Development and validation of liquid chromatography method for simultaneous determination of multiclass seven antibiotic residues in chicken tissues

Table 4 Effects of the analytical parameters change performed for robustness evaluation in flow rate, pH of mobile phase and temperature

Compounds	Parameters changed for robustness study
	Flow rate (mL min⁻¹)				pH of mobile phase			Analysis temperature °C
		0.8	1.0	1.2	7.8	8.0	8.2	38	40	42
GENTA	Peak symmetry	0.96	1.00	0.99	0.59	0.47	0.22	0.22	0.90	0.84
	% RSD	1.14	3.33	2.15	1.96	1.96	3.02	2.43	3.10	3.10
	Rt (min)	3.78	3.85	3.65	3.80	3.86	3.88	3.56	3.83	3.79
AMOX	Peak symmetry	0.96	0.94	0.79	0.97	0.78	1.00	0.94	0.39	0.68
	% RSD	3.27	2.54	3.70	0.22	1.24	4.32	2.37	2.88	1.25
	Rt (min)	5.89	5.82	5.90	5.69	5.81	5.76	5.70	5.89	5.79
PEN G	Peak symmetry	0.74	0.25	0.86	0.85	0.64	0.94	0.95	1.59	0.65
	% RSD	4.57	3.26	2.37	2.15	1.28	0.26	1.24	1.24	0.27
	Rt (min)	11.65	11.25	11.28	11.98	11.36	11.98	11.22	11.21	11.14
AMPI	Peak symmetry	0.52	1.00	0.50	1.00	1.26	0.78	1.46	0.68	0.63
	% RSD	2.43	3.27	1.25	5.32	4.24	6.32	1.24	4.24	1.98
	Rt (min)	7.37	7.38	7.46	7.21	7.31	7.21	7.97	7.33	7.30
ERYTHRO	Peak symmetry	1.25	0.95	0.95	1.15	1.09	1.74	0.22	0.84	0.68
	% RSD	1.24	1.42	1.98	2.37	4.37	4.98	2.40	1.87	1.79
	Rt (min)	4.75	4.75	4.80	4.72	4.71	4.75	4.69	4.76	4.60
SULFA	Peak symmetry	0.99	0.99	1.00	1.00	0.61	0.67	0.42	0.90	0.47
	% RSD	3.02	2.67	4.14	2.43	3.10	3.10	3.27	1.24	0.24
	Rt (min)	8.80	8.82	8.86	8.81	8.75	8.12	8.54	8.85	8.13
CIPRO	Peak symmetry	0.91	0.59	0.22	1.04	1.00	0.91	0.91	0.59	0.22
	% RSD	3.04	1.32	3.26	2.65	3.12	2.37	1.24	3.26	3.01
	Rt (min)	6.50	6.55	6.24	6.56	6.70	6.28	6.45	6.12	6.25

ISSN: 2661-801X