- Research article
- Open Access
Evaluation of structurally different benzimidazoles as priming agents, plant defence activators and growth enhancers in wheat
BMC Chemistryvolume 13, Article number: 29 (2019)
Priming is a valuable, facile and well-established technique used to enhance seed quality to achieve rapid germination, establishment of stress resistance and improvement of crop yields. Different natural and synthetic priming agents have been used for better crop performance and abiotic stress management. In this study, four different benzimidazoles were selected as priming agents and their comparative effects were evaluated on different biochemical attributes including total soluble protein, total oxidant status, MDA contents, antioxidant enzymes (SOD, POD) and hydrolytic enzymes (protease, estrases) compared to control. Treatments with 2-thio-1-H-benzimidazole reduced total soluble proteins and increased total oxidant status significantly but no considerable effect was observed on other parameters. Priming with 2-(4-chlorophenyl)-1-H-benzimidazole considerably increased the total oxidant status and a little improvement was observed in total soluble proteins. Seeds primed with 1-H-benzimidazole showed a noticeable decrease in the protease activity while all other priming treatments were unable to induce any detectable change compared to control. The treatment with 2-(4-methoxyphenyl)-1-H-benzimidazole induced maximum reduction in MDA contents and POD activity. Moreover, all benzimidazole priming treatments reduced mean germination time, increased germination percentage and germination rate of wheat seeds.
Due to rising global population, it has been estimated that the demand for wheat is going to be doubled in 2050 . To satisfy these rising wheat demands, farmers are supposed to boost crop yields by adopting new farming strategies. In this context, enhanced seed qualities has become priority requirements to achieve uniform and rapid seedling emergence for better crop performance and finally increased yield . Seed quality is enhanced by employing facile, easily practicable and well established treatment called priming . As a result of priming treatments, germination rate increases with the development of high level stress tolerance which enhances crop yields . In fact, priming induces pre-germinative metabolism to various level in seeds depending upon their species, physiology and morphology . These specific metabolic changes trigger ATP production, de-novo synthesis of proteins and nucleic acids, activation of antioxidant enzymes and DNA repair, accumulations of phospholipids and sterols [6, 7]. The activation of these cellular mechanisms protect genome integrity, ensure rapid germination with fast seedling emergence thus help to provide high crop yields .
Around the globe wheat is the major cereal crop fulfilling almost half of the protein requirements and feeds at least one-third world population. Often wheat crop productivity is limited by slow germination rate, reduced seedling vigor, slow growth and development rates under normal and stress conditions . Under such situations, various natural and synthetic chemicals have been used as priming agents for various crops including wheat. Chemical priming offers effective opportunities for crop stress managements as it induces significant tolerance against a range of abiotic stresses . On-farm priming of wheat seeds with ascorbic acid, salicylic acid, auxins, H2O2, polyethylene glycol, kinetin and GA3 etc. has been reported to improve aforementioned germination, seedling growth, non-enzymatic and enzymatic antioxidants related attributes leading to high grain yield .
The benzimidazole and its derivatives are exceptional structural motif of wide interest exhibiting a broad spectrum of applications across a range of scientific disciplines [11,12,13]. The benzimidazole nucleus with varied substituents has proved as a privileged moiety with diverse potential of clinical and biological activities including antiviral, antibacterial, anti-tumor, anti-hypertensive, anti-diabetic and anti-HIV etc. [14, 15]. Compounds incorporating benzimidazole have also been used as agrochemicals with fungicidic and plant growth regulating properties . Further, they provide protection and insulate plants against various environmental stresses . Mangnucka et al. treated rye grains with 10 ppm of carbendazim and benomyl before they were allowed to germinate for 5 days . These benzimiazole-based fungicides greatly affected the biosynthesis of resorcinol and fresh and dry biomass of seedlings under thermal and light growth conditions. Seed treatments with Ambiol®, a known benzimidazole-based antioxidant increased germination, enhanced growth and improved stress tolerance in seedlings of many species [19,20,21]. Tomato seed treatments with Ambiol induced positive effects on germination, growth and seedling development which were passed-on to next generation. Vital parameters like photosynthesis, leaf area, percent germination, root mass and shoot mass were considerably improved in parents as well as in progeny .
In this study four different benzimidazoles were selected as wheat seed priming agents and their effects on biochemical attributes were evaluated. The subsequent sections do explain the comparative effects of these benzimidazoles on vital biochemical and germination parameters.
Materials and methods
Seed collection and priming
For this priming study, the spring wheat (Triticumaestivum L. cv. GLAXY-2013) seeds were obtained from Wheat Section, Nuclear Institute of Agriculture and Biology (NIAB), Faisalabad, Pakistan. Wheat seed priming was achieved by soaking them in aerated solutions of four different benzimidazoles with 20 and 30 ppm concentrations for 8 h. Afterwards, they were washed and dried under shade at 26 ± 2 °C until they gained original weight. Separately, seeds were soaked in distilled water for 8 h to achieve hydro-priming. Untreated or non-primed seeds were used as control for comparison in biochemical analyses and germination studies.
Biochemical analysis and germination studies
Different biochemical parameters were analyzed in primed, hydro-primed and non-primed wheat seeds to evaluate the effects of benzimidazole priming treatments. According to well-established methods for estimation and extraction of enzymes and other biochemical parameters, hydro-primed, primed and non-primed seeds were grounded using 50 mM potassium phosphate buffer with pH 7.4. At 4 °C, the grounded material was put on centrifugation at 15,000×g for 20 min and the supernatant was used for quantification studies of different enzymes. The method described by Bradford was followed for protein estimation in seed samples . Total oxidant status was determined by following the method presented by Erel et al. . This method estimates the presence of oxidants which oxidize Fe+2 to Fe+3. The method presented by Giannopolitis and Ries was followed with little modification to determine superoxide dismutase (SOD) activities . The method initially presented by Heath and Packer and then modified by Dhindsa et al. and Zhang and Kirkham was used to determine malondialdehyde (MDA) contents [27,28,29]. The method of Drapeau was followed for protease activity determination . The method developed by Chance and Maehly was employed for the determination of peroxidase (POD) activities . The enzyme activities were expressed on seed weight basis. According to the methods of Van Asperen , the α-naphthyl acetate and β-naphthyl acetate were used as substrates for the determination of α-esterases and β-esterases .
Germination potential of the primed and control wheat seeds was estimated. To test seed germination and seedling vigor under osmotic stress, four replicates of 25 seeds were germinated in 12 cm diameter petri dishes at 25 °C. A seed was scored as germinated when coleoptile and radicle lengths reached 2–3 mm. Counts of germinating seeds were made twice a day at different time intervals (20, 28, 44, 52, 68, 76, 92 and 100 h), starting on the first day of imbibition, and terminated when maximum germination was achieved. Final germination percentage was measured according to following formula (Fig. 2).
Mean germination time (MGT) was calculated as following ,
The recorded data was analyzed statistically by applying descriptive statistics. The significance between means was measured using Tucky’s test at 5% probability level using XL-STAT. Values presented are mean ± SD with different alphabets differ significantly from each other.
Results and discussions
Changes in the total soluble protein contents in non-primed, hydro-primed and benzimidazole primed wheat seeds were measured (Fig. 3). A noticeable improvement in the protein contents was observed in the seeds primed with 30 ppm of both 1-H-benzimidazole and 2-(4-chlorophenyl)-1-H-benzimidazole. While priming with 20 ppm of 2-thio-1-H-benzimidazole reduced total soluble proteins to some extent compared to control. However, all other treatments showed no apparent difference in protein contents compared to control. It may be suggested that the priming with benzimidazoles did not interrupt the cellular pathways or related enzymes involved in the biosynthesis of proteins. Jafar et al. reported an increase in total soluble proteins when wheat seeds were primed with salicylicate, kinetin, CaCl2 and ascorbate . Similarly, Bajwa et al. also reported an increase in total soluble proteins when benzyl amino purine was used as a priming agent for wheat seeds .
Effects of different benzimidazole seed priming treatments on total oxidant status in wheat seeds were evaluated (Fig. 4). Total oxidant status increased remarkably in seeds primed with 20 ppm 2-thio-1-H-benzimidazole and 30 ppm 2-(4-chlorophenyl)-1-H-benzimidazole as compared to untreated control seeds. While a noticeable decrease in total oxidant status was observed as a result of 20 ppm 1-H-benzimidazole and hydro-priming. The oxidants were long considered as damaging species for germinating seeds. Recent studies have confirmed their well-established functions in cell signalling, regulation of gene expressions and mobilization of reserves during seed germination . In germinating seeds the metabolically active compartments like mitochondria (for respiratory activities), plasma membrane (by NADPH oxidase) glyoxysomes (for lipid catabolism), peroxisomes (for purine catabolism) become main source of oxidants production. Strong increase in respiratory activities with enhanced production of oxidants are associated with germination [40, 41]. The aforementioned benzimiazole treatments which increased total oxidants significantly might have accelerated the metabolic activities to boost seed germination. It has also been confirmed from the fast germination rate during the first 24 h as shown in (Fig. 14).
During this wheat seed priming study, the level of lipid peroxidation in seeds was measured in terms of MDA contents (Fig. 5) [42, 43]. Priming with 20 ppm of 1-H-benzaimidazole, 2-(4-chlorophenyl)-1-H-benzimidazole and 2-(4-methoxyphenyl)-1-H-benzimidazole showed no observable difference in MDA contents as compared to control. Whereas, all other treatments showed a significant reduction in the MDA contents as compared to control. The treatment with 30 ppm 2-(4-methoxyphenyl)-1-H-benzimidazole induced maximum reduction in MDA contents. The MDA contents are considered as indicator of lipid peroxidation caused by reactive oxygen species (ROS).
The ROS are toxic by-products of aerobic metabolism and results in oxidative stress. The oxidative stress cases destruction of biomolecules like lipid, proteins, DNA and also inactivates antioxidant enzymes . Reduction in MDA level represents low levels of oxidative stress while high levels of MDA suggest overproduction of fatal free radicals [45, 46]. It may be concluded that seed priming with 30 ppm 2-(4-methoxyphenyl)-1-H-benzimidazole reduced ROS levels and oxidative stress in wheat. Wheat seed priming with polyethylene glycol has been reported to reduce MDA contents . Recently, priming treatments with mercapto-triazoles also reduced MDA content in wheat seeds representing a reduction in oxidative stress .
The changes in protease activity in hydro-primed, benzimidazole primed and control wheat seed were also examined (Fig. 6). Seeds primed with 30 ppm of 1-H-benzimidazole showed a perceptible decrease in the protease activity while all other priming treatments were unable to induce any detectable change compared to control. No change in protease activity suggests that the proteins are in un-hydrolysed form in seeds primed with benzimidazoles. It is also confirmed by the unchanged contents of the total soluble proteins shown in Fig. 2 .
Treatment with 20 ppm 2-thio-1-H-benzimidazole and 2-(4-methoxyphenyl)-1-H-benzimidazole induced an observable decrease in SOD compared to control. Priming with both levels of 1-H-benzimidazole and 30 ppm of both 2-thio-1-H-benzimidazole and 2-(4-chlorophenyl)-1-H-benzimidazole presented maximum decrease in SOD activity compared to control (Fig. 7). Previously, it has been reported that the different combinations of chemical and hormonal treatments increased SOD activity in wheat seeds . Wheat seed priming with chitosan and sodium nitroprusside (SNP) have also been reported to increase SOD activity [51, 52]. The SOD acts as a first line of defence against oxidative stress as these metalloenzymes catalyse dismutation of superoxide radicals to oxygen and hydrogen peroxide .
A significant decrease in POD activity was observed in seeds primed with 20 ppm 1-H-benzimidazole, 20 and 30 ppm 2-(4-chlorophenyl)-1-H-benzimidazole compared to control. Also, priming with 20 ppm 2-(4-methoxyphenyl)-1-H-benzimidazole decreased the POD (Fig. 8). However, no perceptible change in POD was recorded as a result of treatments with 2-thio-1-H-benzimidazole. The POD helps in scavenging reactive oxygen species which otherwise could cause oxidative injury . The down regulation of POD suggests its fewer requirements with parallel low production of ROS in primed seeds. From the decreased SOD and POD levels in primed seeds, it could be presumed that benzimidazole treatments have protected the wheat seeds from oxidative stress. In our previous studies, a decrease in POD activity was also recorded when wheat seeds were primed with 10, 15 and 20 ppm of four structurally different triazoles .
Except 20 ppm 2-(4-chlorophenyl)-1-H-benzimidazole all other priming treatments significantly increased the esterase activity compared to control (Fig. 9). The maximum boost in esterase activity was induced as a result of priming with 20 ppm of both 1-H-benzimidazole and 2-thio-1-H-benzimidazole. The treatment with 20 ppm 2-(4-methoxyphenyl)-1-H-benzimidazole and 30 ppm of both 1-H- benzimidazole and 2-(4-chlorophenyl)-1-H-benzimidazole increased esterase activity equivalent to hydro-priming. The increased activity of estrases represents accelerated metabolic processes in germinating wheat seeds. Indirectly, it has also been confirmed from high level of total oxidants and low contents of MDA. Increase in esterase activity was also observed when wheat seeds were primed with SNP as reported by Hameed et al. .
Further, the benzimidazole priming effects on wheat seed germination parameters were also evaluated. All priming treatments showed no significant effect on germination percentage of wheat seeds as compared to control (Fig. 10). However, preconditioning of tomato seeds with Ambiol were reported to increase germination percentage by 12.4% . Other literature reports suggests that wheat seed priming with triazolic compounds, hormones and sodium nitroprusside induced an increase in percentage germination [48, 52, 55].
All benzimidazole treatments decreased the mean germination time (MGT) of wheat seeds as compared to control seeds (Fig. 11). Hydro-priming also effectively decreased the MGT of seeds. The shortest mean germination time with most rapid germination was observed in seeds treated with 20 ppm of 2-thio-1-H-benzimidazole and proved the best priming treatment in this regard. It has been reported that wheat seed priming with SNP also reduced GMT . Preconditioning of tomato seeds with Ambiol also significantly reduced MGT .
The effects of benzimidazole priming on wheat seed germination index were also evaluated (Fig. 12). The results showed that benzimidazole treatments increased the germination index of wheat seeds. A significant increase in germination index was induced by 20 ppm 2-(4-methoxyphenyl)-1-H-benzimidazole priming treatment. Wheat seed priming with differently substituted triazoles also reported to improve germination rate and germination index .
Effects of benzimidazole priming were also evaluated on wheat seed germination energy (Fig. 13). All priming treatments showed no significance effect on germination energy as compared to control.
Effect of benzimidazole treatments on germination rate was observed. All benzimidazole treatments induced early germination during first 24 h when the control seeds were not germinating at all (Fig. 14). Previously, it has also been observed that priming with triazolic compounds, hormones and SNP increased germination rate in wheat seed [48, 52].
In conclusion, differently substituted benzimidazoles induced different effects on each biochemical parameters. Treatments with 20 ppm 2-thio-1-H-benzimidazole reduced total soluble proteins and increased total oxidant status significantly. Priming with 30 ppm 2-(4-chlorophenyl)-1-H-benzimidazole considerably increased total oxidant status and a little improvement was observed in total soluble proteins whereas treatment with its 20 ppm did not affect esterase activity. Seeds primed with 30 ppm of 1-H-benzimidazole showed a perceptible decrease in the protease activity while all other priming treatments were unable to induce any detectable change compared to control. The treatment with 30 ppm 2-(4-methoxyphenyl)-1-H-benzimidazole induced maximum reduction in MDA contents and priming with its 20 ppm decreased POD activity. All benzimidazole priming treatments reduced mean germination time, increased germination percentage and germination rate of wheat seeds and have numerous potential to be used as germination enhances under normal and stressed conditions.
reactive oxygen species
mean germination time
Weigand C (2011) Wheat import projections towards 20. US Wheat Associates, USA, pp 1–14
Osburn R, Schroth M (1988) Effect of osmopriming sugar beet seed on exudation and subsequent damping-off caused by Pythium ultimum. Phytopathology. 78(9):1246–1250
Paparella S, Araújo S, Rossi G, Wijayasinghe M, Carbonera D, Balestrazzi A (2015) Seed priming: state of the art and new perspectives. Plant Cell Rep 34(8):1281–1293
Jisha K, Vijayakumari K, Puthur JT (2013) Seed priming for abiotic stress tolerance: an overview. Acta Physiol Plant 35(5):1381–1396
Bray C (1995) Biochemical processes during the osmopriming of seeds. Seed development and germination. Marcel Dekker, New York, pp 767–789
Ventura L, Donà M, Macovei A, Carbonera D, Buttafava A, Mondoni A et al (2012) Understanding the molecular pathways associated with seed vigor. Plant Physiol Biochem 60:196–206
Waterworth WM, Drury GE, Bray CM, West CE (2011) Repairing breaks in the plant genome: the importance of keeping it together. New Phytol 192(4):805–822
Karssen CM, Haigh A, Van der Toorn P, Weges R (1989) Physiological mechanisms involved in seed priming Recent advances in the development and germination of seeds. Springer, Berlin, pp 269–280
Boyer JS (1982) Plant productivity and environment. Science 218(4571):443–448
Savvides A, Ali S, Tester M, Fotopoulos V (2016) Chemical priming of plants against multiple abiotic stresses: mission possible? Trends Plant Sci 21(4):329–340
Barot KP, Nikolova S, Ivanov I, Ghate MD (2013) Novel research strategies of benzimidazole derivatives: a review. Mini Rev Med Chem 13(10):1421–1447
Davidse LC (1986) Benzimidazole fungicides: mechanism of action and biological impact. Annu Rev Phytopathol 24(1):43–65
Demartino RN (1984) Comfort properties of polybenzimidazole fiber. Text Res J 54(8):516–521
Ajani OO, Aderohunmu DV, Ikpo CO, Adedapo AE, Olanrewaju IO (2016) Functionalized benzimidazole scaffolds: privileged heterocycle for drug design in therapeutic medicine. Arch Pharm 349(7):475–506
Bansal Y, Silakari O (2012) The therapeutic journey of benzimidazoles: a review. Bioorg Med Chem 20(21):6208–6236
Benzimidazoles (1999) In: Roberts TR, Hutson DH, editors. Metabolic pathways of agrochemicals: part 2: insecticides and fungicides: The Royal Society of Chemistry. pp 1105–1112
Baum JS, Chen TM (1986) Plant growth and development modification using 2-(2-oxycarbonylphenyl)benzimidazole derivatives. Google Patents
Magnucka EG, Suzuki Y, Pietr SJ, Kozubek A, Zarnowski R (2007) Action of benzimidazole fungicides on resorcinolic lipid metabolism in rye seedlings depends on thermal and light growth conditions. Pestic Biochem Physiol 88(2):219–225
Borsos-Matovina V. The effect of Ambiol on growth and stress tolerance of Pinus banksiana and Picea mariana seedlings: National Library of Canada=Bibliothèque nationale du Canada; 1999
Rajasekaran LR, Blake TJ (2002) Seed pre-treatment using a derivative of 5-hydroxybenzimidazole (AMBIOL) pre-acclimates carrot seedlings to drought. Can J Plant Sci 82(1):195–202
Voronina S, Zhizhina G, Lozovskaya E (2001) Biophysical aspects of the action of plant growth regulators ambiol and fonk. Biophysics. 46(1):32–36
MacDonald MT, Lada RR, Robinson AR, Hoyle J (2010) The benefits of Ambiol® in promoting germination, growth, and drought tolerance can be passed on to next-generation tomato seedlings. J Plant Growth Regul 29(3):357–365
Hwu JR, Singha R, Hong SC, Chang YH, Das AR, Vliegen I et al (2008) Synthesis of new benzimidazole–coumarin conjugates as anti-hepatitis C virus agents. Antiviral Res 77(2):157–162
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1–2):248–254
Erel O (2005) A new automated colorimetric method for measuring total oxidant status. Clin Biochem 38(12):1103–1111
Giannopolitis CN, Ries SK (1977) Superoxide dismutases I. Occurrence in higher plants. Plant Physiol 59(2):309–314
Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125(1):189–198
Dhindsa RS, Plumb-Dhindsa P, Thorpe TA (1981) Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J Exp Bot 32(1):93–101
Zhang J, Kirkham M (1994) Drought-stress-induced changes in activities of superoxide dismutase, catalase, and peroxidase in wheat species. Plant Cell Physiol 35(5):785–791
Drapeau GR (1977) Cleavage at glutamic acid with staphylococcal protease. Methods Enzymol 47:189–191
Chance B, Maehly A (1955) Assay of catalases and peroxidases. Methods Enzymol 2:764–775
Anonymous (1990) Association of official seed analysts (AOSA). Rules for testing seeds. Seed Sci Technol 12:1–112
Van Asperen K (1962) A study of housefly esterases by means of a sensitive colorimetric method. J Insect Physiol 8(4):401–416
Ellis R, Roberts E (1981) The quantification of ageing and survival in orthodox seeds. Seed Sci Technol (Netherlands) 2:373–409
Aosa I (1983) Seed vigor testing handbook. Assoc Off Seed Anal Contri. 32:32
Ruan S, Xue Q, Tylkowska K (2002) The influence of priming on germination of rice (Oryza sativa L) seeds and seedling emergence and performance in flooded soil. Seed Sci Technol 30(1):61–67
Jafar MZ, Farooq M, Cheema MA, Afzal I, Basra SMA, Wahid MA et al (2012) Improving the performance of wheat by seed priming under saline conditions. J Agron Crop Sci 198(1):38–45
Bajwa AA, Farooq M (2017) Seed priming with sorghum water extract and benzyl amino purine along with surfactant improves germination metabolism and early seedling growth of wheat. Arch Agron Soil Sci 63(3):319–329
Bailly C, Kranner I (2011) Analyses of reactive oxygen species and antioxidants in relation to seed longevity and germination. Seed Dormancy. Springer, Berlin, pp 343–367
El-Maarouf-Bouteau H, Bailly C (2008) Oxidative signaling in seed germination and dormancy. Plant Signal Behav 3(3):175–182
Gomes M, Garcia Q (2013) Reactive oxygen species and seed germination. Biologia. 68(3):351–357
Gawel S, Wardas M, Niedworok E, Wardas P (2004) Malondialdehyde (MDA) as a lipid peroxidation marker. Wiadomosci lekarskie (Warsaw, Poland: 1960) 57(9–10):453–455
Labudda M (2013) Lipid peroxidation as a biochemical marker for oxidative stress during drought. An effective tool for plant breeding. E-wydawnictwo, Poland. http://www.e-wydawnictwo.eu/Document/DocumentPreview/3342
Das K, Roychoudhury A (2014) Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants. Front Environ Sci 2:53
Shulaev V, Oliver DJ (2006) Metabolic and proteomic markers for oxidative stress new tools for reactive oxygen species research. Plant Physiol 141(2):367–372
Sharma P, Jha AB, Dubey RS, Pessarakli M (2012) Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. J Bot 2012:26
Hameed A, Sheikh M, Hameed A, Farooq T, Basra S, Jamil A (2014) Chitosan seed priming improves seed germination and seedling growth in wheat (Triticum aestivum L.) under osmotic stress induced by polyethylene glycol. Philipp Agric Sci 97(3):294–299
Hameed A, Hameed A, Ahmad M, Saqlain T, Farooq T, Aslam S et al (2016) Triazole priming improves germination potential, antioxidant levels and reduces oxidative damage in wheat seeds. Philipp Agric Sci 99(2):282–296
Palma JM, Sandalio LM, Javier Corpas F, Romero-Puertas MC, McCarthy I, del Río LA (2002) Plant proteases, protein degradation, and oxidative stress: role of peroxisomes. Plant Physiol Biochem 40(6):521–530
Hameed A (2012) Investigation of antioxidant enzymes and biochemical changes in the wheat seeds (freed) induced by different pre-sowing treatments. World Appl Sci J 18:31–36
Hameed A, Sheikh M, Hameed A, Farooq T, Basra S, Jamil A (2013) Chitosan priming enhances the seed germination, antioxidants, hydrolytic enzymes, soluble proteins and sugars in wheat seeds. Agrochimica. 57(2):97–110
Hameed A, Farooq T, Basra S, Sheikh M, Ibrahim M (2015) Wheat seed germination, antioxidant enzymes and biochemical enhancements by sodium nitroprusside priming. Agrochimica. 59(2):93–107
Bowler C, Montagu M, Inze D (1992) Superoxide dismutase and stress tolerance. Annu Rev Plant Biol 43(1):83–116
Vicuna D (2005) The role of peroxidases in the development of plants and their responses to abiotic stresses. Doctoral thesis. Dublin Institute of Technology. https://doi.org/10.21427/D7CW2B
Ulfat A, Majid SA, Hameed A (2017) Hormonal seed priming improves wheat (triticum aestivum l.) field performance under drought and non-stress conditions. Pak J Bot 49(4):1239–1253
AH1 (proposed the project and explained biochemical analyses), AH2 (supervised the priming and biochemical studies), TF (overall supervision and manuscript write-up), RN (interpreted the antioxidant activities), SJ (interpreted hydrolytic enzyme studies and statistical analyses), SB (enzyme studies and proof reading), AA (performed priming studies and acquisition of data), TG (critical proof reading), MA (synthesized the selected benzimidazoles). All authors read and approved the final manuscript.
The authors thankfully acknowledged the Nuclear Institute of Agriculture and Biology (NIAB), Faisalabad, Pakistan for provision of excellent lab facilities for smooth execution of this research work.
The authors declare that they have any competing interests.
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