Ionic liquid-based ultrasonic-assisted extraction coupled with HPLC to analyze isoquercitrin, trifolin and afzelin in Amygdalus persica L. flowers

Table 4 ANOVA for the fitted quadratic polynomial model for optimization of extraction parameters

Source	Sum of squares	df	Mean square	F value	P value
Model	33.91	14	2.42	9.27	< 0.0001
A	6.60	1	6.60	25.28	0.0002
B	1.18	1	1.18	4.54	0.0514
C	3.99	1	3.99	15.28	0.0016
D	7.73	1	7.73	29.59	< 0.0001
AB	0.23	1	0.23	0.86	0.3684
AC	0.013	1	0.013	0.051	0.8252
AD	0.69	1	0.69	2.64	0.1266
BC	1.80	1	1.80	6.88	0.0201
BD	0.27	1	0.27	1.04	0.3262
CD	0.19	1	0.19	0.72	0.4090
A²	0.18	1	0.18	0.67	0.4254
B²	7.07	1	7.07	27.07	0.0001
C²	1.95	1	1.95	7.45	0.0163
D²	0.81	1	0.81	3.10	0.0999
Residual	3.66	14	0.26
Lack of fit	1.94	10	0.19	0.45	0.8598
Pure error	1.72	4	0.43
Cor total	37.57	28

ISSN: 2661-801X