Parameters | TLC | Reference range [32] | HPLC | Reference range [7] |
---|---|---|---|---|
Resolution (RS) | Rs (SAL-EXP)= 1.53 Rs (EXP-GUF)= 1.54 Rs ( GUF-GUL)= 4.27 Rs ( GUF-BR)= 1.88 | ≥ 1.5 | Rs (SAL-GUF)= 1.6 Rs (GUF-GUL)= 1.53 Rs ( GUL-EXP)= 2.14 Rs ( EXP-BR)= 3.8 | ≥ 1.5 |
Selectivity (α) | α (SAL-EXP)= 2.42 α (EXP-GUF)= 1.58 α ( GUF-GUL)= 2.96 α ( GUF-BR) = 1.20 | ≥ 1 | α (SAL-EXP)= 1.52 α (EXP-GUF)= 1.28 α ( GUF-GUL)= 1.33 α ( GUF-BR) = 1.84 | ≥ 1 |
Capacity Factor (K’) | K’ (SAL)= 19.00 K’ (GUF)= 2.44 K’ ( GUL)= 0.33 K’ ( BR)= 0.10 | > 0.1 | K’ (SAL) = 1.80 K’ (GUF) = 2.60 K’ ( GUL) = 3.36 K’ ( BR)= 6.00 | 1–10 |
Tailing Factor (T) | T (SAL)=1.00 T (GUF)=1.10 T( GUL)=1.05 T ( BR)=1.02 | > 1.5 | T (SAL) = 1.20 T (GUF) = 1.13 T( GUL) = 1.05 T ( BR)= 1.12 | 1–1.5 |
Number of theoretical plates (N) | – | – | N (SAL) =1443 N (GUF) =1817 N( GUL) =2562 N ( BR)=2946 | The higher the number, the more efficient separation |
Height Equivalent to a Theoretical Plate (mm) (HETP) | – | – | N (SAL) =0.17 N (GUF) =0.14 N( GUL) =0.09 N ( BR)=0.08 | The lower the value, the more efficient separation |