Table 1 Regression and validation data for quantitative analysis of FPV and RDV by the proposed methods

Wavelength (nm)

222 & 256

247 & 271

228

251.2

Linearity range (µg/mL)

2–24

2–24

2–24

2–24

Slope

0.2571

0.0784

2.7023

2.7526

Intercept

− 0.0023

− 0.0004

− 0.1100

− 0.0123

Coefficient of determination (r²)

0.9997

0.9998

0.9997

0.9998

LOD (µg/mL)

0.390

0.336

0.402

0.331

LOQ (µg/mL)

1.182

1.018

1.217

1.002

Accuracy (%R)^a

100.32

99.89

100.46

99.67

Repeatability precision (RSD)^b

0.555

0.695

0.492

0.672

Intermediate precision (RSD)^b

0.701

0.815

0.716

0.850