- Research article
- Open Access
Sustained release formulation of an anti-tuberculosis drug based on para-amino salicylic acid-zinc layered hydroxide nanocomposite
© Saifullah et al.; licensee Chemistry Central Ltd. 2013
- Received: 16 December 2012
- Accepted: 25 March 2013
- Published: 20 April 2013
Tuberculosis (TB), is caused by the bacteria, Mycobacterium tuberculosis and its a threat to humans since centuries. Depending on the type of TB, its treatment can last for 6–24 months which is a major cause for patients non-compliance and treatment failure. Many adverse effects are associated with the currently available TB medicines, and there has been no new anti-tuberculosis drug on the market for more than 50 year, as the drug development is very lengthy and budget consuming process.
Development of the biocompatible nano drug delivery systems with the ability to minimize the side effects of the drugs, protection of the drug from enzymatic degradation. And most importantly the drug delivery systems which can deliver the drug at target site would increase the therapeutic efficacy. Nanovehicles with their tendency to release the drug in a sustained manner would result in the bioavalibilty of the drugs in the body for a longer period of time and this would reduce the dosing frequency in drug administration. The biocompatible nanovehicles with the properties like sustained release of drug of the target site, protection of the drug from physio-chemical degradation, reduction in dosing frequency, and prolong bioavailability of drug in the body would result in the shortening of the treatment duration. All of these factors would improve the patient compliance with chemotherapy of TB.
An anti-tuberculosis drug, 4-amino salicylic acid (4-ASA) was successfully intercalated into the interlamellae of zinc layered hydroxide (ZLH) via direct reaction with zinc oxide suspension. The X-ray diffraction patterns and FTIR analyses indicate that the molecule was successfully intercalated into the ZLH interlayer space with an average basal spacing of 24 Å. Furthermore, TGA and DTG results show that the drug 4-ASA is stabilized in the interlayers by electrostatic interaction. The release of 4-ASA from the nanocomposite was found to be in a sustained manner. The nanocomposite treated with normal 3T3 cells shows it reduces cell viability in a dose- and time-dependent manner.
Sustained release formulation of the nanocomposite, 4-ASA intercalated into zinc layered hydroxides, with its ease of preparation, sustained release of the active and less-toxic to the cell is a step forward for a more patient-friendly chemotherapy of Tuberculosis.
- Para-Amino salicylic acid(PAS / 4-ASA)
- Zinc layered hydroxide
- 3T3 cell lines
Tuberculosis (TB) is one of the transmittable diseases caused by the bacteria, Mycobacterium tuberculosis. Despite the technological advancements, tuberculosis is still lethal and the main cause of the human deaths in many countries around the world. According to the latest facts and figure about TB in the Global tuberculosis report 2012, there were about 8.7 millions new cases of TB in 2011 and 1.4 million humans lost their lives. TB is one of the top killers of women, with 300 000 deaths among HIV-negative women and 200 000 deaths among HIV-positive women in 2011 .
There are three forms of TB, namely drug susceptible-, multidrug resistant- and extensively resistant-TB. Drug susceptible TB is mainly treated with first line anti-TB drugs such as Rifampin, Isoniazid, Ethambutol and Pyrazinamide, and treatment usually lasts from 6–9 months.
When tuberculosis bacteria develop resistance to at least two most powerful first line drugs Rifampicin (RMP) and Isoniazid (INH), it is called multi-drug resistant tuberculosis (MDR-TB) . MDR-TB patients' treatment can last for 20 months . 4-amino salicylic acid is a bacteriostatic agent and is extremely helpful in controlling the growth of bacteria. It is also very useful in avoiding the development of the resistance of bacteria to the other anti-MDR-TB drugs . MDR-TB patients have to take 4 g dose of 4-ASA twice daily during treatment . As we know that the drug development is a time consuming process and requires a huge amount of budget and no new anti-TB drug has been introduced in the market for almost 50 years, therefore, the logical approach in such circumstances is that we should use the currently available drugs more intelligently and effectively.
4-aminosalicylic acid (4-ASA) was introduced in 1948 in clinical trials and was found to be very effective against TB . However, due to its side effects such as anorexia, epigastria distress, nausea, vomiting, abdominal cramps or diarrhea, it is no longer used for drug susceptible TB, but is used for the multi-drug resistant TB and Controlled release formulation would be very useful to resuce the side effects associated with 4-ASA [6, 7]. In order to avoid these side effects associated with 4-ASA, various strategies have been adopted by physicians such as the drug given in the form of buffered tablets, granules, coated tablets and salt of acid. The intrinsic half-life of 4-ASA is 45–60 min. The controlled and targeted release can be the best choice to avoid the side effects and would also increase the intrinsic half life of the drug. The sustained release formulation would not only minimize the side effects but would also result in the shortening of the treatment duration and also the dosing frequency. The reduction in the frequency of the dose administration would enhance the patient compliance to the treatment.
Keeping this idea in mind, we have developed the controlled release formulation of 4-amino salicylic acid (4-ASA) with zinc layered hydroxides.
There have been many attempts made earlier by various scientists in the development of drug delivery systems for anti-tuberculosis drugs. Inhalable microparticles of polylactic acid, mannitol microparticles, glucan particles (GPs) and plant proteins etc., have been developed for the delivery of anti-TB drugs, a good review was written by Saifullah et al. Which discusses many of these delivery systems in detail .
Zinc layered hydroxide (ZLH) has the structure similar to that of brucite (Mg (OH) 2 which is a hydrotalcite-like compound and is formed by a layered unit with the metal cations octahedrally coordinated with hydroxyl groups. A quarter of the octahedral of zinc cation coordination is displaced from the main layer to tetrahedral sites located above and below each empty octahedron and can be represented by the general formula of hydrotalcite, , where M2+ is divalent cations, M3+ is trivalent compound and Am- is an exchangeable anion, with the charge, (m-) .
Characteristics of zinc layered hydroxides are similar to that of layered double hydroxides which have plenty of benefits as a drug delivery system, such as their ease of laboratory preparation with controlled particle size . The drugs can easily be accommodated in the space of interlayers by ion exchange as compared to the other nanoparticles which need surface modification i.e. functionalization of the surface [11, 12].
ZLH, have high zeta potential, 20–30 mV like to that of the layered double hydroxides, which is a strong driving force onto the surface of the cell. On the other hand, other inorganic nanoparticles, due to their low solubility, they accumulated in cells. The biocompatibility, controlled release and easier degradation without accumulation in the body are important characteristic of the layered double hydroxides and zinc layered hydroxides, which make them strong candidates as drug delivery systems. In previous studies it has been reported, that the Zn-Al LDH does not show any toxic effect on Chinese hamster ovary cells at low concentration . ZLH has also been used as a drug delivery system of an anticancer compound, ellagic acid . The intercalation of hippuric acid into ZLH has resulted in the less toxic of the compound compared to the pure hippuric acid itself . It was also reported that the controlled release formulation of an antihistamine was obtained by forming the cetirizine-ZLH nanocomposite .
In this study, we report a controlled release formulation of 4-amino salicylic acid, an antituberculosis agent by forming a nanocomposite zinc layered hydroxides(4-ASA-ZLH) nanocomposite.
Analytical grade chemicals were used without any further purification. 4- amino salicylic acid 99% purity and zinc oxide were purchased from Sigma-Aldrich. Dimethyl sulfoxide (DMSO) was used as solvent and was purchased from Ajax Finechem (Sydney, Australia) with 0.1% water content.
The drug, 4-aminosalicyclic acid (0.4 mol) was mixed into 50 mL (i.e 25 mL Deionized water and 25 mL DMSO) and was stirrer for 20 minutes at room temperature. About 0.2 g zinc oxide was mixed with 50 ml deionized water and stirred for 25 minutes. The drug solution was added drop wise into zinc oxide suspension, and the pH of the final solution was raised to 7.9 by drop wise addition of 0.5 M sodium hydroxide solution. The solution was kept on stirring for 18 hours at 70°C. The sample was centrifuged and washed with deionized water and kept in an oven at 70°C for 48 hours and the sample was then subjected to further characterizations [14, 17, 18].
Controlled release study
The release of 4-aminosalicylic acid was performed on different human body simulated pH 4.8 and 7.4 buffer solutions (0.1 M). The phosphate-buffered solution contains a number of anions, such as Cl−, monobasic phosphate H2PO4−, and dibasic phosphate HPO42−. About 0.6 mg of the nanocomposite was placed into 3.5 mL of either a pH 4.8 or pH 7.4 buffer solution. The absorbance were measured at λmax = 268 nm  by a UV–vis absorption spectrophotometer.
To compare the release of 4-aminosalicylic acid from nanocomposite and from a physical mixture of 4-aminosalicylic acid with ZLH, a specific amount of the physical mixture of 4-aminosalicylic and the pristine ZLH was used. The release of the active, 4-aminosalicylic was determined as previously described above.
Healthy 3T3 mouse fibroblast cells were obtained from ATCC (USA). 3T3 fibroblast cells were cultured at 37°C and 5% CO2 in high glucose Dulbecco’s Modified Eagle Medium containing 10% fetal bovine serum, 0.5% penicillin–streptomycin, 1% glutamine, and 1% non-essential amino acids. Cultured cells were passaged using 0.25% trypsin. At 80% confluence, cultured cells were harvested using 0.25% trypsin and were subcultured/seeded in 96-well plates for further characterizations.
Assessment of cytotoxicity by MTT assay
Cytotoxicity of synthesized nanocomposites was evaluated by MTT assay, where the cell viability is spectroscopically measured as the total mitochondrial activity. For MTT assay, cultured 3T3 cells were seeded and cultured in a 96-well culture plate (at a density of 1 × 104 cells per well) in 100 μL cell culture medium (DMEM with 10% fetal bovine serum) and the same were incubated for 24 hours under cultured conditions at 37°C and 5% CO2. Then, cultured cells were washed with phosphate-buffered saline (PBS, pH 7.4) and were then incubated with a medium (100 μL) containing dispersed nanocomposite in various gradient concentrations, range from 0.781 μg/mL to 50 μg/mL. Cells not exposed to nanocomposite were served as control in each experiment. After 24, 48 and 72 hours of incubation, the medium were removed from 96 well plate and incubated with 100 μLof the freshly prepared MTT reagent in DMEM for another 3–4 hours. Observance of the solution in 96 well plates was measured at 570 NM using a microplate reader. Data are expressed as the percentage of cell viability compared with untreated cells under the same experimental conditions.
Shimadzu XRD-6000 diffractometer (Tokyo, Japan) was used to obtain the powder X-ray diffraction (PXRD) patterns. Radiation CuKα at 30 KV and 30 mA was used for recording the PXRD difractograms between 2-60o. Fourier transform infrared (FTIR) spectra of the materials were recorded over the range of 400–4000 cm-1 on a Perkin- Elmer 100 series spectrophotometer by direct sample method. A LECO, model CHNS-932 instrument (St Joseph, MI) was utilized for the analysis of carbon, hydrogen, nitrogen and sulfur (CHNS). A Mettler Toledo instrument (Greifensee, Switzerland), was used for the thermo gravimetric and differential thermo gravimetric analyses (TGA-DTG) analysis. Micromeritics ASAP 2000 (Norcross, GA), was used for the surface area analysis of the samples, by nitrogen gas adsorption–desorption method at 77 K. For the determination of the morphology of the sample surface, a scanning electron microscope (SEM), JOEL JSM-6400 (Tokyo, Japan) was used. A Perkin Elmer UV–vis Spectrophotometer- Lambda was used to determine UV–visible spectra for the optical properties and controlled release studies.
Powder X-ray diffraction
Zinc oxide in aqueous solution formed ZLH layers and at the same time intercalate the anions between the layers, as was proposed earlier . The layers of zinc hydroxide (Zn (OH) 2) were formed on the surface of the solid particles due to hydrolyzed zinc oxide in an aqueous environment. The dissociation of Zn (OH) 2 in a solution-solid interface produces Zn2+ species, which subsequently react with hydroxide group, drug anions and water in solution to form drug-zinc layered hydroxide compound. The Figure 1A shows five intense peaks between 30° and 60°, corresponding to diffractions due to the 100, 002, 101, 102, and 110 planes which characteristic of ZnO.The presence of a peak at a lower 2θ angle with d spacing of 25.2 Å indicates successful intercalation of the 4-amino salicylate anions into the interlayers of zinc-layered hydroxide (Figure 1B). The resulting 4-ASA nanocomposite sample contains a little amount of zinc oxide (indicated by a star). In addition, Figure 1B shows another three harmonics at 2θ = 7.6°, 11.7°, and 14.4°, with d values of 11.6, 7.6, and 6.2, respectively, resulting in an average basal spacing value of 24.0 Å. Some of the 4-amino salicylate anions are also adsorbed on the surface of zinc-layered hydroxide, which are indicated by triangles in the XRD diffractogram of the nanocompsite.
Spatial orientation of intercalated 4-amino salicylic acid
4-amino salicylic acid*
ν as (N-H)
Band for N-H symmetry and antisymmetry are overlapped with O-H stretching at 3345
ν s (N-H)
ν (O-H) in the layer; H 2 O
ν(C = O) in COOH
ν (O-H) ring
δ b (CH), in plane bends
1220, 1109, 813
1200, 1027, 827
ν as (COO - )
ν s (COO - )
The disappearance of a C=O stretching band in the carboxylic group for free 4-amino salicylic acid after intercalation confirms that the species that were intercalated into the ZLH layers are in the anionic form of 4-amino salicylic acid, i.e. 4-amino salicylate. A band at 1549 cm−1 is due to the vas(COO−) mode, and another band at 1372 cm−1 is due to the symmetric vibration of vs (COO−). These two bands indicate that there is an interaction between 4-amino salicylate and ZLH layers. The positions and relative intensities of the other bands almost coincide with those in the spectra of the free 4-amino salicylic acid. A bands due to Zn–OH translation modes are recorded below at 600 cm−1.
Elemental chemical composition of free 4-amino salicylic acid and its nanocomposite
BET surface area m2/g
BJH pore volume cm3/g
BJH Average pore diameter Å
The BJH pore size distributions for zinc oxide and 4-ASA nanocomposite are shown in Figure 6B. All the materials are of a mesoporous-type. The BJH pore size distribution for 4-ASA nanocomposite shows an intense peak at around 110 Å, compared to zinc oxide, which show less intense broad peaks at around 10 and 90 Å. The difference in peak values and pore volume values in the pore size distribution of 4-ASA nanocomposite and zinc oxide indicate the modification of the pure texture which occurred when intercalation of 4-amino salicylic acid took place, in agreement with the formation of the nanocomposite. The BJH average pore diameter and pore volume are shown in Table 2.
Release behavior of 4-amino salicylic acid from 4-ASA nanocomposite
The release profiles of 4-amino salicylic acid from the 4-ASA nanocomposite on exposure to either a pH 4.8 or pH 7.4 environments are shown in Figure 7 (II). The release rate of 4-amino salicylic acid from the nanocomposite is obviously depending on pH; release at pH 7.4 is remarkably slower than that at pH 4.8. The percentage release of 4-amino salicylic acid from the nanocomposite reaches about 100% within about 1330 minutes when exposed to pH 4.8 compared to only about 92% within about 8540 minutes at pH 7.4. The difference in the release rate may be due to possible differences in mechanism [29, 30]. At pH 7.4, ZLH layers are more stable compared to pH 4.8. This indicates that the ZLH layers in acidic media (pH 4.8) dissolve more easily and the release occurs through both the dissolution of ZLH layers and the diffusion. On the other hand at pH 7.4 media, the release occurs through a diffusion mechanism.
The release rate of 4-amino salicylic acid from its nanocomposite was obviously very much slower than that from the physical mixture, indicating that the nanocomposite potentially can be used as a better alternative as compared to the free drug.
Release kinetics of 4-amino salicylic acid from 4-ASA nanocomposite
Usually, the release process of drug molecules from the nanocomposite may be described using pseudo-first order, pseudo-second order or parabolic diffusion kinetic equations.
where q e and q t are the equilibrium release amount and the release amount at any time (t), respectively. The k1 value can be obtained from the slope by plot ln(qe − qt) against t.
where Mo and Mt are the drug content remained in the ZLH at release time 0 and t, respectively.
Correlation coefficient (R 2 ), and rate constants (k) obtained by fitting the data of 4-amino salicylic acid release from 4-ASA nanocomposite into PBS solution at pH 4.8 and 7.4
Saturation release (%)
Parabolic diffusion model
rate constant (k) (mg/min)
3.9 × 10-5
1.0 × 10-4
Cytotoxicity analysis of 4-amino salicylic acid (4-ASA) and 4-ASA nanocomposite
A nanocomposite of 4-amino salicylic-ZLH was obtained by the intercalation of 4-amino salicylic acid anions into zinc layered hydroxide using zinc oxide as starting material. XRD technique was used to confirm the intercalation 4-ASA. The resulting nanocomposite has a basal spacing of 24.0 Å, indicates that the guest adopted a bilayered arrangement. FTIR study shows the presence of functional groups of both 4-amino salicylic acid anions and inorganic host. The thermal stability of intercalated 4-amino salicylic acid anions were enhanced remarkably compared to the pristine 4-amino salicylic acid anions whose decomposition occurs at 227°C. The loading of 4-aminosalicylic acid in the nanocomposite was estimated to be about 16.9% (w/w). The release of 4-amino salicylic acid anions from nanocomposite was found to occur in a controlled manner, so the resulting material is suitable for a controlled-release formulation. Based on preliminary cytotoxicity studies of the nanocomposite, more detailed investigations are needed to elucidate the specific cellular mechanism of toxicity. Further investigations on a broader set of specific cell lines combined with an evaluation of the detailed cellular mechanism of nanocomposite cell interactions are also important. This investigation helps to establish the suitability of pre-clinical model for early toxicity assessment of the nanocomposite. The work on the biocompatible sustained release formulation is a step towards reduction in dosing frequency of the drugs and subsequently enhances the patient compliance and make the tuberculosis chemotherapy patient-friendly.
Funding for this research was provided by the Ministry of Science, Technology and Innovation of Malaysia (MOSTI) under the National Nanotechnology Directorate grant No. NND/NA/1/TD11-010, (UPM Vot No. 5489100) and the Higher Education Commission of Malaysia under the Commonwealth Scholarship and Fellowship Plan (Ref: KPT.B.600-6/3, Vol 68) for Bullo Saifullah.
- Global Tuberculosis P. global tuberculosis control. 2012, WHO report, http://www.who.int/tb/publications/global_report/en/,
- Dalton T, Cegielski P, Akksilp S: Prevalence of and risk factors for resistance to second-line drugs in people with multidrug-resistant tuberculosis in eight countries. a prospective cohort study. Lancet. 2012, 9851: 1406-1417. 10.1016/S0140-6736(12)60734-X. http://press.thelancet.com/TB.pdf,View ArticleGoogle Scholar
- Falzon D, Jaramillo E, Schunemann HJ: WHO guidelines for the programmatic management of drug-resistant tuberculosis. Eur Respir J. 2011, 38 (3): 516-528. 10.1183/09031936.00073611.View ArticleGoogle Scholar
- Jaramillo E: Guidelines for the programmatic management of drug-resistant tuberculosis. 2008, WHO/HTM/TB/2008.402: World Health Organization, http://whqlibdoc.who.int/publications/2008/9789241547581_eng.pdf, 97892 41547581,Google Scholar
- Mitchison DA: Role of individual drugs in the chemotherapy of tuberculosis. Int J Tuberc Lung Dis. 2000, 4 (9): 796-806.Google Scholar
- Boman G: Serum concentration and half-life of rifampicin after simultaneous oral administration of aminosalicylic acid or isoniazid. Eur J Clin Pharmacol. 1974, 7 (3): 217-225. 10.1007/BF00560384.View ArticleGoogle Scholar
- Mitnick C, Bayona J, Palacios E: Community-based therapy for multidrug-resistant tuberculosis in Lima, Peru. N Engl J Med. 2003, 348 (2): 119-128. 10.1056/NEJMoa022928.View ArticleGoogle Scholar
- Bullo S, Hussein MZ, AlAli SH: Controlled release approaches towards the chemotherapy of tuberculosis. Int J Nanomedicine. 2012, 7: 5451-5463.Google Scholar
- Rives V: Layered double hydroxides: present and future. Nova Science Pub Incorporated. 2001, New York: Nova Science Publishers Inc, 439 pp. Price US$ l49-http://books.google.com.br/books?hl=ptBR&lr=&id=U5c5zzqjthgC&oi=fnd&pg=PR7&dq=+Layered+Double+hydroxides:+Present+and+Future&ots=marudQBzU1&sig=zVuSWEtC5n-_aNl70uD1ajKe1DQ#v=onepage&q&f=false, 159033 060 9,Google Scholar
- Xu ZP, Qing G: Hydrothermal synthesis of layered double hydroxides (LDHs) from mixed MgO and Al2O3, LDH formation mechanism. Chem Mater. 2005, 17 (5): 1055-1062. 10.1021/cm048085g.View ArticleGoogle Scholar
- Liu X, Worden JG, Dai Q, Zou J, Wang J, Huo Q: Monofunctional gold nanoparticles prepared via a noncovalent interaction based on solid-phase modification approach. Small. 2006, 2 (10): 1126-1129. 10.1002/smll.200600162.View ArticleGoogle Scholar
- Berriozabal G, de Miguel YR: Synthesis and characterisation of silica nanoparticles bearing different functional groups obtained via a two-stage method. physica status solidi (C). 2010, 7 (11–12): 2692-2696. 10.1002/pssc.200983837. http://onlinelibrary.wiley.com/doi/10.1002/pssc.200983837/abstract,Google Scholar
- Ajat M, Mohd M, Yusoff K, Hussein MZ: Synthesis of glutamate-zinc-aluminium-layered double hydroxide nanobiocomposites and cell viability study. Current Nanoscience. 2008, 4 (4): 391-396. 10.2174/157341308786306161.View ArticleGoogle Scholar
- Hussein MZ, Al Ali SH, Zainal Z, Hakim MN: Development of antiproliferative nanohybrid Compound with controlled release property using ellagic acid as the active agent. Int J Nanomedicine. 2011, 6: 1373-1383.View ArticleGoogle Scholar
- Al Ali SHH, Al-Qubaisi M, Hussein MZ, Zainal Z, Hakim MN: Preparation of hippurate-zinc layered Hydroxide nanohybrid and its synergistic effect with tamoxifen on HepG2 cell lines. Int J Nanomedicine. 2011, 6: 3099-3111.View ArticleGoogle Scholar
- Hasan S, Al Ali H, Al-Qubaisi M: Controlled-release formulation of antihistamine based on Cetirizine zinc-layered hydroxide nanocomposites and its effect on histamine release from basophilic leukemia (RBL-2H3) cells. Int J Nanomedicine. 2012, 7: 3351-Google Scholar
- Hussein Al Ali SH, Al-Qubaisi M, Hussein MZ, Zainal Z, Hakim MN: Preparation of hippurate-zinc layered hydroxide nanohybrid and its synergistic ezffect with tamoxifen on HepG2 cell lines. Int J Nanomedicine. 2011, 6: 3099-3111.View ArticleGoogle Scholar
- Hussein Al Ali SH, Al-Qubaisi M: Controlled-release formulation of antihistamine based on cetirizine zinc-layered hydroxide nanocomposites and its effect on histamine release from basophilic leukemia (RBL-2H3) cells. Int J Nanomedicine. 2012, 7: 3351-3363.View ArticleGoogle Scholar
- Vasbinder E, Van der Weken G, Van der Heyden Y: Quantitative determination of para-aminosalicylic acid and its degradation product meta-aminophenol in pellets by ion-pair high-performance Liquid chromatography applying the monolithic Chromolith Speed Rod RP-18 column. Biomedical Chromatogr. 2004, 18 (1): 55-63. 10.1002/bmc.292.View ArticleGoogle Scholar
- Newman SP, Jones W: Comparative study of some layered hydroxide salts containing exchangeable interlayer anions. J Solid State Chem. 1999, 148 (1): 26-40. 10.1006/jssc.1999.8330.View ArticleGoogle Scholar
- Xingfu Z, Zhaolin H, Yiqun F, Su C, Weiping D, Nanping X: Microspheric organization of multilayered ZnO nanosheets with hierarchically porous structures. J Physical Chem C. 2008, 112 (31): 11722-11728. 10.1021/jp802619j.View ArticleGoogle Scholar
- Cavani F, Trifiro F, Vaccari A: Hydrotalcite-type anionic clays: preparation, properties and applications. Catalysis today, 11 ( I99 I ) 173-301. 1991, Amsterdam: Elsevier Science Publishers B.V., http://dns2.asia.edu.tw/~ysho/YSHO-English/1000%20CE/PDF/Cat%20Tod11,%20173.pdf,Google Scholar
- Stahlin W, Oswald HR: The crystal structure of zinc hydroxide nitrate, Zn5 (OH) 8 (NO3) 2.2 H2O. Acta Crystallographica Section B. Structural Crystallography and Crystal Chem. 1970, 26 (6): 860-863. 10.1107/S0567740870003230.View ArticleGoogle Scholar
- Akkaya Y, Akyuz S: Infrared and Raman spectra, ab initio calculations vibrational assignment of 4-aminosalicylic acid. Vibrational Spectrosc. 2006, 42 (2): 292-301. 10.1016/j.vibspec.2006.05.011.View ArticleGoogle Scholar
- Rotich MK, Brown ME, Glass BD: Thermal studies on mixtures of aminosalicylic acids with cyclodextrins. J Thermal Anal Calorimetry. 2003, 73 (2): 687-706. 10.1023/A:1025454818939.View ArticleGoogle Scholar
- Husseina MZ, Bahara FA, Yahayac AH: Synthesis and characterization of hippurate-layered double hydroxide nanohybrid and investigation of its release property. J Iran Chem Soc. 2010, 7: 42-51.Google Scholar
- Sing KSW: The use of gas adsorption for the characterization of porous solids. Colloids and Surfaces. 1989, 38 (1): 113-124. 10.1016/0166-6622(89)80148-9.View ArticleGoogle Scholar
- Hussein MZ, Hwa TK: Synthesis and Properties of Layered Organic–inorganic Hybrid Material: Zn-Al Layered Double Hydroxide-dioctyl Sulfosuccinate Nanocomposite. J Nanoparticle Res. 2000, 2 (3): 293-298. 10.1023/A:1010013201391.View ArticleGoogle Scholar
- Tyner KM, Schiffman SR, Giannelis EP: Nanobiohybrids as delivery vehicles for camptothecin. J Controlled Release. 2004, 95 (3): 501-514. 10.1016/j.jconrel.2003.12.027.View ArticleGoogle Scholar
- Dong L, Yan L, Hou WG, Liu SJ: Synthesis and release behavior of composites of camptothecin and layered double hydroxide. J Solid State Chem. 2010, 183 (8): 1811-1816. 10.1016/j.jssc.2010.05.035.View ArticleGoogle Scholar
- Ho YS, Ofomaja AE: Pseudo-second-order model for lead ion sorption from aqueous solutions onto palm kernel fiber. J Hazardous Mater. 2006, 129 (1): 137-142.View ArticleGoogle Scholar
- Kong X, Shi S, Han J, Zhu F, Wei M, Duan X: Preparation of Glycy-l-Tyrosine intercalated layered double hydroxide film and its in vitro release behavior. Chemical Eng J. 2010, 157 (2): 598-604.View ArticleGoogle Scholar
- AlAli SH, Al-Qubaisi M, Hussein MZ, Ismail M, Zainal Z, Hakim MN: Controlled-release formulation of Antihistamine based on cetirizine zinc-layered hydroxide nanocomposites and its effect on histamine release from basophilic leukemia (RBL-2H3) cells. Int J Nanomedicine. 2012, 7: 3351-3363.View ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.