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Table 4 Effects of the analytical parameters change performed for robustness evaluation in flow rate, pH of mobile phase and temperature

From: Development and validation of liquid chromatography method for simultaneous determination of multiclass seven antibiotic residues in chicken tissues

Compounds Parameters changed for robustness study
Flow rate (mL min−1) pH of mobile phase Analysis temperature °C
  0.8 1.0 1.2 7.8 8.0 8.2 38 40 42
GENTA Peak symmetry 0.96 1.00 0.99 0.59 0.47 0.22 0.22 0.90 0.84
% RSD 1.14 3.33 2.15 1.96 1.96 3.02 2.43 3.10 3.10
Rt (min) 3.78 3.85 3.65 3.80 3.86 3.88 3.56 3.83 3.79
AMOX Peak symmetry 0.96 0.94 0.79 0.97 0.78 1.00 0.94 0.39 0.68
% RSD 3.27 2.54 3.70 0.22 1.24 4.32 2.37 2.88 1.25
Rt (min) 5.89 5.82 5.90 5.69 5.81 5.76 5.70 5.89 5.79
PEN G Peak symmetry 0.74 0.25 0.86 0.85 0.64 0.94 0.95 1.59 0.65
% RSD 4.57 3.26 2.37 2.15 1.28 0.26 1.24 1.24 0.27
Rt (min) 11.65 11.25 11.28 11.98 11.36 11.98 11.22 11.21 11.14
AMPI Peak symmetry 0.52 1.00 0.50 1.00 1.26 0.78 1.46 0.68 0.63
% RSD 2.43 3.27 1.25 5.32 4.24 6.32 1.24 4.24 1.98
Rt (min) 7.37 7.38 7.46 7.21 7.31 7.21 7.97 7.33 7.30
ERYTHRO Peak symmetry 1.25 0.95 0.95 1.15 1.09 1.74 0.22 0.84 0.68
% RSD 1.24 1.42 1.98 2.37 4.37 4.98 2.40 1.87 1.79
Rt (min) 4.75 4.75 4.80 4.72 4.71 4.75 4.69 4.76 4.60
SULFA Peak symmetry 0.99 0.99 1.00 1.00 0.61 0.67 0.42 0.90 0.47
% RSD 3.02 2.67 4.14 2.43 3.10 3.10 3.27 1.24 0.24
Rt (min) 8.80 8.82 8.86 8.81 8.75 8.12 8.54 8.85 8.13
CIPRO Peak symmetry 0.91 0.59 0.22 1.04 1.00 0.91 0.91 0.59 0.22
% RSD 3.04 1.32 3.26 2.65 3.12 2.37 1.24 3.26 3.01
Rt (min) 6.50 6.55 6.24 6.56 6.70 6.28 6.45 6.12 6.25