Table 5 Accuracy of the proposed HPLC method using mobile phase n-hexane: ethanol: DEA (60: 40: 0.1 by volume) for determination of bisoprolol, carvedilol and atenolol enantiomers
Bisoprolol | Carvedilol | Atenolol | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Taken \(\mathrm{\mu g}/\mathrm{mL}\) | Found \(\mathrm{\mu g}/\mathrm{mL}\) enant.1 | Recovery % enant.1 | Found \(\mathrm{\mu g}/\mathrm{mL}\) enant.2 | Recovery % enant.2 | Taken \(\mathrm{\mu g}/\mathrm{mL}\) | Found \(\mathrm{\mu g}/\mathrm{mL}\) enant.1 | Recovery % enant.1 | Found \(\mathrm{\mu g}/\mathrm{mL}\) enant.2 | Recovery % enant.2 | Taken \(\mathrm{\mu g}/\mathrm{mL}\) | Found \(\mathrm{\mu g}/\mathrm{mL}\) enant.1 | Recovery % enant.1 | Found \(\mathrm{\mu g}/\mathrm{mL}\) enant.2 | Recovery % enant.2 |
50.00 | 49.80 | 99.60 | 49.86 | 99.72 | 20.00 | 20.30 | 101.50 | 20.06 | 100.30 | 50.00 | 50.65 | 101.30 | 49.66 | 99.32 |
100.00 | 98.93 | 99.38 | 99.38 | 99.38 | 40.00 | 40.16 | 100.40 | 40.27 | 100.68 | 100.00 | 100.61 | 100.61 | 101.52 | 101.52 |
150.00 | 152.00 | 101.33 | 150.41 | 100.27 | 60.00 | 59.27 | 98.78 | 59.84 | 99.73 | 150.00 | 149.34 | 99.56 | 150.23 | 100.15 |
200.00 | 198.66 | 99.33 | 199.12 | 99.56 | 80.00 | 79.18 | 98.98 | 78.79 | 98.49 | 200.00 | 198.86 | 99.43 | 196.65 | 98.33 |
250.00 | 250.45 | 100.18 | 251.16 | 100.46 | 100.00 | 99.71 | 99.71 | 100.01 | 100.01 | 250.00 | 249.67 | 99.87 | 248.57 | 99.43 |
300.00 | 300.60 | 100.20 | 300.47 | 100.16 | 120.00 | 120.63 | 100.53 | 121.57 | 101.31 | 300.00 | 303.98 | 101.33 | 303.18 | 101.06 |
Mean | 99.93 | 99.93 | 99.98 | 100.09 | 100.35 | 99.97 | ||||||||
R.S.D | 0.942 | 0.432 | 1.030 | 0.956 | 0.849 | 1.185 | ||||||||