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Porous Cu-MOF nanostructures with anticancer properties prepared by a controllable ultrasound-assisted reverse micelle synthesis of Cu-MOF


The ultrasonic assisted reverse micelle method (UARM) was used to synthesize Cu-MOF from Cu(NO3)2·3H2O and 2,6-pyridine dicarboxylic acid in a 1:1 molar proportion. It has been characterized using FT-IR, XRD, nitrogen adsorption analysis, SEM and TEM–EDX. The morphology of Cu-MOFs was spherical, with an average particle size distribution of less than 100 nm. Using BET analysis, the surface area of Cu-MOF was found to be 284.94 m2/g. The porous morphology of Cu-MOF was also suggested by SEM and TEM analyses. It has anticancer properties against MCF-7 breast cancer cells. Cytotoxicity testing was performed on MCF-7 breast cancer cells using the MTT cell viability assay, and cell proliferation and viability were found to be approximately 24% higher than the control.

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Metal organic frameworks (MOFs) are a type of porous material made up of strong bonds between metal ions and organic linkers [1, 2]. MOFs with a careful constituent selection can have a very high surface area, a large pore volume, and excellent chemical stability. By careful selection of constituents, MOFs can exhibit very high surface area, large pore volume, and excellent chemical stability [3]. Research on synthesis, structures and properties of various MOFs has shown that they are promising materials for many applications, such as energy storage, gas storage, heterogeneous catalysis and sensing [4]. Apart from direct use, MOFs have also been used as support substrates for nanomaterials or as sacrificial templates/precursors for preparation of various functional nanostructures. Ding and co-workers have reviewed MOFs based nanozymes for the treatment of cancer [5,6,7].

Unfortunately, cancer is one of the leading causes of death for millions of people worldwide. Cancer biology has advanced significantly in the last decade, but cancer mortality remains high [8, 9]. Traditional drugs for cancer treatment have limitations such as poor pharmacokinetics, poor biological distribution, and adverse side effects [10]. Chemotherapy is the most commonly used cancer treatment, but it has several drawbacks, the most significant of which are low therapeutic efficiency in the treatment process and side effects on normal cells [8]. Since the 1970s, drug release control in drug delivery has been expanding [10]. Drug delivery systems based on nanoparticles are one of the newer methods in drug delivery systems. Drug delivery systems based on nanoparticles can avoid these issues while also increasing efficiency through targeted drug delivery, controlled release, and drug degradation protection. MOFs, d layered double hydroxides (LDHs), graphene oxide (GO), and magnetite are some examples of popular drug delivery systems that use nanoparticles today [8]. Some cases that have been investigated in the use of MOFs as drug delivery systems include excellent surface, thermal and chemical stability, high pore volumes, regular porosity and easy operation. [8, 10] Linxin et al. have prepared Zn2(EBNB)2(BPY)2.2H2O having drug delivery applications [11]. Numerous biological reports have been reported from organic metal frameworks, including inhibition of human glioma cell growth [12], anticancer activity [13,14,15], and antimicrobial properties [16,17,18]. One of the MOFs with high biological properties is Cu-MOF [19,20,21]. It is worth noting that the true nature of the active sites in many MOFs containing metal ions is saturated with the coordination of organic ligands [22]. Cu-MOF has superior antibacterial and anticancer properties [23, 24]. The bactericidal mechanism of Cu-MOF is due to the diffusion of Cu2+ ions. In addition, the negative charges of lipoproteins are absorbed into the cell wall, they enter the cell and damage the cell wall, alter the function of the enzymes of the cell wall, or create cell wall holes [25].

In this paper, we describe the synthesis of Cu-MOFs using the UARM method, followed by FT-IR, XRD, nitrogen adsorption, SEM, and TEM analyses. Its anticancer activity against MCF-7 breast cancer cells is being studied as well.

Experimental section


Cu (NO3)2·3H2O and 2, 6 pyridine dicarboxylic acid were purchased from Merck. C12H25NaSO4 was purchased from Sigma-Aldrich. Double distilled water (DDW) was used in each experiment.


Cu(NO3)2·3H2O (Merck, 98%) and 2, 6 pyridine dicarboxylic acid (Merck, 99%) are mixed with 1:1 mmol dissolved in 25 mL of DDW during the preparation of the samples using the ultrasonic assisted reverse micelle method. The resulting solution was added to a mixture of 0.077 mmol of sodium lauryl sulphate (C12H25NaSO4) as a surfactant (Sigma, 99%) and 8 mL of C6H14 as solvent. The resulting mixture was then stirred for 1 h at 85 °C. The resulting solution was placed in the ultrasonic device and exposed to ultrasonic irradiation under optimal conditions, which included an ultrasonic duration of 21 min, a power of 175 W, and an ultrasonic temperature of 40 °C. Cu-MOF crystals form after 30 min and are separated by centrifugation and washed with DMF.


The X-ray diffraction (XRD) employed for characterization and determination of the crystalline structure and phases during the synthesis of Cu-MOF. To achieve this aim, a powder X-ray diffractometer (Expert MPD, pananalytical, CuKα = 0.154.6 nm) were used in the range of 2θ = 4 – 30 degree with the step width of 0.05 degree. Scanning electron microscope (SEM, model Em 3200, china KyKy corporation) utilized for investigation of the surface morphology. Fourier transform infrared (FT-IR; SHIMADZU FT8400 spectrometer) with a Bruker- tensor 27 series was utilized for determination of vibrational frequency of the prepared samples in the range of 500 and 4000 cm−1. Porosities, surface area and pore textural characteristics of samples were determined by adsorption/desorption measures (BET, Belsorp mini II) at 77 K in N2 atmosphere. The absorbance was read by spectrophotometer (BioTek Instruments, Inc., Bad Friedrichshall, Germany).

Anticancer activities

Anticancer activity of Cu-MOF was evaluated using MCF-7 breast cancer cells and the MTT cell viability assay according previously reported methods [26, 27]. MCF-7 cells were isolated from the pleural effusion of a 58-year-old woman with metastatic disease. In pellet culture system consisted of control medium including RPMI 1640, 10% FBS and 100 µL of penicillin G/streptomycin mixtures, cells were cultured for a period of 2 weeks. During cell culture, cell passaging was performed by trypsinization and washing was by phosphate-buffered saline. The cells with density of 1.2 × 104 (cells/well) were seeded in 96-well plates and for 24 h incubated in condition of 37 °C and 5% CO2. With concentrations of 5, 10, 20, 40, 80, 120 and 200 μg/mL of Cu-MOF, cells were treated for 24 and 48 h. Then, the medium was then removed and 50 µL/well of MTT solutions (2 mg/mL in PBS) and 150 µL/well of fresh medium were added and incubated for 4 h. Finally, after removing the MTT solutions, to solubilize the formazan crystals, 200 μl of DMSO was added and at 570 nm, the absorbance was read using a spectrophotometer.

Results and discussion

TEM image was used to investigate the topographical study of Cu-MOF, as shown in Fig. 1. It is clear that the morphology of Cu-MOF was porous and spherical and the average particle size distribution is about 50 nm. As a result, the anticancer properties of the sample were investigated. Figure 2 depicts the phase formation and purity of the samples as determined by X-ray diffraction. The sample pattern belongs to Cu-MOF, the peaks marked with a circle at 31°, 37°,43°, 52°, 59°, and 77° for Cu-MOF (JCPDS01-072–0075) [28, 29]. The crystallite size was calculated to be 45 nm using the Debye–Scherrer formula (D = 0.9 λ/β cos θ, where crystal size is expressed by D, X-ray wavelength is expressed by λ and Braggs angle in radians is given by θ is the, and full width at half maximum (FWHM) of the peak in radians is expressed by β.

Fig. 1
figure 1

TEM image of the Cu-MOF and its mapping

Fig. 2
figure 2

The X-ray diffraction pattern of Cu-MOF

The FTIR spectrum of Cu-MOF is depicted in Fig. 3. Surface water is present in the Cu-MOF structure, and as a result, the O–H broad stretching band appears at 3358 cm−1. [30, 31] The weak bands at 2355 and 2099 cm−1 are attributed to COO stretching vibrations present in 2,6-pyridine dicarboxylic acid which serves as organic linker present in Cu-MOF. The bands appeared at 1632 cm−1 is attributed C = O stretching vibrations [16]. The strong absorption bands at 1095 and 1152 corresponds to the asymmetric and symmetric C-O stretching [32, 33]. Absorption bands at 872 and 995 cm−1 are attributed to C-H symmetric and asymmetric stretching vibrations [34]. The peaks observed between 451 and 616 cm−1 are attributed to Cu–O stretching in Cu-MOF [35, 36]. According to the FTIR spectrum, the final structures of Cu-MOF nanostructures are suggested in Fig. 4.

Fig. 3
figure 3

The FTIR spectrum of Cu-MOF

Fig. 4
figure 4

The proposed structures of Cu-MOF

The nitrogen adsorption/desorption results of Cu-MOF structures at 77 K are shown in Fig. 5 It (Fig. 5) depicts isotherms of the type (I) in the IUPAC classification, which is an example of microporous materials. [37] The early isotherm's dramatic increase and high N2 uptake indicate a high proportion of microporous. Furthermore, the amount of microporous is very low because the samples' isotherms in the high pressure region show no obvious hysteresis and tail. [38] In addition, the calculated N2 adsorption/desorption isotherms were related to textural parameters. The surface area of Cu-MOF measured using BET analysis was 284.94 m2/g. As a result, the high surface area and porosity of this sample are determined by N2 uptake by Cu-MOF.

Fig. 5
figure 5

Nitrogen adsorption desorption curve for Cu-MOF at 77 K

Cu-MOF cytotoxicity was tested on MCF-7 breast cancer cells and the results are shown in Fig. 6. Based on the results of Fig. 6, after 48 h with high concentrations of Cu-MOF (200 g/mL), cell proliferation and viability were observed to be approximately 24% higher than the control. The IC50 value for exposure of Cu-MOF at 24 h, 131 μg/mL was obtained and following at 48 h, 109 μg/mL was obtained. Therefore, breast cancer cell survival was dependent on concentrations of Cu-MOF and time of incubation.

Fig. 6
figure 6

Anticancer results of Cu-MOF in MCF-7 breast cancer cell for 24 and 48 h. Data represents mean (n = 3) ± SD


Cu-MOF was prepared using UARM method and it was further characterized by FT-IR, XRD, SEM and nitrogen adsorption/desorption analysis. It has shown reasonably good anticancer activities against MCF-7 breast cancer cells. In summary, the synthesized Cu-MOF exhibited anti-cancer properties against MCF-7 breast cancer with an IC50 value of 109 μg/mL in 48 h and viability about 24% with the highest test concentration (200 μg/mL) was obtained at 48 h.

Availability of data and materials

The datasets used and/or analyzed during the current study available from the corresponding author on request.


  1. James SL. Metal-organic frameworks. Chem Soc Rev. 2003;32:276–88.

    CAS  Article  Google Scholar 

  2. Zhu L, Liang G, Guo C, Xu M, Wang M, Wang C, Zhang Z, Du M. A new strategy for the development of efficient impedimetric tobramycin aptasensors with metallo-covalent organic frameworks (MCOFs). Food Chem. 2022;366: 130575.

    CAS  Article  Google Scholar 

  3. Furukawa H, Cordova KE, O’Keeffe M, Yaghi OM. The chemistry and applications of metal-organic frameworks. Science. 2013.

    Article  PubMed  Google Scholar 

  4. Tansell AJ, Jones CL, Easun TL. MOF the beaten track: unusual structures and uncommon applications of metal–organic frameworks. Chem Cent J. 2017;11:1–16.

    Article  Google Scholar 

  5. Ding SS, He L, Bian XW, Tian G. Metal-organic frameworks-based nanozymes for combined cancer therapy. Nano Today. 2020;35: 100920.

    CAS  Article  Google Scholar 

  6. Ji X, Hou C, Gao Y, Xue Y, Yan Y, Guo X. Metagenomic analysis of gut microbiota modulatory effects of jujube (Ziziphus jujuba Mill.) polysaccharides in a colorectal cancer mouse model. Food Funct. 2020;11:163–73.

    CAS  Article  Google Scholar 

  7. Tang W, Wan S, Yang Z, Teschendorff AE, Zou Q. Tumor origin detection with tissue-specific miRNA and DNA methylation markers. Bioinformatics. 2018;34:398–406.

    CAS  Article  Google Scholar 

  8. Pooresmaeil M, Namazi H. Facile preparation of pH-sensitive chitosan microspheres for delivery of curcumin; characterization, drug release kinetics and evaluation of anticancer activity. Int J Biol Macromol. 2020;162:501–11.

    CAS  Article  Google Scholar 

  9. Yan S, Yan J, Liu D, Li X, Kang Q, You W, Zhang J, Wang L, Tian Z, Lu W. A nano-predator of pathological MDMX construct by clearable supramolecular gold (I)-thiol-peptide complexes achieves safe and potent anti-tumor activity. Theranostics. 2021;11:6833.

    CAS  Article  Google Scholar 

  10. Sun L-L, Li Y-H, Shi H. A ketone functionalized Gd (III)-MOF with low cytotoxicity for anti-cancer drug delivery and inhibiting human liver cancer cells. J Cluster Sci. 2019;30:251–8.

    CAS  Article  Google Scholar 

  11. Linxin D, Song L, Xuehua SJ. The properties of MOF-Zn 2 (EBNB) 2 (BPY) 2· 2H 2 O and its basic study of loading methadone. BMC Chem. 2020;14:1–8.

    Article  Google Scholar 

  12. Hu YJ, Li YM, Zheng K, Zhang WH, Liu YL, Yang H. Construction of a Zn (II)-containing MOF for highly selective detection of picric acid and inhibition of human glioma cell growth. J Mol Str. 2020;1202: 127359.

    CAS  Article  Google Scholar 

  13. Huang J, Pu X-Y, Liu Z, Cao X-Y, Fu J. A 2-fold interpenetrating Zn (II)-MOF: sensitive detection of Fe 3+ ion and anti-cancer activity on breast cancer through high intensity focused ultrasound. J Inorg Organomet Polym Mater. 2021;31:2209–17.

    CAS  Article  Google Scholar 

  14. Yan Z, Li X, Fan Q, Bai H, Wu S, Zhang ZF, Pan L. A water-stable and biofriendly Zn-MOF with pyrazine decorated pores as 5-Fu delivery system to induce human ovarian cancer cells apoptosis and abrogate their growth. J Mol Str. 2020;1204: 127477.

    CAS  Article  Google Scholar 

  15. Song B-H, Ding X, Zhang Z-F, An G-F. Efficient drug delivery of 5-fluorouracil by a biocompatible Zn-metal–organic framework nanostructure and anti-liver cancer activity study. J Iran Chem Soc. 2019;16:333–40.

    CAS  Article  Google Scholar 

  16. Akbarzadeh F, Motaghi M, Chauhan NP, Sargazi G. A novel synthesis of new antibacterial nanostructures based on Zn-MOF compound: design, characterization and a high performance application. Heliyon. 2020;6: e03231.

    Article  Google Scholar 

  17. Restrepo J, Serroukh Z, Santiago-Morales J, Aguado S, Gómez-Sal P, Mosquera ME, Rosal R. An antibacterial Zn–MOF with hydrazinebenzoate linkers. Eur J Inorg Chem. 2017;2017:574–80.

    CAS  Article  Google Scholar 

  18. Gwon K, Han I, Lee S, Kim Y, Lee DN. Novel metal-organic framework-based photocrosslinked hydrogel system for efficient antibacterial applications. ACS Appl Mater Interfaces. 2020;12:20234–42.

    CAS  Article  Google Scholar 

  19. Chen D, Li B, Jiang L, Li Y, Yang Y, Luo Z, Wang J. Pristine Cu-MOF induces mitotic catastrophe and alterations of gene expression and cytoskeleton in ovarian cancer cells. ACS Appl Bio Mater. 2020;3:4081–94.

    CAS  Article  Google Scholar 

  20. Xuan Z, Zhan Y, Song G, Liu B. Two Cu (II) co-ordination polymers: anti-cancer activity on melanoma by reducing cancer cell proliferation, migration, and invasion ability, inorganic and nano-metal. Chemistry. 2021;51:239–45.

    CAS  Google Scholar 

  21. Azizabadi O, Akbarzadeh F, Danshina S, Chauhan NP, Sargazi G. An efficient ultrasonic assisted reverse micelle synthesis route for Fe3O4@ Cu-MOF/core-shell nanostructures and its antibacterial activities. J Solid State Chem. 2021;294: 121897.

    CAS  Article  Google Scholar 

  22. Zou R-Q, Sakurai H, Han S, Zhong R-Q, Xu Q. Probing the lewis acid sites and CO catalytic oxidation activity of the porous metal−organic polymer [Cu (5-methylisophthalate)]. J Am Chem Soc. 2007;129:8402–3.

    CAS  Article  Google Scholar 

  23. Pires AS, Batista J, Murtinho D, Nogueira C, Karamysheva A, Luísa Ramos M, Milne BF, Tavares NT, Gonçalves J, Gonçalves AC. Synthesis, characterization and evaluation of the antibacterial and antitumor activity of HalogenatedSalen Copper (II) complexes derived from camphoric acid. Appl Organomet Chem. 2020.

    Article  Google Scholar 

  24. Gu L, Wang P, Zhong Q, Deng Y, Xie J, Liu F, Xiao F, Zheng S, Chen Y, Wang G. Copper salt-catalyzed formation of a novel series of triazole–spirodienone conjugates with potent anticancer activity. RSC Adv. 2017;7:9412–6.

    CAS  Article  Google Scholar 

  25. Wang Y, Wang F. Post-translational modifications of deubiquitinating enzymes: expanding the ubiquitin code. Front Pharmacol. 2021;12:1434.

    Google Scholar 

  26. Moghaddam-manesh M, Beyzaei H, Majd MH, Hosseinzadegan S, Ghazvini K. Investigation and comparison of biological effects of regioselectively synthesized thiazole derivatives. J Heterocycl Chem. 2021.

    Article  Google Scholar 

  27. Ji P, Wang L, Wang S, Zhang Y, Qi X, Tao J, Wu Z. Hyaluronic acid-coated metal-organic frameworks benefit the ROS-mediated apoptosis and amplified anticancer activity of artesunate. J Drug Target. 2020;28:1096–109.

    CAS  Article  Google Scholar 

  28. Süsse PJ. Verfeinerung der kristallstruktur des malachits, Cu2 (OH) 2CO3. Acta Crystallogr. 1967;22:146–51.

    Article  Google Scholar 

  29. Riccò R, Linder-Patton O, Sumida K, Styles MJ, Liang K, Amenitsch H, Doonan CJ, Falcaro PJ. Conversion of copper carbonate into a metal–organic framework. Chem Mater. 2018;30:5630–8.

    Article  Google Scholar 

  30. Lin J, Wang D, Chen D, Ge Q, Ping G, Fan M, Qin L, Shu K. Preparation and enhanced photocatalytic performance of one-dimensional ZnO nanorods. Environ Prog Sustain Energy. 2015;34:74–80.

    CAS  Article  Google Scholar 

  31. Kaur R, Kaur A, Umar A, Anderson WA, Kansal SK. Metal organic framework (MOF) porous octahedral nanocrystals of Cu-BTC: Synthesis, properties and enhanced adsorption properties. Mater Res Bull. 2019;109:124–33.

    CAS  Article  Google Scholar 

  32. Lin S, Song Z, Che G, Ren A, Li P, Liu C, Zhang J. Adsorption behavior of metal–organic frameworks for methylene blue from aqueous solution. Microporous Mesoporous Mater. 2014;193:27–34.

    CAS  Article  Google Scholar 

  33. Azad FN, Ghaedi M, Dashtian K, Hajati S, Pezeshkpour V. Ultrasonically assisted hydrothermal synthesis of activated carbon–HKUST-1-MOF hybrid for efficient simultaneous ultrasound-assisted removal of ternary organic dyes and antibacterial investigation: Taguchi optimization. Ultrason Sonochem. 2016;31:383–93.

    CAS  Article  Google Scholar 

  34. Ji X, Cheng Y, Tian J, Zhang S, Jing Y, Shi M. Structural characterization of polysaccharide from jujube (Ziziphus jujuba Mill.) fruit. Chem Biol Technol Agric. 2021;8:1–7.

    Article  Google Scholar 

  35. Stehfest K, Boese M, Kerns G, Piry A, Wilhelm C. Fourier transform infrared spectroscopy as a new tool to determine rosmarinic acid in situ. J Plant Physiol. 2004;161:151–6.

    CAS  Article  Google Scholar 

  36. Mai NXD, Yoon J, Kim JH, Kim IT, Son HB, Bae J, Hur J. Hybrid hydrogel and aerogel membranes based on chitosan/prussian blue for photo-fenton-based wastewater treatment using sunlight. Sci Adv Mater. 2017;9:1484–7.

    CAS  Article  Google Scholar 

  37. Rouquerol J, Rouquerol F, Llewellyn P, Maurin G, Sing KS. Adsorption by powders and porous solids: principles, methodology and applications. Cambridge: Academic press; 2013.

    Google Scholar 

  38. Javanbakht S, Pooresmaeil M, Namazi H. Green one-pot synthesis of carboxymethylcellulose/Zn-based metal-organic framework/graphene oxide bio-nanocomposite as a nanocarrier for drug delivery system. Carbohyd Polym. 2019;208:294–301.

    CAS  Article  Google Scholar 

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The authors would like to acknowledge from the Bam University of Medical Sciences.


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Authors and Affiliations



RA-S: methodology, software, visualization. MZ: methodology, software, writing—original visualization. MM-M: methodology, software, visualization. SH: writing—reviewing and editing investigation. PK: writing—reviewing and editing investigation. NPSC: writing—original, visualization, writing—reviewing and editing investigation. GS: conceptualization, methodology, software, writing—original, visualization, writing—reviewing and editing investigation, data curation, validation, supervision. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Ghasem Sargazi.

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We confirm all relevant ethical guidelines have been followed, and any necessary IRB and/or ethics committee approvals have been obtained. All methods were used in accordance with relevant guidelines and regulations. Also, we confirmed that all experimental protocols were approved by the Ethics Licensing Committee of the Bam University of Medical Sciences (no. 06 on 17/03/2021). MCF-7 cells were isolated from the pleural effusion of a 58-year-old woman with metastatic disease. In addition, informed consent was obtained from all study participants.

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Akhavan-Sigari, R., Zeraati, M., Moghaddam-Manesh, M. et al. Porous Cu-MOF nanostructures with anticancer properties prepared by a controllable ultrasound-assisted reverse micelle synthesis of Cu-MOF. BMC Chemistry 16, 10 (2022).

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  • Cu-MOF
  • Characterization
  • Cell viability
  • Anticancer