Table 1 Immobilization of Candida rugosa lipase on MWNT. Different units of CRL were immobilized on 1 mg of MWNTs. The hydrolytic activity was determined on MWNTs, supernatant and washings. All experiments were carried out in duplicate and the results within each pair differed by < 5%.
Loaded Units | Supernatant + washings | Expressed activity (B) | Theoretical activity (A) | B/A |
|---|---|---|---|---|
0.74 | 0.04 | 0.30 | 0.70 | 0.42 |
1.48 | 0.24 | 0.65 | 1.24 | 0.52 |
2.9 | 0.29 | 1.39 | 2.60 | 0.53 |
5.8 | 1.63 | 2.04 | 4.17 | 0.48 |