Green chemometric-assisted UV-spectrophotometric methods for the determination of favipiravir, cefixime and moxifloxacin hydrochloride as an effective therapeutic combination for COVID-19; application in pharmaceutical form and spiked human plasma

Table 5 Assay validation sheet of FPV, CEF and MFX by the proposed models

Validation parameters	PLS			GAPLS
Validation parameters	FPV	CEF	MFX	FPV	CEF	MFX
Wavelength (nm)	200–400	200–400	200–400	200–400	200–400	200–400
Linearity range (μg/mL)	1–15	2–15	1–10	1–15	1–15	0.5–10
Slope^a	0.993	1.005	1.001	0.992	1.013	0.999
Intercept^a	0.039	− 0.030	− 0.008	0.045	-0.069	0.007
Coefficient of determination (r²)^a	0.9969	0.9945	0.9988	0.9975	0.9960	0.9996
LOD (μg/mL)^b	0.291	0.383	0.177	0.261	0.323	0.104
LOQ (μg/mL)^b	0.883	1.160	0.536	0.789	0.979	0.314
RMSEC^c	0.089	0.097	0.054	0.080	0.084	0.032
RMSEP^d	0.137	0.173	0.118	0.133	0.131	0.097
RRMSEP^e	2.741	3.458	2.356	2.205	2.904	2.205
BCMSEP^f	0.014	0.029	0.013	0.012	0.021	0.012
RMSECV^g	0.110	0.160	0.111	0.091	0.106	0.045
Accuracy (% R)^h	99.49	100.10	100.12	100.59	98.95	99.49
Precision (% RSD)ⁱ
Repeatability	1.483	1.281	1.452	1.197	1.260	1.184
Intermediate precision	1.141	1.766	1.388	1.691	1.474	1.427

^aData of the straight line plotted between predicted concentrations versus actual concentrations of the calibration set
^bThe LOD and LOQ calculations are based on the net analyte signals
^cRoot-mean-square error of calibration
^dRoot-mean-square error of prediction
^eRelative root-mean-square error of prediction
^fBias-corrected mean square error of prediction
^gRoot mean square error of cross-validation
^hAverage of nine determinations (three concentrations repeated three times)
ⁱ%RSD of nine determinations (three concentrations repeated three times)

ISSN: 2661-801X