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Hemostatic chemical constituents from natural medicine Toddalia asiatica root bark by LC-ESI Q-TOF MSE
Chemistry Central Journal volume 11, Article number: 55 (2017)
Toddalia asiatica root bark as an effective hemostatic natural medicine or Chinese materia medica was applied in China for long history, its complex drug action mechanisms and unclear substance basis have been constraining the development of this drug.
An intelligentized strategy by LC-ESI Q-TOF MSE was presented in this study for rapid identification of hemostatic chemical constituents from this natural medicine. Chromatographic separation was performed on a C18 column (150 mm × 2.1 mm, 1.8 μm), the MSE data in both negative and positive ion modes were acquired to record the high-accuracy MS and MS/MS data of all precursor ions. To reduce the false positive identifications, structural confirmation was conducted by comparison with the isolated reference standards (tR and MS, MS/MS data) or matching with natural product databases. Bioassay-guided fractionation of the extract of T. asiatica root bark was also carried out.
As a consequence, 31 natural compounds in T. asiatica root bark got putatively characterized. There were four main coumarins, isopimpinellin (Cp.23), pimpinellin (Cp.24), coumurrayin (Cp.30) and phellopterin (Cp.34) isolated and identified from T. asiatica root bark. The present study provided candidate strategy that helps to effectively identify the primary natural compounds of TCM or other complex natural medicines, and then promote development and application of natural medicines and their medicinal resources.
Chinese materia medica as an integral part of traditional Chinese medicine (TCM) system is constantly being applied and validated for curing human diseases and maintaining health over 5000 years of Chinese history and civilization, Chinese materia medica and TCM system have achieved great success and accumulated invaluable experiences in the clinic. Therapeutical effects of TCMs around the world for curing a certain intractable disease are usually from whole outcomes of multiple constituents in the clinic, no matter what single TCM or compound prescription is applied by the patients. Unfortunately, according to new drug criterion of the modern western country, some single compounds extracted and separated from a TCM sometimes can’t show definite pharmacological effects in vivo and in vitro while their extracts of original TCMs have obvious therapeutical outcomes either in lab or in the clinic. To finish a suit of GLP, GCP including Phase I–IV, drug register in the local drug administration department and GMP for developing a new drug (natural medicines also need to add GAP in addition to the above) will invest several dozen millions of research cost, undergo dozens of year. Complex drug action mechanisms and drug substances of Chinese materia medica have been worldwide problems until today, unclear effective compounds of Chinese materia medica is a great gulf in the way of TCMs modernization.
LC-ESI Q-TOF MSE is a very efficient method under high resolution of Time-of-Fly (TOF) MS and MSE module, helping accurately record, qualify and quantify parent ions, daughter ions. It is a very efficient way to help rapidly elucidate complex drug substances such as TCMs, and then further explain the pharmacological mechanisms [1,2,3]. Of course, professional natural medicine databases are essential, Reaxys database (https://www.reaxys.com/) as a new commercial product of Elsevier company is very useful to chemical scholars, as a combination of Beilstein database, Patent database and Gmelin database, it covers more than 20 million compounds and 30 million chemical reactions, developes many unique searching items such as nature products, reaction rather than SciFinder. It is currently the most comprehensive and largest database of nature products. SIOC chemical database (http://www.organchem.csdb.cn/), CAS developed by Shanghai Institute of Organic Chemistry (SIOC) is the general informational system for chemical and chemical engineering research and development. It consists of more than 20 different chemical databases, such as compound structure database, Chinese traditional medicine, chemical technical information database .
Toddalia asiatica root bark (T. asiatica), or Feilong Zhangxue in Chinese name as Miao minority medicines is dried root bark of Toddalia asiatica (L.) Lam. from Toddalia genus, Rutaceae family. Its original plant distributes in mountains, valley areas of Guizhou, Guangxi, Yunnan province in China. T. asiatica obtains dispelling pathogenic wind and pain, eliminating stasis and hemostasis, subduing swelling and detoxicating in traditional Chinese medicine system. This Miao herbal medicines is always applied in the clinic for treatment of rheumatic arthralgia, stomach pain, hemorrhage, gingival bleeding, etc., which has been recorded in ‘Quality standards of Chinese medicinal materials and ethnomedicines in Guizhou (2013 edition)’, standard number: DB52/YC059-2003 . Until now, almost reported literature were focused on whole root, stem parts of T. asiatica , but ignoring the root bark part which is main medicinal part based on traditional ethnomedicine . So it’s necessary to scientifically explore the chemical constituents in T. asiatica root bark. Therefore, we will apply such high-resolution tandem MS, assisted with natural product databases to systematically explain the hemostatic chemical constituents from T. asiatica root bark, the finding in this study will help us to deeply realize therapeutical effect of T. asiatica and promote its modernization and new drug development.
Materials and reagents
All T. asiatica were collected from Huaxi district, Guiyang city in Sept. 2013 and were identified by Professor Deyuan Chen from School of Pharmacy at Guiyang College of traditional Chinese medicine. A voucher specimen was stored at Standard Library of traditional Chinese medicine and ethnic medicine, School of Pharmacy, Guizhou Medical University. Some dirty root bark were rinsed with water to remove soil particles and were then sundried. T. asiatica samples were ground into powder of the homogenous 24 mesh before the experiment. Acetonitrile (HPLC-pure, Tedia, USA), formic acid (HPLC-pure, Aladdin, China), Methanol (HPLC-pure, Sinopharm, China), Ultra-pure water purified with a Milli-Q water purification system (USA). All other chemicals were of analytical grade.
Extraction and isolation
Ten killogram powder of the dried T. asiatica root bark was extracted nine times through maceration method with 95% ethanol in room temperature. All extraction solutions were combined, filtered by Buchner funnel and concentrated in vacuo to yield extract, which was suspended in pure water. Sequential liquid–liquid extraction for successive sample partition was performed by petroleum ether (PE, bp 60–90 °C), ethyl acetate (EA) and n-butanol (n-B). The extraction and fractionation of T. asiatica root bark were according to Fig. 1. For hemostatic activity of all different polarity fractions of T. asiatica root bark, bleeding time (BT), amount of bleeding (BA) and clotting time (CT) were as efficacy evaluation indexes by typical mouse tail-cutting method and glass capillary tube method in our previous study , average values of BT, BA and CT were (59.67 ± 12. 31) s, (4.42 ± 1.67) mg and (79.67 ± 5.57) s under administration of 1.50 g kg−1 ethyl acetate fraction of 95% ethanol extract in Kunming mice, ethyl acetate fraction was found to be the most potential part for further study on hemostatic active substance basis. Systematic separation and purification for main hemostatic natural products from T. asiatica root bark were carried out (Fig. 1).
Chromatographic separation was performed on a Waters ACQUITY UPLC instrument system coupled to a photodiode array detector (PDA), a binary pump, an autosampler and a column compartment (USA), using an Agilent ZORBAX SB-C18 RRHD column (150 mm × 2.1 mm, 1.8 μm). A two-component mobile phase consisting of acetonitrile (A): 0.1% formic acid (B) was used following the elution program: 0 min, A:B (5:95, v/v); 30 min, A:B (90:10, v/v); 40 min, A:B (100:0, v/v). The flow rate was 0.4 mL/min, column temperature was maintained at 30 °C, injection volume was 5 μL by automatic sampling system at 20 °C. An accurately-weighted ethyl-acetate extract was dissolved in absolute methanol (Met), the supernatant was filtered through a 0.22-μm lipophilic microporous filter.
ESI Q-TOF MSE conditions and data analysis
A Waters Xevo G2-XS Q-TOF (quadrupole time-of-flight) Mass Spectrometer (USA) hyphenated with the Waters ACQUITY UPLC equipped with an ESI source was applied for rapidly identifying the major constituents in ethyl acetate fraction showing hemostatic activity, based on systematic database retrieval. Mass spectrometer and UPLC system were controlled by MassLynx® v4.1 software (Waters, USA). The UPLC effluent after chromatographic separation was introduced into the ESI source without splitting ratio. Data collection was achieved by MSE methodology using two interleaved scan functions with independent collision energies. In this way, a low collision energy scan (Function 1) is immediately followed by a scan in which the collision energy (Function 2) is ramped over a higher range to induce fragmentation of the ions transmitted through the quadruple. This approach enables the simultaneous acquisition of intact precursor ions (protonated molecule [M+H]+) and related fragmentions from a single analysis. Each sample was determined in both negative and positive ion modes separately to offer sufficient fragment information.
In this experiment, the related MS parameters were programmed as follows. In MSE centroid section: (1) acquisition: acquisition times, from start time 0 min to end time 40 min; source: ES; acquisition mode, positive or negative polarity, that means each sample was analyzed in positive ion mode and negative ion mode through different sample injections; analyser mode, resolution; dynamic range, extended. (2) TOF MS: Da range, acquire low or high energy over the range of low mass 50 Da to high mass 1000 Da; scan time, 0.5 s; data format, centroid; (3) collision energy: function 1-low collision energy, off; function 2-high collision energy, 40–80 V; (4) cone voltage: override cone voltage value specified in tune file; cone voltage, 40 V. In ES−/ES+ section: source capillary, 2.8 kV; sampling cone, 30; source offset, 60; source temperatures, 120 °C; desolvation temperatures, 450 °C; cone gas flow, 50 L/h; desolvation gas flow, 800 L/h; high-purity helium (He) as collision gas, high-purity nitrogen (N2) as nebulizer and auxiliary gas. In LockSpray properties section: acquire LockSpray-apply correction; LockSpray reference compound, 554.2615 Da, leucine-enkephalin (LE); LockSpray acquisition setting: scan time, 0.1 s; interval, 10 s; scans to average, 3; mass windows; 0.5 Da.
Results and discussion
Extraction and isolation
After removing the solvent under reduced pressure, 1390.5 g ethyl acetate fraction was subjected to silica gel column chromatography (CC) and was eluted to get fractions 1–11 using a step-gradient solvent system of ethyl acetate (EA):petroleum ether (PE) (0:100 → 100:0). Fr.5 (66.0 g) was further isolated by silica gel CC with EA:PE (1:20 → 1:5) to yield Cp.23 (3.80 g) in EA:PE (1:6), Cp.24 (8.17 g) in EA:PE (1:20) and Cp.34 (676.4 mg) by recrystallization. Fr.7 (5.1 g) was further purified by LH-20 with Met, Cp.30 (21.3 mg) was finally obtained (Fig. 1). Raw spectral analysis data of these four main compounds were listed below.
Isopimpinellin (Cp.23): C13H10O5, yellow crystals. ESI–MS m/z 247.0597 [M+H]+, 269.0421 [M+Na]+. 13C NMR (101 MHz, Chloroform-d): δ 160.62 (C-2), 150.06 (C-7), 145.20 (C-2′), 144.37 (C-5), 143.68 (C-9), 139.58 (C-4), 128.10 (C-8), 114.72 (C-6), 112.75 (C-3), 107.53 (C-10), 105.25 (C-3′), 61.78 (C-8-OCH3), 60.84 (C-5-OCH3). 1H NMR (400 MHz, Chloroform-d): δ 8.06 (d, J = 6.7 Hz, 1H, H-4), 7.58 (d, J = 1.4 Hz, 1H, H-2′), 6.96 (d, J = 1.4 Hz, 1H, H-3′), 6.22 (d, J = 6.7 Hz, 1H, H-3), 4.14 (s, 3H, 8-OCH3), 4.10 (s, 3H, 5-OCH3). Compared to Ref. , isopimpinellin was confirmed.
Pimpinellin (Cp.24): C13H10O5, yellowish needles. ESI–MS m/z 247.0612 [M+H]+. 13C NMR (101 MHz, Chloroform-d): δ 161.21 (C-2), 150.15 (C-7), 145.73 (C-2′), 144.79 (C-9), 143.54 (C-5), 140.27 (C-4), 135.48 (C-6), 114.47 (C-3), 114.09 (C-8), 109.79 (C-10), 104.67 (C-3′), 62.74 (5-OCH3), 61.59 (6-OCH3). 1H NMR (400 MHz, Chloroform-d): δ 8.09 (d, J = 9.8 Hz, 1H, H-4), 7.66 (d, J = 2.2 Hz, 1H, H-2′), 7.09 (d, J = 2.2 Hz, 1H, H-3′), 6.38 (d, J = 9.8 Hz, 1H, H-3), 4.15 (3H, s, 6-OCH3), 4.04 (3H, s, 5-OCH3). Compared to Ref. , pimpinellin was finally confirmed.
Coumurrayin (Cp.30): C16H18O4, white crystals. ESI–MS m/z 275.1278 [M+H]+, 297.1095 [M+Na]+. 13C NMR (101 MHz, Chloroform-d): δ 161.88 (C-2), 160.90 (C-7), 155.26 (C-5), 153.55 (C-9), 138.93 (C-4), 132.25 (C-3′), 121.84 (C-2′), 110.77 (C-8), 110.16 (C-3), 103.80 (C-10), 90.35 (C-6), 56.06 (C-5-OCH3), 55.96 (C-7-OCH3), 25.90 (C-5′), 21.46 (C-1′), 17.99 (C-4′). 1H NMR (400 MHz, Chloroform-d): δ 7.96 (d, J = 9.6 Hz, 1H, H-4), 6.29 (s, 1H, H-6), 6.12 (d, J = 9.6 Hz, 1H, H-3), 5.18 (d, J = 7.3 Hz, 1H, H-2′), 3.91 (s, 1H, 5-OCH3), 3.90 (s, 1H, 7-OCH3), 3.41 (d, J = 7.3 Hz, 2H, H-1′), 1.80 (s, 3H, H-4′), 1.64 (s, 3H, H-5′). All data are consistent with Ref. , coumurrayin was the identified natural compound.
Phellopterin (Cp.34): C17H16O5, yellowish powder, ESI–MS m/z 301.1422 [M+H]+. 13C NMR (101 MHz, Chloroform-d): δ 160.59 (C-2), 150.78 (C-7), 145.08 (C-2′), 144.36 (C-5), 144.30 (C-9), 139.72 (C-3″), 139.46 (C-4), 126.79 (C-8), 119.80 (C-2″), 114.45 (C-6), 112.71 (C-3), 107.47 (C-10), 105.10 (C-3′), 70.36 (C-1″), 60.74 (C-5-OCH3), 25.84 (C-3″-CH3), 18.08 (C-3″-CH3). 1H NMR (400 MHz, Chloroform-d): δ 8.06 (d, J = 9.7 Hz, 1H, H-4), 7.57 (s, 1H, H-2′), 6.95 (s, 1H, H-3′), 6.22 (d, J = 9.7 Hz, 1H, H-3), 5.55 (d, J = 7.2 Hz, 1H, H-2″), 4.79 (d, J = 7.2 Hz, 2H, H-1″), 4.12 (s, 3H, 5-OCH3), 1.68 (s, 3H, 3″–CH3), 1.65 (s, 3H, 3″–CH3). These are similar to the data of Ref. , and phellopterin was confirmed.
Identification of the constituents in ethyl acetate part by ESI Q-TOF MSE
High-resolution LC–MS/MS analysis was performed to analyze the major constituents in ethyl acetate fraction showing hemostatic activity, all exact molecular weights, all negative and positive ion mode TICs and the secondary daughter ion fragments have been collected by UPLC-ESI Q-TOF MSE detection module (Fig. 2), we totally extracted 47 chromatographic peaks in UPLC-ESI-Q-TOF MSE TIC chromatograms of the liposoluble extract, the deduced molecular formulas from detected exact molecular weights were then easily calculated, commonly accepted <5 ppm threshold and >90 of Fit Conf % of the molecular formulas were finally adopted in this study (Figs. 3, 4, 5, 6). By searching reported known natural products from this herbal medicine in Reaxys database and SIOC chemical database through those deduced molecular formulas and detected exact molecular weights (Table 1), tentative identification of the constituents in ethyl acetate part of T. asiatica was done firstly. Then the conclusive research reports in the literature about this herbal medicine in CNKI  were systematically found out by searching target molecular formulas and this herbal medicine name T. asiatica, 飞龙掌血 or Feilong Zhangxue. The preliminarily deduced compounds were further screened and confirmed. After that, feasible fragmentation pathways of the very-likely candidate compounds from T. asiatica and their chemical structures were all analyzed and withstood the scientific scrutiny according to the tested negative or positive mode MSE information, the usual fragmentation pathways contained –CH3, –OCH3, –OH, –CO, –C2H4, etc. The differences of chromatographic retention behavior of similar natural compounds were also applied to ensure the correct results between those easily confused compounds.
Here we took Cp.23, Cp.29 as examples to clarify the identification process of proposed practical strategies in this study. Cp.23 was separated as a peak in tR 13.516 min its positive ion mode TICs and the secondary daughter ion fragments have been collected by UPLC-ESI Q-TOF MSE detection module, but only in positive ion mode TIC showed obvious MS signals in unit of e6. M+H 247.0597, M+Na 269.0421 and M+K 285.0159 were easily found as quasi-molecular ion peak, adduct ion peak in 1 TOF MS ES+. C13H10O5 could be calculated out by ‘Elemental Composition’ analytical tool, with mass error −3.6 ppm, −0.9 mDa and calc. mass 247.0606 [M+H]+. In Reaxys and SIOC chemical database, there were only two compounds obtaining the same molecular formula C13H10O5. Then in relation to 2 TOF MS ES+ of Cp.23, several fragment ion peaks could help me to presumably infer the possible lost fragment ions and chemical structures, Figs. 3 and 4 showed the proposed main fragmentation of Cp.23. The deprotonated precursor ion (m/z 247.0597) could be fragmented into m/z 231.0286 ([M+H–CH3]+), 217.0128 ([M+H–CH3O]+). m/z 231.0286 was forced to lose [–C2H2O] under electron bombardment or collision, and produced m/z 189.0183, then m/z 161.0239 without [–CO], m/z 133.0287 drawing off [–C2H4]. Relatively small fragment ions also could be obtained, such as m/z 105.0338, m/z 77.0022. m/z 217.0128 was not only split up into above-referenced fragment ions through losing [–CO], [–C3H4O], et al., but also divided to m/z 175.0392, m/z 143.0131, m/z 95.0130 by dissociating [–C2H2O], [–CH3O] and [–C4H6]. After the relevant research literature and reference substance comparison, Cp.23 was inferred as isopimpinellin.
The peak of Cp.29 in tR 18.186 min had a molecular formula of C16H16O4 (m/z 273.1120 for [C16H17O4]+, mass error −2.6 ppm). The deprotonated precursor ion (m/z 273.1120) was fragmented into m/z 247.0597 ([M+H–CO]+), 241.0855 ([M+H−CH3O]+) and 205.0485 ([M+H–C5H8]+). Other fragments m/z 175.0391, 161.0594, 131.0493, 115.0542, 103.0540 could be easily deduced through MS conventional cleavage, such as –CO, –OH, –CH3O (Figs. 5, 6). Reaxys database showed three natural compounds with the same molecular formula (C16H16O4) from T. asiatica (Table 1), dehydrocoumurrayin was finally confirmed through the literature reference, the polarity difference between the compounds.
Other natural products were identified following the same manner. Isopimpinellin (Cp.23), pimpinellin (Cp.24), coumurrayin (Cp.30), phellopterin (Cp.34) as main isolated compounds in this part of T. asiatica root bark through our natural product extraction and separation were also detected and verified through these strategies. As a result, a total of 31 natural compounds in T. asiatica root bark got identified or putatively characterized based on above-mentioned strategies (Figs. 7).
In this report, hemostatic chemical constituents from natural medicine T. asiatica root bark were first investigated by LC-ESI Q-TOF MSE and bioassay-guided compounds’ extraction and isolation from this natural medicine. There were totally 31 natural compounds in T. asiatica root bark which got putatively characterized, four main coumarins were purified and identified. These findings would show us more clear material basis of this complex medicine, guiding further pharmaceutical research of T. asiatica efficiently and comprehensively.
Our study illustrated that the sensitive UPLC–Q-TOF analytical system combined with the MSE method of fragmentation data collection and natural product databases, allows a relatively rapid and reasonable investigation of the reported and unknown compounds in this complex TCM sample T. asiatica. This proposed method could be a candidate strategy to study TCM or other complex natural medicines so far in facing current bottleneck situation of natural medicine development, this would better promote development and application of natural medicines and their medicinal resources.
Chinese Academy of Sciences
China national knowledge infrastructure
traditional Chinese medicine
Wang X, Zhang A, Yan G, Han Y, Sun H (2014) UHPLC-MS for the analytical characterization of traditional Chinese medicines. TrAC Trends Anal Chem 63:180–187
Fu W, Magnúsdóttir M, Brynjólfson S, Palsson BØ, Paglia G (2012) UPLC-UV-MSE analysis for quantification and identification of major carotenoid and chlorophyll species in algae. Anal Bioanal Chem 404(10):3145–3154
Plumb RS, Johnson KA, Rainville P, Smith BW, Wilson ID, Castro-Perez JM, Nicholson JK (2006) UPLC/MSE: a new approach for generating molecular fragment information for biomarker structure elucidation. Rapid Commun Mass Spectrom 20(13):1989–1994
Chinese Academy of Science. Chemistry database [DB/OL] (1978–2017). http://www.organchem.csdb.cn. Shanghai Institute of Organic Chemistry
Food and Drug administration of Guizhou Province (2003) Quality Standards of Chinese medicinal materials and ethnomedicines of Guizhou province. Guizhou Science and Technology Press, Guiyang, pp 63
Fang S, Fang G, Liu R, Yao X, Fan G (2016) Chemical constituents in Toddalia asiatica Lam.: research advances. J Int Pharm Res 43(2):239–248
Liang Jinliang, Lin Ye, Liu Liya, Zhou Wei, He Maoqiu, Hao Xiaoyan (2015) Rapid determination of four categories of pesticide residues in Toddaliae asiaticae (L.) Lam. by Carb/NH2-SPE-GC-MS. Chin Pharm J 50(5):435–441
Zhao M, Zhang X, Liu S, He M, Liang Y, Hao X, Zhou W (2016) Pharmacognostic identification and hemostatic activity of Toddalia asiatica root bark. Chin J Exp Tradit Med Formulae 22(24):32–36
Sun X, Zhang C, Li J, Feng J, Zhou H, Sheng F, Chen W (2013) Chemical constituents from Peucedanum decursivum. Chin Tradit Herbal Drugs 44(15):2044–2047
Liu S, Zhang W, He G, Lei P, Li X, Liang Y (2009) Studies on chemical constituents in stems of Zanthoxylum dissitum. China J Chin Mat Med 34(5):571–573
Yan QS, Zhang S, Wang YJ, Xiao WL, Rao GX (2008) Studies on chemical constituents of Peucedanum delavayi. J Chin Med Mater 31(8):1157–1159
Deng GG, Yang XW, Zhang YB, Xu W, Wei W, Chen TL (2015) Chemical constituents from lipophilic parts in roots of Angelica dahurica var. formosana cv. Chuanbaizhi. China J Chin Materia Med 40(11):2148–2156
China National Knowledge Infrastructure (CNKI) [DB/OL] (1999–2017). Tsinghua Tongfang Knowledge Network Technology Co., Ltd. http://www.cnki.net/. Accessed 10 Feb 2017
Phatchana R, Yenjai C (2014) Cytotoxic coumarins from Toddalia asiatica. Planta Med 80(8–9):719–722
Tsai IL, Wun MF, Teng CM, Ishikawa T, Chen IS (1998) Anti-platelet aggregation constituents from formosan Toddalia asiatica. Phytochemistry 48(8):1377–1382
Shi L, Ji Z, Yu QY, Li Y (2014) Chemical constituents in methanol parts of Toddalia Asiatica (Linn) Lam. China Pharm 17(4):534–537
Jain SC, Pandey MK, Upadhyay RK, Kumar R, Hundal G, Hundal MS (2006) Alkaloids from Toddalia aculeata. Phytochemistry 67(10):1005–1010
Lin TT, Huang YY, Tang GH, Cheng ZB, Liu X, Luo HB, Yin S (2014) Prenylated coumarins: natural phosphodiesterase-4 inhibitors from Toddalia asiatica. J Nat Prod 77(4):955–962
Tsai IL, Fang SC, Ishikawa T, Chang CT, Chen IS (1997) N-cyclohexyl amides and a dimeric coumarin from formosan Toddalia asiatica. Phytochemistry 44(7):1383–1386
Liang Y, Huang J, Zhao C, Hao X (2003) Determination of pimpinellin in Toddalia asiatica with HPLC. J Guiyang Med Coll 28(6):519–520
Hao XY, Cao XH, Liang Y, Shen YM (2004) Quantification of isopimpinellin in root of Toddalia asiatica by HPLC. China J Chin Materia Med 29(8):768–769
XYZ, WZ and LT conceived the research idea. XYZ, WBS and YL conducted the experiments, analyzed and interpreted the data as well as prepared the first draft. ZY and WZ carried out mass spectrometry analysis. XYZ, WBS, WZ and LT critically read and revised the paper. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
This work was financially supported by National Natural Science Foundation of China (81360681), “Western Light” Visiting Scholar Fund of Organization Department of the CPC Central Committee, China (2014–2015) and Guizhou Provincial Engineering and Technology Research Center for Research and Development of Chemical Synthetic Drugs (QKH2016-5402).
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Zhang, X., Sun, W., Yang, Z. et al. Hemostatic chemical constituents from natural medicine Toddalia asiatica root bark by LC-ESI Q-TOF MSE . Chemistry Central Journal 11, 55 (2017) doi:10.1186/s13065-017-0283-3
- Toddalia asiatica root bark
- Qualitative analysis
- Chemical database