Table 3 Comparison of control points by Charm II protocol, cut-off points and technical threshold values calculated according to the EU guideline [26, 27]
Antimicrobial family | Compound | Spiked concentration (μg/kg) | B average response of blank samples | Calculated T value as per EU guideline T = B − 1.64 * SDb [26, 27] | Calculated Fm value as per EU guideline Fm = M + 1.64 * SDs [26, 27] | Calculated control point CP as per Charm II assay |
|---|---|---|---|---|---|---|
Tetracyclines | Tetracycline | 25 | 2958 | 2776 | 816 | 1514 |
Chlortetracycline | 25 | 3050 | 2780 | 1417 | ||
Oxytetracycline | 100 | 2891 | 2579 | 1427 | ||
Macrolides | Erythromycin A | 100 | 2814 | 2564 | 1904 | 2118 |
Tilmicosin | 100 | 2486 | 2164 | 2002 | ||
Tylosin A | 100 | 2512 | 2115 | 1740 | ||
β-Lactams | Penicillin G | 25 | 2523 | 2176 | 1438 | 1530 |
Ampicillin | 50 | 2455 | 2042 | 1341 | ||
Amoxicillin | 50 | 2702 | 2536 | 1487 | ||
Oxacillin | 300 | 2398 | 2171 | 1478 | ||
Dicloxacillin | 300 | 2524 | 2384 | 1489 | ||
Cloxacillin | 300 | 2500 | 2368 | 1413 | ||
Aminoglycosides | Streptomycin | 25 | 4822 | 3867 | 2592 | 3346 |
Sulfonamides | Sulfamethazine | 25 | 2593 | 1428 | 1379 | 1424 |
Sulfadimethoxine | 25 | 2266 | 1129 | 972 | ||
Sulfamerazine | 25 | 2210 | 1095 | 930 | ||
Sulfadiazine | 25 | 2297 | 1296 | 1184 | ||
Sulfathiazole | 25 | 2266 | 1643 | 1485 |