Table 1 23 Experimental factorial designs and their dependent responses for RP-HPLC–UV separation of BIL and MNT mixture
Design order | Experimental factorial design | Dependent responses | |||||||
|---|---|---|---|---|---|---|---|---|---|
Std order | Run order | %MeOH (A) | %ACN (B) | pH (C) | Flow rate (D) | T2 of MNT | NTP 2 of MNT | Run time | Rs |
5 | 1 | 85 | 3 | 6 | 0.8 | 1.42 | 429 | 6.1 | 6.1 |
6 | 2 | 92 | 3 | 6 | 0.8 | 1.3 | 1642 | 3.5 | 3.5 |
14 | 3 | 92 | 3 | 6 | 1.2 | 1.3 | 893 | 3.6 | 3.6 |
15 | 4 | 85 | 6 | 6 | 1.2 | 1.7 | 661 | 5 | 5 |
4 | 5 | 92 | 6 | 3 | 0.8 | 1.2 | 3226 | 7.3 | 7.3 |
9 | 6 | 85 | 3 | 3 | 1.2 | 1.65 | 165 | 2.9 | 2.9 |
1 | 8 | 85 | 3 | 3 | 0.8 | 1.9 | 151 | 2.81 | 2.81 |
2 | 9 | 92 | 3 | 3 | 0.8 | 1.25 | 3341 | 10 | 10 |
8 | 10 | 92 | 6 | 6 | 0.8 | 1.12 | 1261 | 2.8 | 2.8 |
16 | 11 | 92 | 6 | 6 | 1.2 | 1.28 | 1135 | 2.56 | 2.56 |
3 | 12 | 85 | 6 | 3 | 0.8 | 1.56 | 328 | 3.85 | 3.85 |
13 | 14 | 85 | 3 | 6 | 1.2 | 1.41 | 1417 | 6.48 | 6.48 |
11 | 15 | 85 | 6 | 3 | 1.2 | 1.36 | 591 | 5.20 | 5.20 |
12 | 16 | 92 | 6 | 3 | 1.2 | 1.20 | 2159 | 5.90 | 5.90 |
10 | 17 | 92 | 3 | 3 | 1.2 | 1.20 | 2367 | 8.40 | 8.40 |
7 | 18 | 85 | 6 | 6 | 0.8 | 1.40 | 918 | 5.90 | 5.90 |