Table 7 Robustness of the developed method at the change of flow rate and mobile phase for the Bromoxynil and MCPA
(a) Robustness of the developed method at the change of flow rate and mobile phase for the Bromoxynil | ||||||
|---|---|---|---|---|---|---|
Sample No | Change of Flow Rate | Change of Mobile Phase | ||||
Peak area at 1.3 mL/min | Peak area at 1.5 mL/min | Peak area at 1.7 mL/min | Methanol: Water 95: 05 | Methanol: Water 90: 10 | Methanol: Water 85: 15 | |
01 | 16899261 | 14648612 | 13095952 | 11794631 | 14648612 | 19704492 |
02 | 16893790 | 14660667 | 13112501 | 11793598 | 14660667 | 19731947 |
03 | 16880153 | 14661086 | 13109821 | 11759541 | 14661086 | 19757886 |
04 | 16901669 | 14651265 | 13109991 | 11777437 | 14651265 | 19760224 |
05 | 16927612 | 14615510 | 13108926 | 11726960 | 14615510 | 19762280 |
Mean | 16900497 | 14647428 | 13107438 | 11770433 | 14647428 | 19743366 |
Standard deviation | 17299 | 18687 | 6557 | 28195 | 18687 | 24972 |
% RSD | 0.10% | 0.13% | 0.05% | 0.24% | 0.13% | 0.13% |
(b) Robustness of the developed method at the change of flow rate and mobile phase for the MCPA | ||||||
|---|---|---|---|---|---|---|
Sample No | Change of Flow Rate | Change of Mobile Phase | ||||
Peak area at 1.3 mL/min | Peak area at 1.5 mL/min | Peak area at 1.7 mL/min | Methanol: Water 95: 05 | Methanol: Water 90: 10 | Methanol: Water 85: 15 | |
01 | 14290800 | 12455385 | 11060939 | 9951291 | 12455385 | 16818457 |
02 | 14279376 | 12380559 | 11058719 | 9950908 | 12380559 | 16843060 |
03 | 14267751 | 12381573 | 11057852 | 9921681 | 12381573 | 16875223 |
04 | 14294707 | 12469138 | 11051754 | 9910312 | 12469138 | 16883146 |
05 | 14342259 | 12459192 | 11047533 | 9899583 | 12459192 | 16904524 |
Mean | 14294979 | 12429169 | 11055359 | 9926755 | 12429169 | 16864882 |
Standard deviation | 28451 | 44200 | 5544 | 23557 | 44200 | 34072 |
% RSD | 0.20% | 0.36% | 0.05% | 0.24% | 0.36% | 0.20% |