A green TLC densitometric method for the simultaneous detection and quantification of naphazoline HCl, pheniramine maleate along with three official impurities

Table 3 Regression parameters for determination of the studied drugs by the proposed TLC densitometric method

Parameter	NPZ impurity B	NPZ	PHN	PHN impurity A	PHN impurity B
Range	0.1–10.0 µg band⁻¹	2.0–50.0 µg band⁻¹	10.0–110.0 µg band⁻¹	0.2–50.0 µg band⁻¹	0.2–50.0 µg band⁻¹
Slope	No. 1^a = − 172.85 No. 2^a = 3480.76	No. 1^a = − 13.91 No. 2^a = 1389.10	No. 1^a = − 1.82 No. 2^a = 584.57	No. 1^a = − 9.78 No. 2^a = 943.45	No. 1^a = − 11.64 No. 2^a = 962.46
Intercept	1343.80	3305.64	4730.10	14,708.36	11,166.09
SE of the slope	No. 1^a = 11.13 No. 2^a = 112.36	No. 1^a = 0.54 No. 2^a = 26.87	No. 1^a = 0.10 No. 2^a = 15.50	No. 1^a = 0.45 No. 2^a = 23.73	No. 1^a = 0.59 No. 2^a = 25.15
SE of the Intercept	182.40	249.72	492.06	232.59	202.55
Specificity ^b (mean ± SD)	99.11 ± 1.382	100.72 ± 0.221	100.25 ± 1.054	99.10 ± 1.152	98.89 ± 1.963
Accuracy	99.74	101.15	100.42	99.97	100.99
Repeatability (RSD)	1.28	1.47	1.29	0.74	1.03
Intermediate precision (RSD)	1.77	0.55	1.84	0.96	1.79
Robustness	0.98	0.78	0.84	1.07	1.45
LOD (µg band⁻¹)	0.01	0.60	2.38	0.05	0.06
LOQ (µg band⁻¹)	0.03	1.82	7.21	0.15	0.18
Correlation coefficient (r)	0.999	0.999	0.999	0.999	0.999

^aSlope 1 and 2 are the coefficients of a polynomial regression, A = ax² + bx + c, where A is the integrated peak area, x is the concentration of the drug (μg band⁻¹), a and b are coefficients 1 and 2, respectively, and c is the intercept
^bAverage of determinations in seven laboratory-prepared mixtures

ISSN: 2661-801X