Three techniques for the determination of perindopril through derivatization with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole

Table 2 Assay outcomes for PRD determination in pure form by the official and proposed methods

Parameter	Method I			Method II			Method III			Official method [3] % Found
Parameter	The amount taken (µg mL⁻¹)	Amount found (µg mL⁻¹)	% Found	The amount taken (µg mL⁻¹)	Amount found (µg mL⁻¹)	% Found	The amount taken (µg mL⁻¹)	Amount found (µg mL⁻¹)	% Found	Official method [3] % Found
	5.0	4.903	98.05	0.5	0.504	100.69	1.0	0.981	98.14	99.03
	10.0	9.871	98.71	1.0	0.997	99.70	3.0	3.024	100.8	98.74
	20.0	19.800	99.00	2.0	2.029	101.48	5.0	5.013	100.25	99.4
	40.0	39.420	98.55	4.0	4.045	101.12	7.0	6.975	99.64
	60.0	59.466	99.11	6.0	6.060	101.00	10.0	10.016	100.16
X`			98.68			100.80			99.85	99.06
± S.D			± 0.42			± 0.68			± 0.91	± 0.33
t			1.30			1.94			1.19
F			1.60			4.17			9.39

Each result is the average of three separate determinations
The tabulated values of t and F are (2.44) & (19.24), respectively, at p = 0.05 [38]

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