Table 2 Summary of the procedures for purification of β-glucosidase from Boju
Step | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Purification (fold) | Activity recovery (%) |
|---|---|---|---|---|---|
Crude extract | 363 | 1.980 | 0.005 | 1 | 100 |
Ammonium sulfate precipitation | 79 | 0.784 | 0.010 | 2.0 | 39.6 |
DEAE-cellulose-52 chromatography | 8.1 | 0.402 | 0.040 | 9.9 | 20.3 |
Sephadex™G-100 gel filtration | 0.7 | 0.087 | 0.124 | 24.8 | 4.3 |