Fig. 4

Procaspase-3 expression in HepG2 cells treated with DMSO or 2, 5 and 10 μM concentrations of compound 4c for 48 h. β-actin was used as internal control. a The corresponding protein levels in the cropped gel were assessed using densitometry. Samples derived from the same experiment were run in two different gels simultaneously, and were processed in parallel. b Relative expression of procaspase-3 to β-actin was measured. Each value represents the mean ± SEM of three independent experiments. One-way ANOVA analysis with Tukey post test was performed