Enantioseparation, quantification, molecular docking and molecular dynamics study of five β-adrenergic blockers on Lux-Cellulose-2 column

Table 4 Regression and validation parameters of the proposed HPLC method for quantitative determination of Metoprolol, Nebivolol, carvedilol and atenolol enantiomers using mobile phase n-hexane: ethanol: DEA (75: 25: 0.1by volume)

Parameters	Metoprolol		Nebivolol		Carvedilol		Atenolol
Parameters	Enant.1	Enant.2	Enant.1	Enant.2	Enant.1	Enant.2	Enant.1	Enant.2
Linearity
Slope (Au-Sec.ml/µg)	3.2903	3.3722	1.0659	1.1315	11.3710	11.4470	3.8646	3.6325
Intercept (Au-Sec)	− 3.3282	− 6.3580	− 4.4288	− 2.0739	− 13.5980	− 17.7210	17.4170	25.2190
Correlation coefficient r	0.9996	0.9995	0.9995	0.9997	0.9996	0.9996	0.9995	0.9995
Range (\(\upmu \)g/ml)	50–250	50–250	50–250	50–250	20–100	20–100	50–250	50–250
Accuracy (Mean + R.S.D.%)	99.96 + 1.022	100.21 + 0.867	99.90 + 0.587	100.40 + 0.898	100.29 + 1.088	99.94 + 1.000	100.29 + 0.898	100.12 + 1.405
*Robustness (RSD %)	0.996	0.853	0.531	0.832	0.992	0.997	0.845	0.998
**Robustness (RSD %)	0.931	0.792	0.622	0.774	0.832	0.851	0.641	0.826
Precision (RSD %)
Repeatabilty^a	0.821	0.612	0.481	0.765	0.851	0.805	0.733	0.914
Reproducibility^b	0.933	0.693	0.531	0.801	0.942	0.912	0.799	0.982
LOD^c (\(\upmu \)g/mL)	6.7	6.7	6.7	6.7	2.7	2.7	6.7	6.7
LOQ^c (\(\upmu \)g/mL)	20	20	20	20	8	8	20	20

^aIntra-day (n = 3), average of three concentrations of each drug, repeated three times within the same day
^bInter-day (n = 3), average of three concentrations of each drug repeated three times in three consecutive days
^cLOD = 3.3 (SD/S), LOQ = 10(SD/S), where SD is the standard deviation of regression residuals and S is the slope of calibration curves
*The robustness of the proposed method was investigated by analysis of samples under deliberate change in flow rate ± 0.1 mL/min
**The robustness of the proposed method was investigated by analysis of samples under deliberate change in Temperature ± 0.1 °C

ISSN: 2661-801X