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Table 4 Regression and validation parameters of the established univariate and multivariate methods for determination of TRI and XIP

From: Univariate versus multivariate spectrophotometric data analysis of triamterene and xipamide; a quantitative and qualitative greenly profiled comparative study

Validation parameters

TRI

XIP

D0

PLS

PCR

ANN

MCR-ALS

FSD

PLS

PCR

ANN

MCR-ALS

Linearity range (µg/mL)

2.00–10.00

3.00–7.00

2.00–8.00

2.00–6.00

Correlation coefficient (r)

0.9996

0.9998

0.9995

0.9998

0.9998

0.9992

0.9997

0.9998

0.9996

0.9997

Slope

0.0843

1.015

1.0116

1.0335

0.9996

0.1083

1.0197

1.0457

0.99658

1.0375

Intercept

− 0.035

− 0.181

− 0.169

− 0.3155

− 0.147

-0.092

− 0.1716

− 0.3336

− 0.11378

− 0.3393

RMSEC

–

0.162

0.053

0.156

0.150

–

0.146

0.103

0.136

0.099

RMSEP

–

0.138

0.017

0.104

0.011

–

0.085

0.094

0.144

0.112

Accuracy a (Mean% ± SD)

99.49 ± 1.37

–

–

–

–

100.05 ± 1.28

–

-

-

-

Precisionb

 

Repeatability

1.01

–

–

–

–

1.12

–

–

–

–

Intermediate

0.77

–

–

–

–

1.62

–

–

–

–

LODc

0.22

0.19

0.28

0.14

0.15

0.31

0.20

0.16

0.22

0.16

LOQc

0.66

0.57

0.84

0.42

0.45

0.93

0.60

0.48

0.66

0.48

  1. a Average of three different concentrations repeated three times within the day
  2. b Precision was evaluated by measuring the response of three concentrations of each drug three separate times on the same day (repeatability) and on three different days (intermediate precision)
  3. c LOD and LOQ were calculated from the standard deviation (s) of the response and the slope of the calibration curve (S) according to the following equations: LOD = 3.3(s/S) and
  4. LOQ= 10(s/S)
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BMC Chemistry

ISSN: 2661-801X

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