Table 5 Determination of IS in real plasma samples by developed method and evaluation the accuracy of the method by standard addition method
Sample | Fluorescence intensity | Concentration (µg.mL− 1) | Recovery% |
|---|---|---|---|
A | 146 | 46 | - |
A + 30 (µg.mL− 1) | 234 | 76 | 100 |
A + 80 (µg.mL− 1) | 400 | 134 | 110 |
B | 189 | 61 | - |
B + 30 (µg.mL− 1) | 291 | 96 | 117 |
B + 80 (µg.mL− 1) | 470 | 158 | 98 |