Skip to main content

Table 1 Regression and validation data for the determination of FEX and PSE by the proposed method

From: Application of green first derivative synchronous spectrofluorometric method for quantitative analysis of fexofenadine hydrochloride and pseudoephedrine hydrochloride in pharmaceutical preparation and spiked human plasma

Parameters FEX PSE
Wavelength (nm) 286 294
Linearity range (ng/mL) 100–1500 50–1000
LOD (ng/mL) 24.15 12.88
LOQ (ng/mL) 73.49 39.21
Slope 0.0377 0.0623
Intercept 9.6551 8.8644
Coefficient of determination (r2) 0.9995 0.9994
Accuracy (%R)a 99.13 98.46
Precision (RSD)b
 Repeatability 1.369 0.493
 Intermediate precision 1.012 1.207
Robustness (%R ± RSD)
 Δλ (± 1 nm.) 100.63 ± 0.926 99.20 ± 1.017
 pH (± 0.1) 99.98 ± 1.066 99.82 ± 1.314
 Buffer volume (± 0.1 mL) 100.89 ± 0.878 100.12 ± 0.950
  1. aAverage of nine determinations (three concentrations repeated three times)
  2. bRSD of nine determinations (three concentrations repeated three times)