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Table 1 Analytical performance data for the adopted method for the estimation of TIM and TFL

From: Green HPLC method with time programming for the determination of the co-formulated eye drops of tafluprost and timolol in their challengeable ratio

Parameter

Proposed method

TFL

TIM

Detection wavelength (nm)

220

254

Linearity range

(µg mL−1)

0.6–45

50–2000

Intercept (a)

0.0004

− 0.0495

Slope (b)

0.0159

0.0018

n

9

8

Correlation coefficient (r)

0.9999

0.9999

Standard deviation of residuals (Sy/x)

2.0 × 10–3

15.6 × 10–3

Standard deviation of intercept (Sa)

9.0 × 10–4

9.0 × 10–3

Standard deviation of slope (Sb)

4.0 × 10–5

1.0 × 10–5

% Relative standard deviation (% RSD)

0.92

1.11

% Error

0.306

0.394

Limit of detection (LOD)

(µg mL−1)

0.18

16.48

Limit of quantitation (LOQ) (µg mL−1)

0.55

49.94