Parameters | First derivative | Ratio derivative | Ratio difference | Mean centering | Dual wavelength |
---|---|---|---|---|---|
Wavelength | 232.6 nm | 236 nm | 225–277 nm | 236 nm | 237–273 nm |
Calibration range | (1–14 µg /mL) | (1–14 µg /mL) | (1–14 µg /mL) | (1–14 µg /mL) | (1–14 µg /mL) |
Slope | 0.00163 | 0.0374 | 0.7191 | 0.6482 | 0.0298 |
Intercept | 0.00064 | 0.0122 | 0.0652 | 0.1753 | 0.0032 |
Correlation coefficient | 0.9998 | 0.9997 | 0.9997 | 0.9998 | 0.9997 |
LOD | 0.304 | 0.274 | 0.167 | 0.248 | 0.199 |
LOQ | 0.920 | 0.829 | 0.506 | 0.751 | 0.604 |