Development and validation of an eco-friendly HPLC–UV method for determination of atorvastatin and vitamin D3 in pure form and pharmaceutical formulation

Table 6 Comparison of the proposed analytical method to the reported HPLC methods

	Proposed green HPLC method	Reported method [30]	Reported method [31]
Technique	Green HPLC–UV	RP-HPLC–UV	RP-HPLC–UV
Linearity range (µg/ml) Atorvastatin calcium	5–40	50–500	8–28
Vitamin D₃	1–8	0.13–1.25	2–7
Organic Solvent	0.1% ortho-phosphoric acid (OPA) (pH = 2.16), ethanol	Methanol, Acetonitrile (50%:50%) pH adjusted to 4 with 0.1% formic acid	Acetonitrile, Methanol (75%: 25%) pH adjusted to 3.5 adjusted with phosphoric acid
Run time (min)	12	15	20
Column	Symmetry column C₁₈ (100 × 4.6 mm, 3.5 µm)	Enable C₈-column (15 cm × 4.6 mm, 5 μm)	Phenomenex, Luna C₁₈ (250 × 4.6 mm, 5 μm)
GAPI assessment
AGREE assessment

ISSN: 2661-801X