- Research article
- Open Access
A validated stability-indicating HPLC method for determination of varenicline in its bulk and tablets
Chemistry Central Journalvolume 5, Article number: 30 (2011)
A simple, sensitive and accurate stability-indicating HPLC method has been developed and validated for determination of varenicline (VRC) in its bulk form and pharmaceutical tablets. Chromatographic separation was achieved on a Zorbax Eclipse XDB-C8 column (150 mm × 4.6 mm i.d., particle size 5 μm, maintained at ambient temperature) by a mobile phase consisted of acetonitrile and 50 mM potassium dihydrogen phosphate buffer (10:90, v/v) with apparent pH of 3.5 ± 0.1 and a flow rate of 1.0 ml/min. The detection wavelength was set at 235 nm. VRC was subjected to different accelerated stress conditions. The degradation products, when any, were well resolved from the pure drug with significantly different retention time values. The method was linear (r = 0.9998) at a concentration range of 2 - 14 μg/ml. The limit of detection and limit of quantitation were 0.38 and 1.11 μg/ml, respectively. The intra- and inter-assay precisions were satisfactory; the relative standard deviations did not exceed 2%. The accuracy of the method was proved; the mean recovery of VRC was 100.10 ± 1.08%. The proposed method has high throughput as the analysis involved short run-time (~ 6 min). The method met the ICH/FDA regulatory requirements. The proposed method was successfully applied for the determination of VRC in bulk and tablets with acceptable accuracy and precisions; the label claim percentages were 99.65 ± 0.32%. The results demonstrated that the method would have a great value when applied in quality control and stability studies for VRC.
Varenicline (VRC, Figure 1); 7,8,9,10-tetrahydro-6,10-methano-6H-azepino[4,5-g]quinoxaline (2R,3R), is a novel agent that is a centrally acting as a highly selective partial agonist for the nicotinic acetylcholine receptor . VRC has mixed agonistic-antagonistic properties, thus it has the therapeutic benefit of relieving the symptoms of nicotine withdrawal and cigarette craving during abstinence while blocking the reinforcing effect of nicotine in those who lapse [2–4]. VRC tartrate (Champix® and Chantix®; Pfizer) has been approved by the USA-FDA as an aid to smoking cessation . The approved regime of VRC is 1 mg for 12 weeks, starting with a 1-week titration period .
The quality of pharmaceutical product of VRC, in terms of purity and stability of the active substance and/or finished product is vital for the effective and safe delivery of its therapeutic values to the smokers. This is because the presence of impurities and/or potential degradation products may cause changing of chemical, pharmacological, and/or toxicological properties of the active drug entity [7–9]. In general, pharmaceuticals are sensitive to environmental factors such as temperature, humidity, and light. These factors usually vary during manufacturing, transportation, storage, and distribution of the finished product. For these reasons, stability testing of the active substance and the finished product is necessary for providing information about potential degradation products, possible degradation pathways of the drug, compatibility of the drug with the excipients in the finished product, and the long-term effects of the environmental factors on the active drug and its finished products. Results of stability testing are important in developing proper manufacturing process, selecting proper packaging, storage conditions, product's shelf life, and determining the expiration date [10–12]. Furthermore, the climate is widely varying worldwide, and consequently, the quality of pharmaceutical product of VRC is greatly affected by the change in the environmental factors. As well, VRC-containing tablets are imported from outside of many countries, consequently there is a major concern about its quality after exposure to transportation, storage, and other conditions. Therefore, quality control for VRC-containing tablets is critically concerned in these countries. In literature, only one report has been found describing the separation and quantitation of process-related substances to VRC tartarate , however there was no report on the stability-indicating assay for VRC. For these reasons, a stability-indicating method for determination of VRC in its bulk drug and pharmaceutical tablets is essential, particularly, such methods have become an important aspect of any analytical method validation and a part of ICH guidance for pharmaceutical industry .
The present study describes, for the first time, the development and validation of a stability-indicating HPLC method for stability evaluation and quantitative determination of VRC in the presence of its degradation products.
Results and Discussion
The initial method development was conducted on pure drug using working standards solution protected from light. The light absorption characteristics of VRC are not available in literature, therefore its ultraviolet (UV)-absorption spectrum was recorded for selecting the proper maximum absorption peak (λmax). As shown in Figure 1, the λmax of VRC is located at 235 nm. Therefore, the detection wavelength of the detector was set at this wavelength. Actual chromatographic conditions were established after number of preliminary experiments for selecting the proper mobile system. Different mobile phase systems were tested, and the selection of the proper system depended on its ability to give good separation between the pure VRC and its possible impurities and/or degradation products. Acceptable separation was achieved on Zorbax Eclipse XDB-C8 column (150 mm × 4.6 mm i.d., particle size 5 μm) using a mobile phase composed by acetonitrile:0.05 M potassium monobasic phosphate buffer of pH 3.5 (10:90, v/v) pumped with a flow rate of 1 m/min. The column temperature was kept constant at 25 ± 2°C. Under these chromatographic conditions, the run time of the sample was 6 min, and the retention time of VRC was 3.03 ± 0.06 min (n = 3) (Figure 2A).
Linearity and Sensitivity
Using the above-mentioned optimum chromatographic conditions, three independent calibration curves were constructed correlating the detector signals with the corresponding VRC concentrations. Each curve was generated by 7-concentration points; each concentration was injected in triplicates). Regression analysis for the results was carried out using the least-square method. The standard deviation values of each concentration point (triplicates) did not exceed 2%. The results revealed a good linear calibration fit in the range of 2-14 μg/ml. The calibration equation was: AU = - 4443 + 44599 C (r = 0.9998), where AU is the detector signal, C is the concentration of VRC, and r is the correlation coefficient. The high r value was indicative for the good linearity, and the low values of standard deviations of the intercept and the slope were indicative for the significant validity of the calibration points used for constructing the calibration curve.
The limit of detection (LOD) and limit of quantitation (LOQ) were calculated based on the signal-to-noise ratio . The intercept was then equal to SD0 (the estimated SD at a concentration of zero). LOD was then defined as 3SD0, and LOQ was defined as 10SD0. The LOD and LOQ values were 0.11 and 0.38 μg/ml, respectively.
Precision was measured in accordance with ICH recommendation. Five consecutive injections of VRC solution at three varying concentrations (6, 8, and 10 μg/ml) showed excellent repeatability (intra-day precision) as the relative standard deviation (RSD) of the signal values did not exceed 0.24% (Table 1). Inter-day precision (reproducibility) was determined by measuring inter-day variation for triplicate determination of VRC at the same VRC concentrations (6, 8, and 10 μg/ml). The low RSD values (≤ 0.34%) indicated the acceptable reproducibility of the method .
Accuracy was determined by the recovery study of known amounts (6 - 12 μg/ml) of VRC standard added to a placebo matrix for tablets. The samples were analyzed (5 replicates were injected) by one analyst, and the added amounts were calculated from a calibration curve. The recovery was presented as percentages, (calculated concentration/nominal concentration × 100). The recovery values ranged from 98.63 to 101.19 ( ± 0.22 - 0.55%), Table 2. These results indicated the acceptable accuracy of the method .
Robustness and Ruggedness
In order to measure the extent of the method robustness, the most critical parameters were interchanged while keeping the other parameters unchanged, and in parallel the chromatographic profile was observed and recorded. The chromatographic parameters were interchanged within the range of 1-10% of the optimum recommended conditions. The studied parameters were: the composition of the mobile phase, pH, flow rate, and column temperature. The results indicated that the small change in the conditions did not significantly affect the determination of VRC.
Ruggedness of the method was determined by using mobile phase components from two different manufactures. There was no significant change in the retention time of VRC was observed; RSD was 0.24 - 0.37% indicating the ruggedness of the method.
Sample Solution Stability
The stability of the drug in solution during analysis was determined by repeated analysis of samples during the course of experimentation on the same day and also after storage of the drug solution (8 μg/ml) for 24, 48 and 72 hours under laboratory bench condition (25 ± 1°C) and under refrigeration (8 ± 0.5°C). There was no significant change in analysis over a period of 72 hours. The mean RSD between peak areas for the samples stored under refrigeration (8 ± 1°C) and at laboratory temperature (25 ± 1°C) was found to be 0.47% and 0.24%, respectively, suggesting that the drug solution can be stored without any degradation over the time interval studied.
System Suitability Tests
The chromatographic systems used for analysis must pass the system suitability limits before sample analysis can commence. The capacity factor (K), injection repeatability (as described earlier in the subsection, precision), tailing factor (T), theoretical plate number (N) and resolution (Rs) for the principle peak and its degradation product were evaluated using VRC solution of 8 μg/ml. The capacity factor (K) was found to be 1.99 indicating that the VRC peak is well resolved with respect to the void volume. The RSD of five consecutive injections was found to be 0.24%, indicating good injection repeatability. The tailing factor (T) for VRC peak was found to be 1.25, reflecting good peak symmetry. The resolution (Rs) for the principle peak and degradation product was found to be 1.87, indicating good separation of the drug from degradation product. The theoretical plate number (N) was found to be 1179 for the column used in the study (150 mm × 4.6 mm i.d., particle size 5 μm), thus demonstrating acceptable column efficiency. All these results assure the adequacy of the proposed HPLC method for routine analysis of VRC.
The ICH guideline entitled stability testing of drug substances and products  requires the stress testing to be carried out to elucidate the inherent stability characteristics of the active substance, and provide a rapid identification of differences that might result from changes in the manufacturing processes or source sample. Susceptibility to oxidation, hydrolytic, and photolytic stability are the required tests. An ideal stability-indicating method is one that quantifies the standard drug alone and also resolves its degradation products. As described in the experimental section, different stress conditions were applied: boiling, acid, base hydrolysis, oxidation, and irradiation with UV light. From this investigation, it was clear that in case of boiling, UV irradiation (solid state and solution), and oxidation, VRC was stable under the employed stress conditions as no degradation products were observed in their chromatograms, which were identical to the chromatogram of VRC solution that has not been subjected to any stress conditions (Figure 3A). In case of acid hydrolysis and alkaline hydrolysis degradation products were observed at retention times of 1.46 and 1.37 min, respectively (Figure 3B-D). In both cases, the method was able to separate completely the degradation products from the intact VRC. This confirmed the selectivity and stability-indicating property of the proposed method. The concentration of the produced degradation products relative to the intact VRC was calculated and found to be 0.29 and 0.31% in case alkaline hydrolysis and acid hydrolysis, respectively.
Applicability of the Method
It is evident from the results obtained previously that the proposed method gave satisfactory results with the analysis of VRC in bulk. Thus, VRC-containing tablets were subjected to the analysis by the proposed method. The label claim percentage was 99.7 ± 0.32%. This acceptable value indicated the applicability of the method for the routine quality control of VRC tablets without interference from the excipients. This was evidenced from the good label claim percentage as well as the absence of any peaks in the chromatogram of the tablet extract solution (Figure 2).
Varenicline Tartarate reference standard with claimed purity of 99.6% was purchased from Weihua Pharma Co. Ltd. (Zhejiang, China). Champix® tablets (Pfizer Inc, New York, USA), labeled to contain 1 mg (as the anhydrous base) per tablet was obtained from the local market. HPLC-grade solvents, and reagent-grade sodium hydroxide, hydrochloric acid, hydrogen peroxide, potassium dihydrogen phosphate and orthophosphoric acid were purchased from Merck (Darmstadt, Germany). Distilled water purified by a Millipore Milli-Q® UF Plus apparatus (Millipore, Molsheim, France) was used throughout the work.
HPLC apparatus consisted of a Waters Alliance HPLC systems (Waters, USA) equipped with 2996 photodiode array (PDA) detector. The chromatographic separations were performed on a Zorbax Eclipse XDB-C8 column (150 mm × 4.6 mm i.d., particle size 5 μm) manufactured by Millipore, USA. The column temperature was kept constant at 25 ± 2°C. Separations were performed in isocratic mode using a mobile phase consisted of acetonitrile and 50 mM potassium dihydrogen phosphate buffer (10:90, v/v), and an apparent pH adjusted to 3.5 ± 0.1 with 10% phosphoric acid solution. The mobile phase was filtered by a Millipore vacuum filtration system equipped with a 0.45 μm filter, degassed by ultrasonic bath, and by bubbling helium gas for 5 min prior to its use. The flow rate of the mobile phase was 1 ml/min, and the sample injection volume was 10 μl. The PDA detector was set at 235 nm. Peak identity was confirmed by retention time comparison and comparison of the spectra obtained from the PDA detector.
Preparation of standard and sample solutions
An accurately weighed quantity (25 mg) of VRC reference standard material was transferred to a 25-ml volumetric flask, and dissolved in 25 ml water. This stock solution (1 mg/ml) was further diluted with water to produce a working standard solution of 8 μg/ml. Tablets sample was prepared as the following: 20 tablets were weighed and the average weight was calculated. Tablets were crushed to a fine powder, and a quantity of the powdered tablets, equivalent to 10 mg of VRC, was transferred to 50 ml volumetric flasks. A 25 ml of methanol was added, the contents of the flask were shaken for 10 min by a mechanical shaker, and the volume was diluted to 50 ml with methanol. This solution (0.5 mg/ml) was diluted to give a concentration 10 μg/ml. This solution was filtered through a 0.45 μm membrane filter and the filtrate was subjected to the analysis by the proposed method.
One milliliter of VRC solution (25 mg/ml) was transferred to a small rounded flask. The solution was subjected to reflux for 2 h in a boiling water bath. The solution was cooled to room temperature (25 ± 5°C), and a volume of 10 μl (1 mg sample) was injected into the HPLC system.
Acid and alkali hydrolysis
Aliquot of 1 ml of VRC solution (25 mg/ml) was transferred to a small rounded flask. The solution was mixed with 4 ml of 0.1 N hydrochloric acid, or 0.1 N sodium hydroxide. The prepared solutions were subjected to reflux for 2 h in a boiling water bath. The samples were cooled to room temperature (25 ± 5°C), neutralized with an amount of acid or base equivalent to that of the previously added. From the resulting neutral solution, 10 μl (1 mg) was injected into the HPLC system.
One milliliter of VRC solution (25 mg/ml) was transferred to rounded flask. The contents were then mixed with 4 ml of 30% hydrogen peroxide solution, and the reaction mixture was allowed to proceed at room temperature (25 ± 5°C) for 2 h with intermittent shaking. A volume of 10 μl (1 mg) was injected into the HPLC system
Irradiation with ultraviolet light
A sample powder of VRC (25 mg) was exposed to UV light (245 nm) for 72 hours. The material was dissolved in 5 ml water. The solution was filtered with syringe filtration disk and diluted to 25 ml with suitable solvent to obtain a claimed concentration of 1 mg/ml. A volume of 10 μl was injected into the HPLC system. As well, an aqueous solution of VRC (1 mg/ml) was exposed to UV light (245 nm) for 72 hours, and then 10 μl was injected into the HPLC system
The present study represents the first report that deals with the development of a stability-indicating HPLC method for determination of VRC. This study is a typical example of development of a stability-indicating assay, established following the recommendations of ICH/FDA guidelines. The proposed method showed acceptable accuracy, precision, selectivity, and wide linear concentration range. From the economical point of view, the method involved the native UV-absorbing property of VRC, rather than expensive derivatizing analytical reagents. Statistical analysis for the results proved that the method is suitable for the determination of VRC in bulk and tablet forms without any interference from the degradation products, and it is recommended for routine use in quality control industry laboratories.
high-performance liquid chromatography
International Conference on Harmonization
Food and Drug Administration
maximum absorption peak
limit of detection
limit of quantification
relative standard deviation
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The authors extend their appreciation to the Deanship of Scientific Research at King Saud University for funding the work through the research group No. RGP-VPP-065.
The authors declare that they have no competing interests.
AK participated in the design of the study and preparing the draft manuscript. MM carried out the optimization of chromatographic conditions and stability testing. MK participated in stability testing and application of the method. ID participated in the study design, statistical analysis of the data and preparing the manuscript.
About this article
- Potassium Dihydrogen Phosphate
- Phosphoric Acid Solution
- Theoretical Plate Number
- Potassium Dihydrogen Phosphate Buffer