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Fig. 4 | BMC Chemistry

Fig. 4

From: Development and validation of a novel Spectrofluorimetric method of oral anticoagulant Edoxaban via derivatization with 9-fluorenyl methyl chloroformate: green assessment of the method by Eco-Scale and ComplexGAPI

Fig. 4

Optimization of experimental conditions for EDTM reaction with FMOC-Cl each factor was measured three times including: a The influence of buffer pH on the formation of EDTM-FMOC after derivatizing of EDTM (15 ng/mL) with FMOC-Cl. b The influence of buffer volume on the formation of EDTM-FMOC between EDTM (15 ng/mL) and FMOC-Cl. c The influence of FMOC-Cl volume on the formation of EDTM-FMOC between EDTM (15 ng/mL) and FMOC-Cl. d The influence of volume of acetonitrile on the formation of EDTM-FMOC between EDTM (15 ng/mL) and FMOC-Cl. e The influence of heating temperature on the formation of EDTM-FMOC between EDTM (15 ng/mL) and FMOC-Cl. f The influence t of heating time on the formation of EDTM-FMOC between EDTM (15 ng/mL) and FMOC-Cl

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BMC Chemistry

ISSN: 2661-801X

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