Green quality by design HPLC approach for the simultaneous determination of Bilastine and Montelukast

BMC Chemistry

Table 2 Parameters of system suitability of the proposed HPLC method for the determination of BIL and MNT

Parameter	UV chromatographic value		Fluorescence chromatographic value
Parameter	BIL	MNT	MNT
No theoretical plates, N	1443	1093	1103
Capacity factor, km	0.26	0.95	0.9
Selectivity factor, α	3.65
Resolution, R_s	3.2
Retention time (t_R), min	2.4	3.7	3.6
Tailing factor (T)	1.45	1.27	1.27

Where: Number of theoretical plates (N) = \(5.54\left( {\tfrac{{{\text{tR}}}}{{\text{Wh/2}}}} \right)^{2}\)
Resolution (R_s) = \(\tfrac{{2\Delta {\text{tR}}}}{{{\text{W}}1\; + \;{\text{W}}2}}\)
Capacity factor `) = \(\frac{{\mathrm{t}}_{\mathrm{R}}-{\mathrm{t}}_{\mathrm{m}}}{{\mathrm{t}}_{\mathrm{m}}}\)
The electivity (α) = \(\frac{{\mathrm{k}`}_{2}}{{\mathrm{k}`}_{1}}\)
Tailing factor (T) = \(\frac{{W}_{0.05}}{2d}\)

ISSN: 2661-801X