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Characterization of Italian honeys (Marche Region) on the basis of their mineral content and some typical quality parameters



The characterization of three types of Marche (Italy) honeys (Acacia, Multifloral, Honeydew) was carried out on the basis of the their quality parameters (pH, sugar content, humidity) and mineral content (Na, K, Ca, Mg, Cu, Fe, and Mn). Pattern recognition methods such as principal components analysis (PCA) and linear discriminant analysis (LDA) were performed in order to classify honey samples whose botanical origins were different, and identify the most discriminant parameters. Lastly, using ANOVA and correlations for all parameters, significant differences between diverse types of honey were examined.


Most of the samples' water content showed good maturity (98%) whilst pH values were in the range 3.50 – 4.21 confirming the good quality of the honeys analysed. Potassium was quantitatively the most relevant mineral (mean = 643 ppm), accounting for 79% of the total mineral content. The Ca, Na and Mg contents account for 14, 3 and 3% of the total mineral content respectively, while other minerals (Cu, Mn, Fe) were present at very low levels. PCA explained 75% or more of the variance with the first two PC variables. The variables with higher discrimination power according to the multivariate statistical procedure were Mg and pH. On the other hand, all samples of acacia and honeydew, and more than 90% of samples of multifloral type have been correctly classified using the LDA. ANOVA shows significant differences between diverse floral origins for all variables except sugar, moisture and Fe.


In general, the analytical results obtained for the Marche honeys indicate the products' high quality. The determination of physicochemical parameters and mineral content in combination with modern statistical techniques can be a useful tool for honey classification.


The Community Directive [1] establishes the general definition of honeys that can be marketed in the European Union. The Directive also indicates general and specific compositional characteristics of honey such as sugar content, humidity, acidity, electrical conductivity, diastase activity and hydroxymethylfurfural (HMF) content. Furthermore, labels on honey packaging may be supplemented to include information on the product's regional or topographical origin, floral or vegetable origin, or even specific quality criteria.

Honey is defined as "the natural sweet substance produced by Apis mellifera bees from the nectar of plants or from secretions of living parts of plants or excretions of plant-sucking insects on the living parts of plants, which the bees collect, transform by combining with specific substances of their own, deposit, dehydrate, store and leave in honeycombs to ripen and mature" [1]. The beneficial characteristics of honey are its high nutritional value (330 kcal/100 g) and the fast absorption of its carbohydrates on consumption. Moreover, honey exhibits anti-bacterial and anti-inflammatory properties in the treatment of skin wounds and many gastrointestinal diseases [25]. This is due to honey's high osmotic pressure, acidity and the hydrogen peroxide content [3, 4]. Hydrogen peroxide produced enzymatically is responsible for honey's antibacterial activity.

Italy has the highest number of honey varieties in Europe: 32 unifloral and different varieties of multifloral honeys are produced from a total of 1.070.262 hives [6]. In 2004, honey production reached about 10.200 tons/yr. At present, Italian honeys are strongly affected by competition from Argentine and Chinese varieties whose prices are lower by roughly 50%. Very scarce data are available regarding honey production in the Marche region because of the strong amatorial characteristic of such production and there is some uncertainty in the evaluation of production levels. The commonest honeys produced in the Marche region are multifloral (Millefiori) and acacia honeys (unifloral, Robinia pseudoacacia). The 2004 production levels in the Marche region were roughly 15 and 20 kg/hive for multifloral and acacia honeys respectively, with a total of 38.000 hives and 209.000 tons of total produced honey [6].

Usually, honey is considered unifloral when the pollen frequency of one plant is over 45% [7]. For honey samples with under-represented pollen grains (i.e. Lavender, Citrus and Rosemary), botanical classification may be achieved with a percentage pollen frequency of only 10–20% [814].

Melissopalynology, identification and quantification of pollen grains contained in honey, have together been the traditional method used to ascertain the botanical origin of honeys [7, 15, 16], but this technique has some limitations [1722]. A particular difficulty is that melissopalynology requires previous knowledge of pollen morphology and specialised professional personnel to achieve reliable results [23]. However, nowadays in spite of these problems melissopalynology remains the reference method.

The composition and properties of a particular honey sample depend highly on the type of flowers visited by the bees, as well as on the climatic conditions in which the plants grow [2426]. Honeybees and their products can also be employed as potential bioindicators of environmental contamination [27]. These specific chemical and physical properties may be used for the determination of the botanical origin of honey [18, 2830] and to confirm the results of microscopical analysis.

In recent decades several studies have evaluated some chemical and physicochemical components of honeys, in addition to attempting to establish representative ranges of some of these parameters that would unequivocally determine botanical origin. In characterising unifloral honeys, many authors [11, 18, 20, 22, 25, 3137] have suggested the use of physicochemical parameters (i.e. pH, sugar content, electrical conductivity, proline, enzymatic activity, water content, ash content, diastase activity, free and lactonic acidities, etc.) and mineral content (K, Ca, Na, Mg, Fe, etc.) complemented by pollen analysis.

The goal of the present work was first, to verify some of the qualitative parameters such as pH, sugar content and humidity, and second, to contribute to the very scarce available data on mineral content of Marche Region honeys. The elements assessed were: Na, K, Ca, Mg, Fe, Cu and Mn. Furthermore, we have evaluated if the physicochemical parameters and mineral content of Marche honeys can determine the botanical origin. The sampling protocol was made up in order to obtain the most representative insight of the sampled regional areas. All samples were collected in the Montefeltro region, in the Pesaro – Urbino province, a relevant production zone for many typical food products of the Marche region.

Results and discussion

Table 1 reports the mineral content and physicochemical parameters of honey samples taken from the Marche Region. The mean, standard deviation and the variable ranges are reported according to their botanical origin.

Table 1 Descriptive Equality of means testscontent (μg g-1 dry weight) in Marche honey samples.

The pH is indeed a useful index of possible microbial contamination [38] and has high relevance during the extraction and storage of honey because it is related to the stability and the shelf life of the product [39]. As previously reported [40], most bacteria and moulds grow in a neutral and mildly alkaline environment respectively, while yeasts require an acidic environment (pH = 4.0 – 4.5) and do not grow in alkaline media. The analyzed honeys show a mean pH value of 3.70 with a range of between 3.50 – 4.21. The mean pH value of Marche honeys was lower than that reported by Conti (2000) [38] for Lazio (central Italy) honeys, by Downey et al. (2005) [41] for floral honeys collected in Ireland and by Serrano et al. (2004) [20] for Andalusia (Spain) honeys. The pH values showed a very good correlation with K levels in honeys (r = 0.766; p = 0.05).

Water content is strictly related to climatic conditions and the degree of maturity; anomalous values may be an index of adulterations. The water content generally depends on the botanical origin of the sample, the processing techniques and the storage conditions [38]. Mean humidity was 17.4 % with a range of between 15.1 – 21.0 %. Only 3 samples out of 69 showed levels of humidity slightly higher than the limit permitted by the Council Directive of 20% [1]. This confirms that the fermentation rate is very low in the analyzed samples. Reported data for humidity were very similar for the three honey types analyzed, showing very low SD levels (see table 1). Moisture values observed for Marche honeys were higher than those obtained for Lazio [38], Andalusia [20] and Greece [12] honeys, but similar results were found by Downey et al. (2005) [41] for Ireland unifloral honeys.

The average sugar content was 81.16 % and the range was 77.60 – 83.80 %. Sugar content showed normal levels similar to those reported for Spanish thyme honeys [39]. Sugar and moisture content, as previously reported for Lazio honeys, are strictly correlated [38]. This study confirms the very good correlation value (r = 0.996; p = 0.01) between these quality parameters.

Mean mineral contents were (μg g-1 dry wt.): Na, 24.5; K, 643; Ca 116; Mg, 21.2; Cu, 0.81; Fe, 6.34; Mn, 0.44. The mean fresh weight/wet weight ratio was 1.21 (n = 69). For data comparison, the reported results were appropriately transformed (i.e. wet or dry basis) when necessary.

Potassium, which accounts for 79% of the total mineral content, was quantitatively the most abundant of the elements present. This result is consistent with other reported data [42, 43].

Our mean K levels were higher than for Lazio honeys [40, 38] and mean levels in Morocco honeys [44], but smaller than those reported for Spanish honeys collected from different regions [45] and Slovenian honeys [46].

The mean sodium content (24.5 μg g-1 ) was significantly lower than in Lazio [38] and Spanish honeys [45], whose contents were 80.0 and 75.7 μg g-1 d.w. respectively.

Magnesium levels (21.24 μg g-1 ) were lower than in Lazio [38], Morocco [44] and Spanish honeys [45], that were 30.85, 32.05 and 38.98 μg g-1 respectively, but higher than those for Turkish honeys [47].

Calcium levels (116.1 μg g-1 ) were significantly higher than for Lazio honeys [38] and Slovenian honeys [46]. Moreover, the levels of Ca reported here are lower than for Spanish honeys [45] that was 168.8 μg g-1 .

The mean iron level in Marche honeys (6.34 μg g-1 ) was significantly higher than for Lazio [38] and Turkish honeys [47] and lower than for Morocco honeys [44].

The mean cooper level for the Marche honeys (0.81 μg g-1 ) is very similar to that reported by Terrab et al. (2003) [44] and Fernàndez Torres et al. (2005) [45]. The mean manganese level (0.45 μg g-1 ) was lower with respect to those found to Lazio [38], Morocco [44], Spanish [45] and Slovenian honeys [46].

Potassium showed positive correlation with Ca (r = 0.645), Mn (r = 0.670) and Mg (r = 0.759). A very high positive correlation was also found between Ca and Mg, that is, r = 0.928. Moreover, Na correlated with Ca (r = 0.825) and Mg (r = 0.826).

From the results of the Kolmogorov test, the distributions within each honey type can be considered normal (p-value < 0.05), but the Levene test of the homogeneity of variances shows that there are differences among the factor levels (honey types) for some variables. For this reason, Welch's robust test for the equality of means was conducted.

The one-way ANOVA (table 2), which considered floral origin as main factor, shows that statistically significant differences were found for all studied parameters with the exception of sugar, Fe, and moisture. Thus, these variables were not considered in the application of LDA. These results showed that moisture is not associated with the botanical origin of honeys, as also reported by other authors [20, 22, 48]. Contrarily, some studies have reported that moisture is related with botanical origin [35, 37].

Table 2 Equality of means tests.

Table 3 shows the factor loading obtained for the first two factors and the variance explained by each of them. The first two principal components accounted for more than 75% of the variation in the honey samples analysed. The first principal component (PC1) explains 59.8% of the variance, and the second (PC2) explains 17.3% of the variance. According to the loading matrix (table 3), it can be observed that Mg and K are the dominant parameters in the first factor, while Ca, Na and Mn showed slightly lower values. Similar results, for K and Mg, were reported by other authors [44, 45]. The factor loading in PC2 showed that pH resulted the most dominant variable in this PC.

Table 3 Principal component analysis (PCA). Loadings of the variables, eigenvalues, explained and cumulative variance for the first two first PCs.

Examining the graphical distribution of the honey samples on the reported plot (figure 1) using the PC1 and PC2 principal components as coordinate axes, a natural separation of the three honey groups of different botanical origin was found. However, some multifloral and acacia honey samples did overlap. PCA results suggested that physicochemical parameters and mineral component data could provide useful information to achieve a botanical classification for the investigated honey.

Figure 1
figure 1

Principal component score plot. (Botanical origins: Acacia, Multifloral, Honeydew).

Wilks's lambda test (table 4) shows that each discriminant function is significant (p-value < 0.05) thus allowing each to be used for model interpretation. Table 4 also shows the eigenvalues, the percentage variance explained by each function, the cumulative percentage variance explained and canonical correlation (R). These results shows that the first discriminant function is the most important in honey sample classification.

Table 4 Tests of significance, eigenvalues and canonical correlation for the discriminant functions.

The standardized discriminant coefficients (table 5) are used to compare the relative importance of the independent variables, for instance, beta weights are used in regression [49, 50]. The higher the absolute value of a standardized coefficient, then the more significant is the related selected variable in the canonical variable. Mg resulted in being the parameter that contributes most to the first canonical variable (standardized coefficient = 0.893), accounting for most of the discrimination between honey classes (~91%) while K and pH show lower values.

Table 5 Standardized coefficients for canonical variables obtained by discriminant analysis.

For DF1, Mg is the most important variable in explaining the separation in the honey samples according to botanical origin. The second canonical variable is related positively to pH and negatively to Mg, as deduced from the high absolute values of the standardized coefficients (1.04, and -1.0, respectively). This explains more than 9% of the variance. The loading in DF2 shows that pH is the most important variable in explaining the separation between honey samples. In fact, pH has been previously described as a possible indicator of the botanical origin for honeys [22, 37, 48].

The scatter diagram of honey samples, the axes of which are the first two canonical variables (figure 2), shows that three types of honeys appear completely separated in the plot.

Figure 2
figure 2

Canonical plots: honeys are located in the space formed by two discriminant functions (Botanical origins: Acacia, Multifloral, Honeydew, □ Group centroid).

LDA can be also used to predict the group membership of honeys. The results of classification, when all the samples were in the training set, are shown in table 6. It reports the number (and percentages) of samples correctly classified into each honey type (on the diagonal of the matrix) and those that were misclassified. The LDA total error of classification was very low (0.8%).

Table 6 Classification results of LDA of seven variables (pH, Na, K, Ca, Mg, Cu, Mn) in multifloral and unifloral Marche honeys.

All acacia, honeydew, and multifloral honey samples were correctly classified into their a priori established honey types. Generally, it is not difficult to obtain very good classification if the same cases are used for the model estimation. In order to have a more exact idea of the forecast LDA performance, it is more useful to classify cases that were not previously used for the estimation of the LDA model, such as cross-validation methods.

The "leave-one-out" method [51] was performed. This method classifies a particular sample by considering the whole set of samples but excluding the contribution of the sample being classified.

Table 6 shows the results of this study. Acacia and honeydew honey samples were correctly classified in their a priori established honey types (100%), while multifloral honeys show slightly lower agreement percentages (97.7%).

In conclusion, the analytical results obtained for the Marche honeys indicated a good level of quality of this product. The determination of physicochemical parameters and mineral content in combination with modern statistical techniques is a useful tool for honey classification. In this study PCA explained more than 75% of the variance with the first two PC variables. The variables with higher discrimination power, according to the multivariate statistical procedure, were Mg and pH. On the other hand, all samples of acacia and honeydew, and more than 90% of samples of multifloral type have been correctly classified by using the LDA.


In general, the analytical results obtained for the Marche honeys indicate the products' high quality. The determination of physicochemical parameters and mineral content in combination with modern statistical techniques can be a useful tool for honey classification. However, more studies are needed in order to characterize unifloral and multifloral honeys by means of pattern recognition methods of zones of relevant honey production.



The study was conducted on 69 samples of the typical honeys produced in the Marche Region in central Italy: 44 multifloral, 23 acacia, 2 honeydew. All collected samples were taken from the local beekeepers' association with a guarantee of genuineness. All samples were collected and stored in holders and immediately transferred to the laboratory where they were kept at 4–5°C until analysis.

pH, sugar content and moisture

The pH was assessed by means of a potentiometer utilizing a pH meter Mettler Delta 345 (Mettler Toledo, Milano, Italy) [52]. Sugar and moisture values were determined utilizing a special refractometer Bertuzzi (Bertuzzi, Milano, Italy) owing two direct reading displays, for the measurement of sugar content and moisture percent respectively (Chatway method). Sugar content was expressed as brix degrees [52].

Determination of mineral elements

About 0.6–0.7 g of fresh honey was treated with 8 ml of 70 % (w/w) Nitric Acid Suprapur (Merck, Suprapur, Darmstadt, Germany) and 2 ml of 30 % (w/w) Hydrogen Peroxide Suprapur (Merck, Darmstadt, Germany) in PTFE vessels. The microwave closed digestion system (MDS 2000, CEM Corporation, North Caroline, USA) was used for the mineralization process. The treatment procedure was programmed in five steps with a power of 600 W applied for 5 min at each; the pressure in the system was set as follows: 20, 40, 85, 140 and 200 psi. Subsequently, digestion vessels were cooled to room temperature. The final clear solution was made up to 50 mL with DWI water. Simultaneously, duplicate digestion blanks were prepared.

All mineral elements in digested solutions were determined using a Shimadzu 6800 Atomic Absorption Spectrometer (Kyoto, Japan) coupled to different atomic vapor generators depending of analytical concentration. A graphite furnace accessory GFA-6000 and autosampler ASC-6000 were employed for Cu and Mn measurements and a flame of air/acetylene was used for Fe, Ca, Mg, Na and K.

All chemicals used in sample treatments were ultra-pure grade (HNO3, H2O2 30%, Merck, Suprapur, Darmstadt, Germany). Ultra-pure water (Milli-Q system, Millipore Corporation, U.S.A.) was used for all solutions. All glassware was cleaned prior to use by soaking in 10 % v/v HNO3 for 24 hours before rinsing with Milli-Q water. The standard metal solutions were prepared from stock standard solutions of ultra-pure grade supplied by Merck (Darmstadt, Germany).

The traceability of results was obtained from the analysis of the standard reference material NIST-1515 (apple leaves – National Institute of Standards and Technology) and the certified reference material Antartic Krill MURST-ISS-A2 (Italian Research Programme in Antártica). Table 7 shows the results obtained for Na, K, Ca, Mg. Cu, Fe and Mn in both materials. A sample of reference material and blanks was included in each analytical batch. Results were in very good agreement with certified values for all the elements considered proving good repeatability of the method employed.

Table 7 Results of the analysis of standard reference materials (μg g-1 for Na, Cu, Mn, Fe and wt. percent for K, Ca, Mg, dry wt material)

Statistical methods

The mean values of water content, pH, sugar and mineral concentration of the studied honeys (Acacia, Multifloral and Honeydew) were statistically compared by one-way analysis of variance (ANOVA) and the robust Welch test. Normality and homogeneity of variances in the data were verified using Kolmogorov-Smirnov and Levene tests. Bivariate correlations (by means of Pearson's correlation coefficient) between all considered parameters were studied in order to define which were of significance. Multivariate statistical techniques such as principal component analysis (PCA) and linear discriminant analysis (LDA) were used to determine the variables that better discriminate between honey types. The SPSS software version 13.0 and R 2.2.0 were used for all the chemometric calculations.

PCA is a classic technique to reduce the dimension of the initial data representing the original data matrix X as a product of two matrices, the score matrix and the loading matrix, by projecting the raw data onto a few-dimensional space (the principal components). Principal components (PCs) are not correlated and those that are first explain the major data variability [50, 53]. The traditional approach is to use the first few PCs in data analysis since they capture most of the variation in the original data set. In this work PCA was used in order to visualize the relative distribution of the honey samples according to their botanical origin.

LDA is a widely used tool in pattern recognition. Given a nominal group variable and several quantitative attributes, the LDA extracts a set of linear combinations of the quantitative variables (called discriminant functions or canonical variables) that best reveal the differences among the groups by maximising the ratio of the sum of squares between-classes and the sum of squares within-classes [49]. The first discriminant function (DF1) extracted is that which separates the groups to a maximum. The second DF, orthogonal to the first, separates the groups based on variance not yet explained by the first DF. In this way their contributions to the discrimination between groups do not overlap. If the number of groups considered is p, there are p - 1 canonical variables that are orthogonal [49, 54, 55].


  1. Council Directive of the European Union: Council Directive 2001/110/EC of 20 December 2001 relating to honey. Official Journal of the European Communities, L10. 2002, 47-52.

    Google Scholar 

  2. Greenwood D: Honey for superficial wounds and ulcers. Lancet. 1993, 341 (8837): 90-91. 10.1016/0140-6736(93)92564-A.

    Article  CAS  Google Scholar 

  3. Taormina PJ, Niemira BA, Beuchat LR: Inhibitory activity of honey against foodborne pathogens as influenced by the presence of hydrogen peroxide and level of antioxidant power. Int J Food Microbiol. 2001, 69: 217-225. 10.1016/S0168-1605(01)00505-0.

    Article  CAS  Google Scholar 

  4. Molan P: Why honey is effective as a medicine: 2. The scientific explanation of its effects. Bee World. 2001, 82: 22-40.

    Article  Google Scholar 

  5. Cooper RA, Molan PC, Harding KG: The sensitivity to honey of Gram-positive cocci of clinical significance isolated from wounds. J Appl Microbiol. 2002, 93: 857-863. 10.1046/j.1365-2672.2002.01761.x.

    Article  CAS  Google Scholar 

  6. Osservatorio nazionale della produzione e del mercato del miele: Andamento produttivo e di mercato del miele in Italia (2005). Rapporto annuale 2004, Castel San Pietro Terme, 29.1.2005.

  7. Maurizio A: Microscopy of honey. Honey: A Comprehensive Survey. Edited by: Crane E. Heinemann, London 1979, 240-257.

  8. Serra Bonvehí J: Physicochemical properties, composition and pollen spectrum of Eucalyptus honey produced in Spain. Anales de Bromatología. 1989, 41: 41-46.

    Google Scholar 

  9. Martínez Gomez ME, Guerra Hernández E, Montilla Gomez JY, Molins Marin JL: Physicochemical analysis of Spanish commercial Eucalyptus honeys. J Apic Res. 1993, 32: 121-126.

    Google Scholar 

  10. Serra Bonvehí J, Ventura Coll F: Characterization of Citrus honey (Citrus spp.) produced in Spain. J Agric Food Chem. 1995, 43: 2053-2057. 10.1021/jf00056a018.

    Article  Google Scholar 

  11. Persano Oddo L, Piazza MG, Sabatini AG, Accorti M: Characterization of unifloral honeys. Apidologie. 1995, 26: 453-465.

    Article  Google Scholar 

  12. Thrasyvoulou A, Manikis J: Some physicochemical and microscopic characteristics of Greek unifloral honeys. Apidologie. 1995, 26: 441-452.

    Article  CAS  Google Scholar 

  13. Perez-Arquillue C, Conchello R, Arino A, Juan T, Herrero A: Physicochemical attributes and pollen spectrum of some unifloral Spanish honeys. Food Chem. 1995, 54: 167-172. 10.1016/0308-8146(95)00022-B.

    Article  CAS  Google Scholar 

  14. Seijo MC, Jato MV, Aira MJ, Iglesias I: Unifloral honeys of Galicia (north-west Spain). J Apic Res. 1997, 36: 133-139.

    Google Scholar 

  15. Louveaux J, Vergeron P: Étude du spectre pollinique de quelques miels espagnols. Ann Abeille. 1964, 1: 329-347.

    Article  Google Scholar 

  16. Louveaux J, Maurizio A, Vorwohl G: Methods of melissopalynology. Bee World. 1978, 59: 139-157.

    Article  Google Scholar 

  17. Von der Ohe W: Unifloral honeys: chemical conversion and pollen reduction. Grana. 1994, 33: 292-294.

    Article  Google Scholar 

  18. Latorre MJ, Peña R, Pita C, Botana A, García S, Herrero C: Chemometric classification of honeys according to their type. II. Metal content data. Food Chem. 1999, 66: 263-268. 10.1016/S0308-8146(98)00217-9.

    Article  CAS  Google Scholar 

  19. Hermosin I, Chicón RM, Dolores Cabezudo M: Free amino acid composition and botanical origin of honey. Food Chem. 2003, 83: 263-268. 10.1016/S0308-8146(03)00089-X.

    Article  CAS  Google Scholar 

  20. Serrano S, Villarejo M, Espejo R, Jodral M: Chemical and physical parameters of Andalusian honey: Classification of Citrus and Eucalyptus honeys by discriminant analysis. Food Chem. 2004, 87: 619-625. 10.1016/j.foodchem.2004.01.031.

    Article  CAS  Google Scholar 

  21. Devillers J, Morlot M, Pham-Delegue MH, Dore JC: Classification of monofloral honeys based on their quality control data. Food Chem. 2004, 86: 305-312. 10.1016/j.foodchem.2003.09.029.

    Article  CAS  Google Scholar 

  22. Soria AC, González M, De Lorenzo C, Martínez-Castro I, Sanz J: Characterization of artisanal honeys from Madrid (Central Spain) on the basis of their melisssopalynological, physicochemical and volatile composition data. Food Chem. 2004, 85: 121-130. 10.1016/j.foodchem.2003.06.012.

    Article  CAS  Google Scholar 

  23. Cometto PM, Faye PF, Di Paola Naranjo RD, Rubio MA, Aldo MAJ: Comparison of free amino acids profile in honey from three Argentinian regions. J Agr Food Chem. 2003, 51: 5079-5087. 10.1021/jf021081g.

    Article  CAS  Google Scholar 

  24. Abu-Tarbousch HM, Al-Kahtani HA, El-Sarrage MS: Floral-type identification and quality evaluation of some honey types. Food Chem. 1993, 46: 13-17. 10.1016/0308-8146(93)90068-Q.

    Article  Google Scholar 

  25. Salinas F, Montero De Espinosa V, Osorio E, Lozano M: Determination of mineral elements in honey from different floral origins by flow injection analysis coupled to atomic spectroscopy. Revista Española de Ciencia y Tecnología de Alimentos. 1994, 34: 441-449.

    CAS  Google Scholar 

  26. Perez-Arquillue C, Conchello P, Arino A, Juan T, Herresa A: Quality evaluation of Spanish rosemary (Rosomarinus officinalis) honey. Food Chem. 1994, 51: 207-210. 10.1016/0308-8146(94)90258-5.

    Article  CAS  Google Scholar 

  27. Conti ME, Botrè F: Honeybees and their products as potential bioindicators of heavy metals contamination. Environ Mon Ass. 2001, 69: 267-282. 10.1023/A:1010719107006.

    Article  CAS  Google Scholar 

  28. Sanz S, Pérez C, Herrera A, Sanz M, Juan T: Application approach to the classification of honey by geographic origin. J Sci Food Agric. 1995, 69: 135-140. 10.1002/jsfa.2740690202.

    Article  CAS  Google Scholar 

  29. Paramas AMG, Barez JAG, Garcia-Villanova RJ, Pala TR, Albajar RA, Sanchez JS: Geographical discrimination of honeys by using mineral composition and common chemical quality parameters. J Agr Food Chem. 2000, 80: 157-165.

    Article  CAS  Google Scholar 

  30. Bogdanov S, Ruoff K, Persano Oddo L: Physico-chemical methods for the characterisation of unifloral honeys: a review. Apidologie. 2004, 35: S4-S17. 10.1051/apido:2004047.

    Article  Google Scholar 

  31. Accorti M, Persano L, Piazza MG, Sabatini AG: Schede di caratterizzazione delle principali qualità di mieli Italiani. Apicoltura. 1989, 2: 5-35.

    Google Scholar 

  32. Krauze A, Zalewski RL: Classification of honeys by principal component analysis on the basis of chemical and physical parameters. Z Lebensm Unters Forsch. 1991, 192: 19-23. 10.1007/BF01201436.

    Article  CAS  Google Scholar 

  33. Foldházi G, Amtmann M, Fodor P, Ittzés A: The physico-chemical properties and composition of honeys of different botanical origin. Acta Alimentaria. 1996, 25: 237-256.

    Google Scholar 

  34. Lopez B, Latorre MJ, Fernandez MI, Garcia MA, Garcia S, Herrero C: Chemometric classification of honeys according to their type based on quality control data. Food Chem. 1996, 55: 281-287. 10.1016/0308-8146(95)00087-9.

    Article  CAS  Google Scholar 

  35. Mateo R, Bosch-Reig F: Classification of Spanish unifloral honeys by discriminant analysis of electrical conductivity, color, water content, sugars, and pH. J Agr Food Chem. 1998, 46: 393-400. 10.1021/jf970574w.

    Article  CAS  Google Scholar 

  36. Sánchez MD, Huidobro JF, Mato I, Muniategui S, Sancho MT: Correlation between proline content of honeys and botanical origin. Dtsch Lebensm Rundsch. 2001, 97: 171-175.

    Google Scholar 

  37. Corbella E, Cozzolino D: Classification of the floral origin of Uruguayan honeys by chemical and physical characteristics combined with chemometrics. Food Sci Technol. 2006, 39: 534-539.

    CAS  Google Scholar 

  38. Conti ME: Lazio region (central Italy) honeys: determination of mineral content and typical quality parameters. Food Contr. 2000, 11: 459-463. 10.1016/S0956-7135(00)00011-6.

    Article  CAS  Google Scholar 

  39. Terrab A, Recamales AF, Hernanz D, Heredia FJ: Characterisation of Spanish thyme honeys by their physicochemical characteristics and mineral contents. Food Chem. 2004, 88: 537-542. 10.1016/j.foodchem.2004.01.068.

    Article  CAS  Google Scholar 

  40. Conti ME, Saccares S, Cubadda F, Cavallina R, Tenoglio CA, Ciprotti L: Il miele nel lazio: indagine sul contenuto in metalli in tracce e radionuclidi. Riv Sci Alim. 1998, 2: 107-119.

    Google Scholar 

  41. Downey G, Hussey K, Daniel Kelly J, Walshe TF, Martin PG: Preliminary contribution to the characterisation of artisanal honey produced on the island of Ireland by palynological and physico-chemical data. Food Chem. 2005, 91: 347-354. 10.1016/j.foodchem.2004.06.020.

    Article  CAS  Google Scholar 

  42. White JW: Honey. Advances in food research. Edited by: Chichester CO, Mrak EM, Stewart GF. 1978, Academic Press; New York, USA, 24: 287-374.

    Google Scholar 

  43. Rodriguez-Otero JL, Paseiro P, Simal J, Cepeda A: Mineral content of the honeys produced in Galicia (North-west Spain). Food Chem. 1994, 49: 169-171. 10.1016/0308-8146(94)90154-6.

    Article  Google Scholar 

  44. Terrab A, González AG, Díez MJ, Heredia FJ: Mineral content and electrical conductivity of the honeys produced in Northwest Morocco and their contribution to the characterisation of unifloral honeys. J Sci Food Agric. 2003, 83: 637-643. 10.1002/jsfa.1341.

    Article  CAS  Google Scholar 

  45. Fernández-Torres R, Pérez-Bernal JL, Bello-López MÁ, Callejón-Mochón M, Jiménez-Sánchez JC, Guiraúm-Pérez A: Mineral content and botanical origin of Spanish honeys. Talanta. 2005, 65: 686-691. 10.1016/j.talanta.2004.07.030.

    Article  Google Scholar 

  46. Golob T, Doberšek U, Kump P, Nečemer M: Determination of trace and minor elements in Slovenian honey by total reflection X-ray fluorescence spectroscopy. Food Chem. 2005, 91: 593-600. 10.1016/j.foodchem.2004.04.043.

    Article  CAS  Google Scholar 

  47. Erbilir F, Erdoğrul Ö: Determination of heavy metals in honey in Kahramanmaraş city, Turkey. Environ Mon Ass. 2005, 109: 181-187. 10.1007/s10661-005-5848-2.

    Article  CAS  Google Scholar 

  48. Terrab A, Díez MJ, Heredia F: Characterisation of Moroccan unifloral honeys by their physicochemical characteristics. Food Chem. 2002, 79: 373-379. 10.1016/S0308-8146(02)00189-9.

    Article  CAS  Google Scholar 

  49. Huberty CJ: Applied discriminant analysis. 1994, New York :John Wiley & Sons, Inc

    Google Scholar 

  50. Johnson RA, Wichern DW: Applied Multivariate Statistical Analysis. 2002, Prentice-Hall, Upper Saddle River, NJ

    Google Scholar 

  51. Lachenbruch PA, Mickey MR: Estimation of error rates in discriminant analysis. Technometrics. 1968, 10: 1-11. 10.2307/1266219.

    Article  Google Scholar 

  52. Association of Official Analytical Chemists (AOAC): Official methods of analysis. 1990, Arlington: Association of Official Analytical Chemists, Inc, 15

    Google Scholar 

  53. Fabbris L: Statistica Multivariata. 1997, Milano: McGraw-Hill

    Google Scholar 

  54. Klecka WR: Discriminant analysis. Quantitative Applications in the Social Sciences Series, No. 19. 1980, Thousand Oaks, CA: Sage Publications

    Google Scholar 

  55. McLachlan GJ: Discriminant analysis and statistical pattern recognition. 2004, Wiley series in Probability and Statistics-Interscience, Wiley

    Google Scholar 

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The authors wish to thank Dr. M. Falconi and M. Valentini for their assistance in the sample collection and classification.

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Correspondence to Marcelo Enrique Conti.

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MEC conceived of the study and, together with MBT, participated in its design and drafted the manuscript. MEC, MBT and JS coordinated the sampling protocols and the whole analytical procedures. DC participated in the design and performed the statistical analysis. This project was based on the ideas and under the guidance and consultation of MEC, MBT and LC. All authors read and approved the final manuscript.

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Conti, M.E., Stripeikis, J., Campanella, L. et al. Characterization of Italian honeys (Marche Region) on the basis of their mineral content and some typical quality parameters. Chemistry Central Journal 1, 14 (2007).

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