Fig. 4
Chromatogram of 20 µl injection of standard solutions of atorvastatin calcium (0.1 mg/ml) and vitamin D3 (0.1 mg/ml) using 0.1% orthophosphoric acid and ethanol as the mobile phase in gradient matter at a flow rate 1 ml/min with column temperature was maintained at 40 °C and detection at 246 and 264 nm for atorvastatin calcium and vitamin D3 respectively