85 (± SD) % viability compared with the B16F10 cell line at 10 µM.

Melanin content assay

It is well documented that effective tyrosinase inhibitors may reduce the phenolase activities of the enzyme which in return downregulates the melanogenesis process [49, 50]. In this regard, the potency of 3f to reduce the melanin content was evaluated on the B16F10 cell line. Kojic acid was used as the positive control. Data were expressed as mean ± SD of at least three independent experiments. According to Fig. 5, 3f significantly reduced the melanin content in skin melanoma cells to 79.58% at 10 µM in comparison with the control (P-value < 0.05).

Fig. 5
figure 5

Effect of compound 3f on melanin content in B16F10 melanoma cells


Following our expertise in the rational design of tyrosinase inhibitors; herein, we designed, synthesized, and evaluated different azine substituted derivatives against tyrosinase. The most active compound 3f bearing 2,4-dihydroxy on the benzyl ring (IC50 = 7.30 ± 1.15 μM) showed 3 times better potency compared to that of kojic acid as the positive control. Moreover, it is worth mentioning that 3f showed an uncompetitive inhibition mode of action in the enzymatic assay. Molecular docking analysis demonstrated that the high potential of 3f would be due to the formation of strong interactions with the critical residues of the tyrosinase active site. In addition, cell assessments of 3f at 10 µM against B16F10 and A375 cell lines exhibited around 60% and 82% viability, respectively. Importantly, compound 3f showed approximately similar potency to reduce the melanin content on B16F10 cell lines at 10 µM compared to kojic acid as a positive control. In general, it can be concluded that the synthesized compounds can serve as structural outlines and promising lead to design and expand potential tyrosinase inhibitors.

Material and methods


All needed chemicals were purchased from Merck and Acros chemical companies. All reagents and solvents were dried before use according to standard methods. Mushroom tyrosinase (EC1.14.18.1), kojic acid, dimethyl sulfoxide (DMSO), and l-3,4-dihydroxyphenylalanine (L-DOPA) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). IR spectra were obtained on an FT-IR Perkin-Elmer Precisely system spectrophotometer (potassium bromide disks). Melting points were determined on a Kofler hot stage apparatus and are uncorrected. 1H NMR and 13C NMR spectra were recorded in DMSO using a Bruker Avance DPX instrument (1H NMR 500 MHz, 13C NMR 125 MHz). Chemical shifts (δ) were reported in parts per million (ppm) downfield from TMS as an internal standard. All of the coupling constants (J) are in hertz. The mass spectra were run on an Agilent 6410 apparatus. Merck silica gel 60 F254 plates were used for analytical thin-layer chromatography (TLC). Preparative thin-layer chromatography was done with prepared glass-backed plates (20 * 20 cm2) using silica gel (Merck-Kieselgel 60 HF254, Art. 7739).


The typical procedure for the synthesis of bis aryl hydrazide derivatives

A mixture of aryl aldehyde (1 mmol), and hydrazine hydrate (0.5 mmol) were refluxed in ethanol for 24 h. The reaction proceeding was monitored by TLC (n-hexane/ethyl acetate, 20:1 v/v). After completion of the reaction, the mixture was cooled to room temperature, the precipitated solid was isolated by filtration, all products were recrystallized from hot ethanol. Physical and spectral data of bis aryl hydrazide derivatives are given below [51,52,53].

1,2-di(Benzylidene)hydrazine (3a)

Yellow solid; Isolated yield: 65%; m.p: 88–93 °C; I.R (KBr, cm−1): 3067, 3050, 3025, 3000, 2949, 1624, 1574, 1491, 1446. 1H NMR (300 MHz, DMSO-d6) δH (ppm): 8.58 (s, 2H), 7.98–7.81 (m, 4H), 7.55–7.32 (m, 6H). 13C NMR (75 MHz, DMSO-d6) δc (ppm): 156.30, 134.20, 130.89, 128.76, 128.31. Anal. Calcd. For C14H12N2: C: 80.74; H: 5.81; N: 13.45; Found. C: 80.99; H: 5.83; N: 13.48.

1,2-bis(2-Methoxybenzylidene)hydrazine (3b)

Commercially available with CAS number of 17745-81-2. Yellow solid; isolated yield: 64%; m.p: 142–146 °C; I.R (KBr) Ʋmax (cm−1): 3075, 3005, 2965, 2946, 2842, 1616, 1576, 1485, 1468, 1438, 1321, 1250. 1H NMR (300 MHz, DMSO-d6) δH (ppm): 8.94 (s, 2H), 7.98 (d, J = 7.3 Hz, 2H), 7.51 (t, J = 7.5 Hz, 2H), 7.15 (d, J = 7.5 Hz, 2H), 7.05 (t, J = 7.5 Hz, 2H), 3.89 (s, 6H). 13C NMR (75 MHz, DMSO-d6) δc (ppm): 56.27, 112.49, 121.19, 122.08, 127.00, 133.50, 157.01, 159.22. MS (m/z, %): 77.3(41), 91.3(90), 119.3(65), 131.3(26), 150.3(35), 161.3(13), 237.3(92), 268.3(100). Anal. Calcd. For C16H16N2O2: C: 71.62; H: 6.01; N: 10.44; Found. C: 72.92; H: 6.00; N: 10.45.

1,2-bis(4-Methoxybenzylidene)hydrazine (3c)

Commercially available with CAS number of 2299-73-2. Yellow solid; isolated yield: 58%; m.p: 164–166 °C; I.R (KBr) Ʋmax (cm−1): 3092, 3059, 2982, 2971, 2931, 2878, 1631, 1600, 1474, 1439, 1400, 1377, 1357. 1H NMR (300 MHz, DMSO-d6) δH (ppm): 3.83 (s, 6H), 7.06 (s, 4H), 7.82 (s, 4H), 8.64 (s, 2H). 13C NMR (75 MHz, DMSO-d6) δc (ppm): 55.86, 114.87, 127.03, 130.44, 160.96, 162.15. MS (m/z, %): 77.3(34), 91.3(18), 107.3(42), 121.3(24), 134.3(46), 148.3(26), 161.3(70), 240.4(16), 268.3(M+, 83). Anal. Calcd. For C16H16N2O2: C: 71.62; H: 6.01; N: 10.44; Found. C: 72.92; H: 6.00; N: 10.45.

4,4′-(Hydrazine-1,2-diylidenebis(methaneylylidene))bis(2-ethoxyphenol) (3d)

White solid; isolated yield: 69%; m.p: 210–218 °C; I.R (KBr) Ʋmax (cm−1): 3435, 3292, 3052, 2987, 2968, 2927, 2838, 1620, 1500, 1461, 1420, 1377, 1251. 1H NMR (300 MHz, DMSO-d6) δH (ppm): 1.37 (t, J = 5.4 H), 4.07 (q, J = 5.4 H), 6.90 (d, J = 7.5 Hz, 2H), 7.26 (d, J = 7.5 Hz, 2H), 7.44 (s, 2H), 8.57 (s, 2H), 9.63 (s, 2H). 13C NMR (7.5 MHz, DMSO-d6) δc (ppm): 15.16, 64.30, 111.92, 116.06, 123.76, 126.00, 147.57, 150.55, 161.06. MS (m/z, %): 163.3(19), 191.4(41), 272.4(39), 299.4(23), 328.3(M+, 100). Anal. Calcd. For C18H20N2O4: C: 65.84; H: 6.14; N: 8.53; Found. C: 66.62; H: 6.15; N: 8.52.

1,2-bis(3,4,5-Trimethoxybenzylidene)hydrazine (3e) [51]

Yellow solid; isolated yield: 75%; m.p: 201–205 °C; I.R (KBr) Ʋmax (cm−1): 3083, 3063, 3012, 2989, 2955, 2936, 2840, 1623, 1505, 1457, 1434, 1457, 1375, 1150. 1H NMR (300 MHz, DMSO-d6) δH (ppm): 3.74 (s, 6H), 3.85 (s, 12H), 7.22 (s, 4H), 8.66 (s, 2H). 13C NMR (75 MHz, DMSO-d6) δc (ppm): 56.39, 60.63, 106.03, 129.73, 140.65, 153.63, 161.72. MS (m/z, %): 221.4(25), 345.4(23), 388.4(M+, 100). Anal. Calcd. For C20H24N2O6: C: 61.85; H: 6.23; N: 7.21; Found. C: 69.76; H: 6.24; N: 7.20.

4,4′-(Hydrazine-1,2-diylidenebis(methaneylylidene))bis(benzene-1,3-diol) (3f)

Yellow solid; isolated yield: 92%; m.p: 313 °C; I.R (KBr) Ʋmax (cm−1): 3518, 3468, 3214, 3097, 3096, 2998, 2936, 2891, 1616, 1589, 1534, 1453, 1251, 1121. 1H NMR (300 MHz, DMSO-d6) δH (ppm): 6.35 (d, J = 2.4 Hz, 2H), 6.41 (dd, J = 8.4, 2.4 Hz, 2H), 7.41 (d, J = 8.4 Hz, 2H), 8.77 (s, 2H), 10.27 (s, 2H), 11.39 (s, 2H). 13C NMR (75 MHz, DMSO-d6) δc (ppm): 102.95, 108.69, 110.73, 133.46, 161.16, 162.27, 162.54. MS (m/z, %): 137.3(79), 255.4(36), 272.2(M+, 100). Anal. Calcd. For C14H12N2O4: C: 61.76; H: 4.44; N: 10.29; Found. C: 60.88; H: 4.45; N: 10.28.

1,2-bis(3,4-Dimethoxybenzylidene)hydrazine (3g) [52]

Commercially available with CAS number of 17745-86-7. Yellow solid; isolated yield: 94%; m.p: 197–199 °C; I.R (KBr) Ʋmax (cm−1): 3079, 3003, 2962, 2930, 2840, 1624, 1580, 1542, 1465, 1443, 1345, 1259. 1H NMR (300 MHz, DMSO-d6) δH (ppm): 3.83 (s, 12H), 7.05 (s, 2H), 7.36 (s, 2H), 7.49 (s, 2H), 8.63 (s, 2H). 13C NMR (75 MHz, DMSO-d6) δc (ppm): 55.86, 56.09, 109.52, 111.95, 123.97, 127.16, 134.76, 149.46, 161.25. MS (m/z, %): 79.2(23), 164.3(13), 191.3(52), 286.4(27), 313.4(9), 328.3(M+, 100). Anal. Calcd. For C18H20N2O4: C: 65.84; H: 6.14; N: 8.53; Found. C: 64.96; H: 6.15; N: 8.52.

5,5′-(Hydrazine-1,2-diylidenebis(methaneylylidene))bis(2-methoxyphenol)(3h) [53]

Yellow solid; isolated yield: 73%; m.p: 270–275 °C; I.R (KBr) Ʋmax (cm−1): 3295, 3074, 3009, 2941, 2842, 1619, 1579, 1508, 1461, 1439, 1360, 1273, 1027. 1H NMR (300 MHz, DMSO-d6) δH (ppm): 3.83 (s, 6H), 7.01 (d, J = 8.1 Hz, 2H), 7.23 (d, J = 8.1 Hz, 2H), 7.38 (s, 2H), 8.53 (s, 2H), 9.36 (s, 2H). 13C NMR (75 MHz, DMSO-d6) δc (ppm): 56.05, 112.25, 113.78, 122.29, 127.33, 147.23, 151.08, 161.13. MS (m/z, %): 150.3(12), 177.3(64), 273.3(32), 300.3(M+, 100). Anal. Calcd. For C16H16N2O4: C: 63.99; H: 5.37; N: 9.33; Found. C: 63.79; H: 5.39; N: 9.38.

(Hydrazine-1,2-diylidenebis(methaneylylidene))bis(2-methoxy-4,1-phenylene) diacetate (3i)

Yellow solid; isolated yield: 53%; m.p: 165–170 °C; I.R (KBr) Ʋmax (cm−1): 3075, 3010, 2944, 2887, 2849, 1765, 1632, 1599, 1508, 1474, 1457, 1375, 1272, 1155, 1030, 1010. 1H NMR (300 MHz, DMSO-d6) δH (ppm): 2.29 (s, 6H), 3.86 (s, 6H), 7.25 (d, J = 7.2 Hz, 2H), 7.47 (d, J = 7.2 Hz, 2H), 7.65 (s, 2H), 8.73 (s, 2H). 13C NMR (75 MHz, DMSO-d6) δc (ppm): 20.86, 56.33, 111.54, 122.45, 123.89, 133.11, 142.32, 151.70, 161.36, 168.81. MS (m/z, %): 43.3(57), 177.3(34), 258.4(12), 273.4(23), 300.4(100), 341.5(51), 384.3(M+, 79). Anal. Calcd. For C20H20N2O6: C: 62.49; H: 5.24; N: 7.29; Found. C: 62.47; H: 5.25; N: 7.32.

4,4′-(Hydrazine-1,2-diylidenebis(methaneylylidene))diphenol (3j)

Yellow solid; isolated yield: 78%; m.p: 204–208 °C; I.R (KBr) Ʋmax (cm−1): 3367, 3069, 3012, 2988, 2955, 2839, 1620, 1577, 1490, 1470, 1280, 1089. 1H NMR (300 MHz, DMSO-d6) δH (ppm): 6.87 (d, J = 8.4 Hz, 4H), 7.70 (d, J = 8.4 Hz, 4H), 8.56 (s, 2H), 10.11 (s, 2H). 13C NMR (75 MHz, DMSO-d6) δc (ppm): 116.22, 125.57, 130.56, 160.75, 160.84. MS (m/z, %): 65.3(68), 93.3(22), 120.3(40), 147.3(84), 212.3(27), 223.3(9), 240.3(M+, 100). Anal. Calcd. For C14H12N2O2: C: 69.99; H: 5.03; N: 11.66; Found. C: 69.76; H: 5.09; N: 11.69.

4,4′-(Hydrazine-1,2-diylidenebis(methaneylylidene))bis(2-methoxyphenol) (3k)

Commercially available with CAS number of 1696-60-2, Yellow solid; isolated yield: 62%; m.p: 169–174 °C; I.R (KBr) Ʋmax (cm−1): 3481, 3084, 3003, 2958, 2937, 2921, 2856, 1625, 1602, 1509, 1453, 1427, 1239, 1032. 1H NMR (300 MHz, DMSO-d6) δH (ppm): 3.84 (s, 6H), 6.89 (d, J = 8.4 Hz, 2H), 7.27 (dd, J = 8.4, 1.8 Hz, 2H), 7.47 (d, J = 1.8 Hz, 2H), 8.58 (s, 2H), 9.72 (s, 2H). 13C NMR (75 MHz, DMSO-d6) δc (ppm): 55.97, 110.48, 115.95, 123.96, 125.99, 148.44, 150.34, 161.08. MS (m/z, %): 150.3(12), 177.3(54), 258.4(25), 272.5(18), 299.6(54), 300.3(M+, 100). Anal. Calcd. For C16H16N2O4: C: 63.99; H: 5.37; N: 9.33; Found. C: 63.79; H: 5.39; N: 9.38.

Tyrosinase assay

Mushroom tyrosinase (EC (Sigma Chemical Co.) was assayed as explained previously with slight modifications applying L-DOPA as substrate [54]. In spectrophotometric experiments, enzyme activity was monitored by observing dopachrome formation at 475 nm. The stock solutions of test compounds 3ak and kojic acid were first dissolved in DMSO at 40 mM and then diluted with phosphate buffer (pH = 6.8) to the required concentrations. First, 10 µL of mushroom tyrosinase (0.5 mg mL−1) was mixed with 160 µL of phosphate buffer (50 mM, pH = 6.8) and then 10 µL of the test sample in 96-well microplates was added. After the mixture was pre-incubated at 28 °C for 20 min, 20 µL of L-DOPA solution (0.5 mM) was added to the phosphate buffer and dopachrome formation was monitored at 475 nm for 10 min. DMSO without test compounds was used as the control, and kojic acid was used as the positive control. Each assay was conducted as three separate replicates. The final concentration of DMSO in the test solution was less than 2.0%. The percent inhibition ratio was calculated according to the following equation:

$$ {\text{Inhibition}}\left( \% \right) = 100 \times {{\left( {{\text{Abs}}_{{{\text{control}}}} - {\text{Abs}}_{{{\text{compound}}}} } \right)} \mathord{\left/ {\vphantom {{\left( {{\text{Abs}}_{{{\text{control}}}} - {\text{Abs}}_{{{\text{compound}}}} } \right)} {{\text{Abs}}_{{{\text{control}}}} }}} \right. \kern-\nulldelimiterspace} {{\text{Abs}}_{{{\text{control}}}} }}. $$

The inhibitory activity of the tested compounds was expressed as the concentration that inhibited 50% of the enzyme activity (IC50).

Determination of the inhibition type

A series of experiments were performed to determine the inhibition kinetics of 3f. The inhibitor concentrations were: 0, 8, 16 μM. Substrate (L-DOPA) concentrations were (0.1, 0.25, 0.5, 0.75 and 1.5 mM) in kinetic studies. Pre-incubation and measurement time was the same as discussed in the mushroom tyrosinase inhibition assay protocol. Maximum initial velocity was determined from the initial linear portion of absorbance up to 10 min after addition of L-DOPA with 1 min interval. The Michaelis constant (Km) and the maximal velocity (Vmax) of the tyrosinase activity were determined by the Lineweaver–Burk plot at various concentrations of L-DOPA as a substrate. The inhibition type of the enzyme was assayed by Lineweaver–Burk plots of the inverse of velocities (1/V) versus the inverse of substrate concentrations 1/[S] mM−1.

Molecular docking study

Docking was done by AutoDock 4.2 ( and AutoDock Tools 1.5.4 (ADT) ( X-ray crystal structure of agaricus bisporus tyrosinase containing tropolone in the active site (PDB ID: 2Y9X), was regained from protein data bank ( Before the docking method, the water molecules and the inherent ligand were eliminated from the protein. Hydrogens were attached and non-polar hydrogens were merged. Also, Gasteiger charges were calculated for protein 2Y9X. 3D structures of ligands were drawn and minimized under Molecular Mechanics MM+ and then Semi-empirical AM1 methods using HyperChem software ( The pdbqt formats of the ligands were prepared by adding Gasteiger charges and setting the degree of torsions. The active site which contains Cu2+ metal ions were chosen for docking and the grids’ center was placed on the tropolone’s binding site. The box dimensions were set to 40 × 40 × 40 with 0.375 Å grid spacing. To determine the docking parameter file, a rigid macromolecule was elected. The Lamarckian genetic search algorithm was used and the number of GA runs was determined at 100. The other parameters were left at program default values. The validity of the docking procedure was tested using the co-crystallized inhibitor as ligand and the above-mentioned protocol. Finally, conformations having the lowest assumed free energies of binding were studied to be analyzed [55].

MTT assay for cell viability

The cytotoxic activity of compound was evaluated in B16F10 cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cells were seeded in a 96-well plate (5 × 103 cells/well) and incubated at 37 °C with samples at different concentrations for 48 h. Following the treatment, cells were incubated with MTT (0.5 mg mL−1) at 37 °C for 3 h. The MTT-containing medium was then removed, and 100 μL of DMSO was added to each well, mixed thoroughly with a 10 min shake to dissolve formazan crystals. The absorbance of each well was measured at 540 nm.

Determination of melanin content

B16F10 cells were seeded in six-well plates (1.0 × 105 cells/well). After 24 h, the medium was substituted by a fresh one and treated with 3f, and incubated for 48 h. Then, cells were treated with 100 nM \(\alpha \)-MSH. Kojic acid was used as positive control and for comparing the inhibitory strength of the compound. After incubation cells were washed twice with PBS and harvested using 0.25 M trypsin, then dissolved in 300 μL of 1 N NaOH/10% DMSO buffer and boiled for 2 h at 80 °C to solubilize the melanin. The absorbance of the supernatant was measured at 470 nm in a microplate reader. The obtained results were normalized using total protein content.

Availability of data and materials

The datasets used and analyzed during the current study are available from the corresponding author on reasonable request.


  1. Zhu Y-J, Qiu L, Zhou J-J, Guo H-Y, Hu Y-H, Li Z-C, Wang Q, Chen Q-X, Liu B. Inhibitory effects of hinokitiol on tyrosinase activity and melanin biosynthesis and its antimicrobial activities. J Enzyme Inhib Med Chem. 2010;25(6):798–803.

    CAS  PubMed  Google Scholar 

  2. Brenner M, Hearing VJ. The protective role of melanin against UV damage in human skin. Photochem Photobiol. 2008;84(3):539–49.

    CAS  PubMed  PubMed Central  Google Scholar 

  3. Lerner AB, Fitzpatrick TB. Treatment of melanin hyperpigmentation. J Am Med Assoc. 1953;152(7):577–82.

    CAS  PubMed  Google Scholar 

  4. Kim YJ, Kang KS, Yokozawa T. The anti-melanogenic effect of pycnogenol by its anti-oxidative actions. Food Chem Toxicol. 2008;46(7):2466–71.

    CAS  PubMed  Google Scholar 

  5. Bose A, Petsko GA, Eliezer D. Parkinson’s disease and melanoma: co-occurrence and mechanisms. J Parkinsons Dis. 2018;8(3):385–98.

    PubMed  PubMed Central  Google Scholar 

  6. Taranto F, Pasqualone A, Mangini G, Tripodi P, Miazzi M, Pavan S, Montemurro C. Polyphenol oxidases in crops: biochemical, physiological and genetic aspects. Int J Mol Sci. 2017;18(2):377.

    PubMed Central  Google Scholar 

  7. Seo S-Y, Sharma VK, Sharma N. Mushroom tyrosinase: recent prospects. J Agric Food Chem. 2003;51(10):2837–53.

    CAS  PubMed  Google Scholar 

  8. Roulier B, Pérès B, Haudecoeur R. Advances in the design of genuine human tyrosinase inhibitors for targeting melanogenesis and related pigmentations. J Med Chem. 2020;63(22):13428–43.

    CAS  PubMed  Google Scholar 

  9. Nerya O, Musa R, Khatib S, Tamir S, Vaya J. Chalcones as potent tyrosinase inhibitors: the effect of hydroxyl positions and numbers. Phytochemistry. 2004;65(10):1389–95.

    CAS  PubMed  Google Scholar 

  10. Aggarwal BB, Bhardwaj A, Aggarwal RS, Seeram NP, Shishodia S, Takada Y. Role of resveratrol in prevention and therapy of cancer: preclinical and clinical studies. Anticancer Res. 2004;24:2783–840.

    CAS  PubMed  Google Scholar 

  11. Shin NH, Ryu SY, Choi EJ, Kang SH, Chang IM, Min KR, Kim Y. Oxy-resveratrol as the potent inhibitor on dopa oxidase activity of mushroomtyrosinase. Biochem Biophys Res Commun. 1998;243:801–3.

    CAS  PubMed  Google Scholar 

  12. Shimizu K, Kondo R, Sakai K, Lee SH, Sato H. The inhibitory componentsfrom Artocarpus incisus on melanin biosynthesis. Planta Med. 1998;64:408–12.

    CAS  PubMed  Google Scholar 

  13. Likhitwitayawuid K, Sritularak B. A new dimeric stilbene with tyrosinaseinhibitiory activity from Artocarpus gomezianus. J Nat Prod. 2001;64(11):1457–9.

    CAS  PubMed  Google Scholar 

  14. Xie LP, Chen QX, Huang H, Wang HZ, Zhang RQ. Inhibitory effects of some flavonoids on the activity of mushroom tyrosinase. Biochem Biokhimiia. 2003;68(4):487–91.

    CAS  Google Scholar 

  15. Kubo I, Kinst-Hori I. Flavonols from saffron flower: tyrosinase inhibitory activity and inhibition mechanism. J Agric Food Chem. 1999;47(10):4121–5.

    CAS  PubMed  Google Scholar 

  16. Kwak J, Seok J, Suh HJ, Choi YH, Hong S, Kim D, Boo Y. Antimelanogenic effects of luteolin 7-sulfate isolated from Phyllospadix iwatensis Makino. Br J Dermatol. 2016;175(3):501–11.

    CAS  PubMed  Google Scholar 

  17. Jeong SH, Ryu YB, Curtis-Long MJ, Ryu HW, Baek YS, Kang JE, Lee WS, Park KH. Tyrosinase inhibitory polyphenols from roots of Morus lhou. J Agric Food Chem. 2009;57(4):1195–203.

    CAS  PubMed  Google Scholar 

  18. Zheng ZP, Cheng KW, To JT, Li H, Wang M. Isolation of tyrosinase inhibitors from Artocarpus heterophyllus and use of its extract as antibrowning agent. Mol Nutr Food Res. 2008;52(12):1530–8.

    CAS  PubMed  Google Scholar 

  19. Burdock GA, Soni MG, Carabin IG. Evaluation of health aspects of kojic acid in food. Regul Toxicol Pharmacol. 2001;33(1):80–101.

    CAS  PubMed  Google Scholar 

  20. Bentley R. From miso, sake and shoyu to cosmetics: a century of science forkojic acid. Nat Prod Rep. 2006;23:1046–62.

    CAS  PubMed  Google Scholar 

  21. Lajis AFB, Hamid M, Ariff AB. Depigmenting effect of kojic acid esters inhyperpigmented B16F1 melanoma cells. J Biomed Biotechnol. 2012.

    Article  PubMed  PubMed Central  Google Scholar 

  22. Molenda JJ, Basinger MA, Hanusa TP, Jones MM. Synthesis and iron(III) binding properties of 3-hydroxypyrid-4-ones derived from kojic acid. J Inorg Biochem. 1994;55(2):131–46.

    CAS  PubMed  Google Scholar 

  23. Kim H, Choi J, Cho JK, Kim SY, Lee Y-S. Solid-phase synthesis of kojic acid-tripeptides and their tyrosinase inhibitory activity, storage stability, and toxicity. Bioorg Med Chem Lett. 2004;14(11):2843–6.

    CAS  PubMed  Google Scholar 

  24. Ismaya WT, Rozeboom HJ, Weijn A, Mes JJ, Fusetti F, Wichers HJ, Dijkstra BW. Crystal structure of Agaricus bisporus mushroom tyrosinase: identity of the tetramer subunits and interaction with tropolone. Biochemistry. 2011;50:5477–86.

    CAS  PubMed  Google Scholar 

  25. Flurkey A, Cooksey J, Reddy A, Spoonmore K, Rescigno A, Inlow J, Flurkey WH. Enzyme, protein, carbohydrate, and phenolic contaminants in commercial tyrosinase preparations: potential problems affecting tyrosinase activity and inhibition studies. J Agric Food Chem. 2008;56(12):4760–8.

    CAS  PubMed  Google Scholar 

  26. Kahn V, Andrawis A. Inhibition of mushroom tyrosinase by tropolone. Phytochemistry. 1985;24(5):905–8.

    CAS  Google Scholar 

  27. Neeley E, Fritch G, Fuller A, Wolfe J, Wright J, Flurkey W. Variations in IC50 values with purity of mushroom tyrosinase. Int J Mol Sci. 2009;10(9):3811–23.

    CAS  PubMed  PubMed Central  Google Scholar 

  28. Tehrani MB, Emani P, Rezaei Z, Khoshneviszadeh M, Ebrahimi M, Edraki N, Mahdavi M, Larijani B, Ranjbar S, Foroumadi A, et al. Phthalimide-1,2,3-triazole hybrid compounds as tyrosinase inhibitors; synthesis, biological evaluation and molecular docking analysis. J Mol Struct. 2019;1176:86–93.

    CAS  Google Scholar 

  29. Akın Ş, Demir EA, Colak A, Kolcuoglu Y, Yildirim N, Bekircan O. Synthesis, biological activities and molecular docking studies of some novel 2,4,5-trisubstituted-1,2,4-triazole-3-one derivatives as potent tyrosinase inhibitors. J Mol Struct. 2019;1175:280–6.

    Google Scholar 

  30. Hosseinpoor H, Iraji A, Edraki N, Pirhadi S, Attarroshan M, Khoshneviszadeh M, Khoshneviszadeh M. A series of benzylidenes linked to hydrazine-1-carbothioamide as tyrosinase inhibitors: synthesis, biological evaluation and structure−activity relationship. Chem Biodivers. 2020;17(8):e2000285.

    CAS  PubMed  Google Scholar 

  31. Sepehri N, Iraji A, Yavari A, Asgari MS, Zamani S, Hosseini S, Bahadorikhalili S, Pirhadi S, Larijani B, Khoshneviszadeh M, et al. The natural-based optimization of kojic acid conjugated to different thio-quinazolinones as potential anti-melanogenesis agents with tyrosinase inhibitory activity. Bioorg Med Chem. 2021;36:116044.

    CAS  PubMed  Google Scholar 

  32. Chiari ME, Vera DMA, Palacios SM, Carpinella MC. Tyrosinase inhibitory activity of a 6-isoprenoid-substituted flavanone isolated from Dalea elegans. Bioorg Med Chem. 2011;19(11):3474–82.

    CAS  PubMed  Google Scholar 

  33. Karioti A, Protopappa A, Megoulas N, Skaltsa H. Identification of tyrosinase inhibitors from Marrubium velutinum and Marrubium cylleneum. Bioorg Med Chem. 2007;15(7):2708–14.

    CAS  PubMed  Google Scholar 

  34. Kurteva VB, Simeonov SP, Stoilova-Disheva M. Symmetrical acyclic aryl aldazines with antibacterial and antifungal activity. Pharmacol Pharm. 2011;2(01):1.

    CAS  Google Scholar 

  35. Picón-Ferrer I, Hueso-Ureña F, Illán-Cabeza NA, Jiménez-Pulido SB, Martínez-Martos JM, Ramírez-Expósito MJ, Moreno-Carretero MN. Chloro-fac-tricarbonylrhenium (I) complexes of asymmetric azines derived from 6-acetyl-1, 3, 7-trimethylpteridine-2, 4 (1H, 3H)-dione with hydrazine and aromatic aldehydes: preparation, structural characterization and biological activity against several human tumor cell lines. J Inorg Biochem. 2009;103(1):94–100.

    PubMed  Google Scholar 

  36. Jayabharathi J, Thanikachalam V, Thangamani A, Padmavathy M. Synthesis, AM 1 calculation, and biological studies of thiopyran-4-one and their azine derivatives. Med Chem Res. 2007;16(6):266–79.

    CAS  Google Scholar 

  37. Easmon J, Pürstinger G, Heinisch G, Roth T, Fiebig HH, Holzer W, Jäger W, Jenny M, Hofmann J. Synthesis, cytotoxicity, and antitumor activity of copper (II) and iron (II) complexes of 4 N-azabicyclo [3.2.2] nonane thiosemicarbazones derived from acyl diazines. J Med Chem. 2001;44(13):2164–71.

    CAS  PubMed  Google Scholar 

  38. Berman AY, Motechin RA, Wiesenfeld MY, Holz MK. The therapeutic potential of resveratrol: a review of clinical trials. NPJ Precis Oncol. 2017;1(1):1–9.

    Google Scholar 

  39. Singh AP, Singh R, Verma SS, Rai V, Kaschula CH, Maiti P, Gupta SC. Health benefits of resveratrol: evidence from clinical studies. Med Res Rev. 2019;39(5):1851–91.

    CAS  PubMed  Google Scholar 

  40. Cottart C-H, Nivet-Antoine V, Laguillier-Morizot C, Beaudeux J-L. Resveratrol bioavailability and toxicity in humans. Mol Nutr Food Res. 2010;54(1):7–16.

    CAS  PubMed  Google Scholar 

  41. Oozeki H, Tajima R, Nihei K-I. Molecular design of potent tyrosinase inhibitors having the bibenzyl skeleton. Bioorg Med Chem Lett. 2008;18(19):5252–4.

    CAS  PubMed  Google Scholar 

  42. Song YM, Ha YM, Kim J-A, Chung KW, Uehara Y, Lee KJ, Chun P, Byun Y, Chung HY, Moon HR. Synthesis of novel azo-resveratrol, azo-oxyresveratrol and their derivatives as potent tyrosinase inhibitors. Bioorg Med Chem Lett. 2012;22(24):7451–5.

    CAS  PubMed  Google Scholar 

  43. Iraji A, Khoshneviszadeh M, Bakhshizadeh P, Edraki N, Khoshneviszadeh M. Structure-based design, synthesis, biological evaluation and molecular docking study of 4-hydroxy-N′-methylenebenzohydrazide derivatives acting as tyrosinase inhibitors with potentiate anti-melanogenesis activities. Med Chem. 2020;16(7):892–902.

    CAS  PubMed  Google Scholar 

  44. Iraji A, Adelpour T, Edraki N, Khoshneviszadeh M, Miri R, Khoshneviszadeh M. Synthesis, biological evaluation and molecular docking analysis of vaniline–benzylidenehydrazine hybrids as potent tyrosinase inhibitors. BMC Chem. 2020;14:1–11.

    Google Scholar 

  45. Hosseinpoor H, Iraji A, Edraki N, Pirhadi S, Attarroshan M, Khoshneviszadeh M, Khoshneviszadeh M. A series of benzylidene linked to hydrazinecarbothioamide as tyrosinase inhibitors synthesis, biological evaluation and structure-activity relationship. Chem Biodivers. 2020;17(8):e2000285.

    CAS  PubMed  Google Scholar 

  46. Yi W, Cao R-H, Chen Z-Y, Yu L, Ma L, Song H-C. Design, synthesis and biological evaluation of hydroxy-or methoxy-substituted phenylmethylenethiosemicarbazones as tyrosinase inhibitors. Chem Pharm Bull. 2009;57(11):1273–7.

    CAS  Google Scholar 

  47. Li Q, Yang H, Mo J, Chen Y, Wu Y, Kang C, Sun Y, Sun H. Identification by shape-based virtual screening and evaluation of new tyrosinase inhibitors. PeerJ. 2018;6:e4206.

    PubMed  PubMed Central  Google Scholar 

  48. Avram S, Coricovac D-E, Pavel IZ, Pinzaru I, Ghiulai R, Baderca F, Soica C, Muntean D, Branisteanu DE, Spandidos DA, et al. Standardization of A375 human melanoma models on chicken embryo chorioallantoic membrane and Balb/c nude mice. Oncol Rep. 2017;38(1):89–99.

    CAS  PubMed  PubMed Central  Google Scholar 

  49. Chang T-S. Natural melanogenesis inhibitors acting through the down-regulation of tyrosinase activity. Materials. 2012;5(9):1661–85.

    CAS  PubMed Central  Google Scholar 

  50. Pillaiyar T, Manickam M, Jung S-H. Downregulation of melanogenesis: drug discovery and therapeutic options. Drug Discov Today. 2017;22(2):282–98.

    CAS  PubMed  Google Scholar 

  51. Hamid MHSA, Ali MA, Mirza AH, Len GA, Butcher RJ. (1E,2E)-1,2-Bis(2,3,4-trimeth-oxy-benzyl-idene)hydrazine. Acta Crystallogr Sect E Struct Rep Online. 2010;66(Pt 10):o2557–o2557.

    CAS  PubMed  PubMed Central  Google Scholar 

  52. Parveen M, Azaz S, Malla AM, Ahmad F, Pereira da Silva PS, Ramos Silva M. Solvent-free, [Et3NH][HSO4] catalyzed facile synthesis of hydrazone derivatives. New J Chem. 2015;39(1):469–81.

    CAS  Google Scholar 

  53. Shahzad D, Saeed A. Novel C-2 symmetric molecules as α-glucosidase and α-amylase inhibitors: design, synthesis, kinetic evaluation, molecular docking and pharmacokinetics. Molecules. 2019;24(8):1511.

    CAS  PubMed Central  Google Scholar 

  54. Karimian S, Ranjbar S, Dadfar M, Khoshneviszadeh M, Gholampour M, Sakhteman A, Khoshneviszadeh M. 4 H-benzochromene derivatives as novel tyrosinase inhibitors and radical scavengers: synthesis, biological evaluation, and molecular docking analysis. Mol Divers. 2020.

    Article  PubMed  Google Scholar 

  55. Iraji A, Panahi Z, Edraki N, Khoshneviszadeh M, Khoshneviszadeh M. Design, synthesis, in vitro and in silico studies of novel Schiff base derivatives of 2-hydroxy-4-methoxybenzamide as tyrosinase inhibitors. Drug Dev Res. 2021;82(4):533–42.

    CAS  PubMed  Google Scholar 

Download references


Not applicable.


The authors wish to thank the support of the Vice-Chancellor for Research of Shiraz University of Medical Sciences (Grant Number: 97-01-05-18440). This agency was not involved in the design of the study and collection, analysis, and interpretation of data as well as in writing the manuscript. This study was part of the Pharm.D thesis of Fatemeh Kazemi.

Author information

Authors and Affiliations



SK synthesized compounds as well as prepare the manuscript. FK synthesized compounds. MA performed the biological assay. MG performed the biological assay. SH supervised the biological tests. AS contributed to the design and characterization of compounds. YB performed the biological assay. MKabiri performed the biological assay. AI performed docking study and contributed to the preparation of the manuscript. MKhoshneviszadeh supervised all phases of the study. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Aida Iraji or Mehdi Khoshneviszadeh.

Ethics declarations

Ethics approval and consent to participate

Not applicable.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Karimian, S., Kazemi, F., Attarroshan, M. et al. Design, synthesis, and biological evaluation of symmetrical azine derivatives as novel tyrosinase inhibitors. BMC Chemistry 15, 54 (2021).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: