Comparative study of extension area based methods for spectrophotometric determination of desmopressin acetate in the presence of its acid-induced degradation products

Table 2 Determination of intact desmopressin acetate (DPA) in laboratory prepared mixtures with its degradants by the proposed methods

Conc. of DPA (µg/mL)	Conc. of degradants ( g/mL)	% of degradants	Recovery % of Intact DPA
Conc. of DPA (µg/mL)	Conc. of degradants ( g/mL)	% of degradants	First derivative	Ratio derivative	Ratio difference	Mean centering	Dual wavelength
13	1	7.14	101.58	98.39	101.93	100.80	99.02
12	2	14.28	98.77	101.28	99.04	101.03	100.11
10	4	28.57	99.82	99.92	101.47	100.91	99.23
8	6	42.86	100.39	99.40	100.19	99.92	98.01
6	8	57.14	99.01	100.64	98.62	99.73	101.16
4	10	71.43	98.16	108.59 ^a	99.95	100.69	98.14
2	12	85.71	95.23 ^a	114.28 ^a	101.82	106.24 ^a	104.97 ^a
Mean			99.62	99.93	100.43	100.51	99.28
RSD%			1.241	1.117	1.334	0.546	1.209

^aUnder-lined values are out of accepted range and not considered in the calculation of Mean or SD

ISSN: 2661-801X