Classical versus chemometrics tools for spectrophotometric determination of fluocinolone acetonide, ciprofloxacin HCl and ciprofloxacin impurity-A in their ternary mixture

Table 3 Determination of CIP and FLU in its otic vial dosage form and application of standard addition technique using the suggested methods

Otovel® otic vials 0.75 mg CIP/ 0.0625 mg FLU in each 0.25 mL lot no. 20DE7		CIP						Otovel® otic vials 0.75 mg CIP/ 0.0625 mg FLU in each 0.25 mL lot no. 20DE7		FLU
		D¹	RD	DR	MC	PLS	ANN			DDRD	PLS	ANN
Mean^a		99.61	100.97	99.64	100.08	100.09	99.43	Mean^a		100.26	98.65	100.41
RSD%		1.637	1.388	1.659	1.309	1.027	1.159	RSD%		1.205	1.281	1.042
Standard addition technique
CIP								FLU
Taken (μg/mL)	Added (μg/mL)	Recovery %						Taken (μg/mL)	Added (μg/mL)	Recovery %
9.4	5.0	99.26	99.32	99.98	101.91	98.82	98.72	0.78	0.4	99.92	99.28	101.21
	10.0	101.11	101.56	101.38	99.49	101.32	100.52		0.8	100.20	100.12	100.94
	20.0	101.48	98.02	99.87	100.35	101.01	99.02		1.6	101.81	98.04	98.85
Mean		100.62	99.63	100.41	100.58	100.38	99.42	Mean		100.64	99.14	100.33
RSD%		1.183	1.799	0.840	1.218	1.358	0.970	RSD%		1.016	1.055	1.287

ISSN: 2661-801X