Table 1 Regression and validation data for quantitative analysis of FSD and TDL by the proposed methods

Wavelength (nm)

230.80

292

218.80

289.60

Linearity range (µg/mL)

10–140

3–40

10–140

3–40

Slope

0.0145

0.0822

0.0976

0.8662

Intercept

− 0.0177

0.0223

-0.0574

0.2200

Coefficient of determination (r²)

0.9997

0.9996

0.9998

0.9996

LOD (µg/mL)

2.406

0.876

2.229

0.815

LOQ (µg/mL)

7.292

2.654

6.755

2.470

Accuracy (%R)^a

99.57

99.64

99.52

99.71

Repeatability precision (RSD)^b

1.192

0.959

0.444

0.650

Intermediate precision (RSD)^b

1.198

0.963

0.446

0.652

Robustness (%R ± RSD) − Wavelength (± 0.2)

99.78 ± 0.989

100.17 ± 0.731

100.05 ± 0.601

99.56 ± 1.052