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Table 2 Assay results of MET, EMP, and LIN in their pharmaceutical tablets using the proposed MEKC method

From: Green and white MEKC for determination of different anti-diabetic binary mixtures and their triple-combo pill

 

MEKC Method

Reference Method*

 

MET

EMP

LIN

MET

EMP

LIN

MET/EMP/LIN (Mixture I)a

Mean % recovery ± SD

RSD %

Er %

t e

F e

100.27 ± 0.16

0.16

0.27

1.71

1.12

99.84 ± 0.68

0.68

−0.16

0.53

3.04

100.44 ± 0.71

0.71

0.44

2.22

3.91

99.96 ± 0.025

0.025

−0.04

99.65 ± 0.39

0.39

−0.35

99.65 ± 0.36

0.36

−0.35

MET/EMP (Mixture II)b

Mean % recovery ± SD

RSD %

Er %

t e

F e

100.04 ± 0.59

0.59

0.04

0.80

5.15

99.60 ± 0.68

0.68

−0.40

2.10

4.00

 

99.81 ± 0.26

0.26

−0.19

98.88 ± 0.34

0.34

−1.12

 

MET/LIN (Mixture III)c

Mean % recovery ± SD

RSD %

Er %

t e

F e

100.52 ± 1.50

1.49

0.52

0.12

5.01

 

100.46 ± 1.96

1.95

0.46

0.07

1.85

100.17 ± 0.67

0.67

0.17

 

100.54 ± 1.44

1.43

0.54

EMP/LIN (Mixture IV)d

Mean % recovery ± SD

RSD %

Er %

t e

F e

 

99.84 ± 0.80

0.80

−0.16

2.24

1.91

101.10 ± 0.75

0.74

1.10

2.15

3.16

 

100.83 ± 0.58

0.57

0.83

100.28 ± 0.42

0.42

0.28

  1. *The reference methods used were HPLC methods for Mix I (7) and Mix II (13), CE for Mix III (22) and spectrophotometric method for Mix IV (21)
  2. aLabeled to contain 12.5 EMP, 2.5 LIN and 500 METµg.mL−1
  3. bLabeled to contain 12.5 EMP and 500 MET µg. mL−1
  4. cLabeled to contain 2.5 LIN and 500 MET µg. mL−
  5. dLabeled to contain 25 LIN and 50 EMP µg. mL−
  6. eTheoretical values at 95% confidence level (n = 5) for t and F are 2.31 and 6.39, respectively