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Table 11 Specificity and cross reactivity tests with mixed standards of different antimicrobial using the Aminoglycosides kit

From: Primary validation of Charm II tests for the detection of antimicrobial residues in a range of aquaculture fish

Antibiotics used and their respective MRL

Mixed standard and respective spiking level, µg/kg

Fish species

Spiked fish samples cpm

Tetracycline (MRL 100 µg/kg)

Tetracycline spiked at 1000 µg/kg

Cat fish

5742

Cat fish

6286

Penicillin G (MRL 50 µg/kg)

Penicillin G spiked at 500 µg/kg

Cat fish

5780

Cat fish

5776

Sulfamethazine (MRL 100 µg/kg)

Sulfamethazine spiked at 1000 µg/kg

Salmon

5418

Salmon

5700

Salmon

5584

Tilmicosin (50 MRL µg/kg)

Tilmicosin spiked at 500 µg/kg

Trout

5776

Trout

5584

Trout

6155

Trout

5962

Trout

5777

Trout

5800

Trout

5671

Trout

6010

Trout

5699

Trout

5700

Trout

5800

Trout

5810

Trout

5156

Aminoglycosides calculated control point cpm = 3346