Spectrum subtraction as a complementary method for six resolution techniques resolving overlapping spectra; application to multicomponent veterinary formulation with greenness and whiteness assessment

Table 5 Results of ANOVA (single factor) for comparison of the proposed methods and the reported methods for determination of TCB and LVM in pure powder form

	Source of Variation	Sum of squares	Degree of freedom	Mean square	F	F crit
TCB^a [5]	Between groups	3.248	7	0.464	0.422	2.249
	Within groups	43.963	40	1.099
	Total	47.211	47
LVM^b [22]	Between groups	3.379	3	1.12	0.797	3.098
	Within groups	28.24	20	1.41
	Total	31.62	23
Comparison with the reported method for determining TCB and LVM in Eradex Forte^® Suspension
TCB^c [8]	Between groups	12.928	11	1.18	0.433	2.22
	Within groups	65.085	24	2.71
	Total	78.013	35
LVM^c [8]	Between groups	17.356	7	2.48	0.93	2.66
	Within groups	42.708	16	2.67
	Total	60.06	23

^a Spectrophotometric method using 0.1 M methanolic HCl as a solvent at wavelength of 305 nm
^b Spectrophotometric method using 0.1N HCl as a solvent at wavelength of 216 nm
^c Principal component regression (PCR) and partial least squares (PLS) chemometric methods

ISSN: 2661-801X