Table 8 The penalty points of the proposed methods according to the analytical Eco-Scale
Reagents/ Instruments | Penalty points | ||||||
|---|---|---|---|---|---|---|---|
Reagent | Number of pictograms | Word sign | Reagent, penalty points | Proposed method | Reported method [7] | Reported method [8] | Reported method [9] |
Methanol | 3 | Danger | 6 | 6 × 1 = 6 | 6 × 2 = 12 | 6 × 2 = 12 | |
Acetonitrile | 2 | Danger | 4 | 4 × 2 = 8 | 4 × 2 = 8 | ||
n- propanol | 2 | Danger | 4 | 2 × 4 = 8 | |||
Glacial acetic acid | 2 | Danger | 4 | 2 × 4 = 8 | |||
TEA | 3 | 3 | 3 × 1 = 3 | ||||
o-phosphoric acid | 2 | 2 | 2 × 1 = 2 | 2 × 2 = 4 | 2 × 2 = 4 | 2 × 1 = 2 | |
Sodium acetate | 1 | Irritant | 2 | 2 × 2 = 4 | |||
HPLC | |||||||
Occupational hazard, Analytical process hermitization | 0 | 0 | 0 | 0 | |||
Waste Flow rate x run time | 1–10 = 3 > 10 = 5 | 3 | 5 | 5 | 5 | ||
Total penalty points | 16 | 23 | 29 | 31 | |||
Analytical Eco- Scale total score | 84 | 77 | 71 | 69 | |||