|
Parameter
|
No. of theoretical plates (N)
|
Capacity factor (k′)
|
Tailing factor (Tf)
|
|---|
|
Column (A)
|
Column (B)
|
Column (A)
|
Column (B)
|
Column (A)
|
Column (B)
|
|---|
|
Column temperature °C
|
|
25 °C
|
4354.9
|
1014.2
|
1.147
|
4.638
|
0.475
|
0.629
|
|
40 °C
|
2950.3
|
756.4
|
1.057
|
4.404
|
0.506
|
0.772
|
|
50 °C
|
2620.8
|
677.9
|
0.989
|
4.208
|
0.471
|
0.73
|
|
65 °C
|
1895.1
|
468.9
|
0.871
|
3.792
|
0.468
|
0.679
|
|
pH of mobile phase
|
|
2.5
|
4354.9
|
1014.2
|
1.147
|
4.638
|
0.475
|
0.629
|
|
3.5
|
3981.2
|
1089
|
1.145
|
4.667
|
0.526
|
0.588
|
|
5
|
4617.2
|
1066.9
|
1.145
|
4.667
|
0.455
|
0.572
|
|
Type of organic modifier of Conc 40% (v/v)
|
|
Acetonitrile
|
4390.4
|
1004.5
|
1.236
|
4.667
|
0.446
|
0.679
|
|
Methanol
|
5073.4
|
1214.5
|
1.179
|
4.738
|
0.474
|
0.664
|
|
Ethanol
|
3342
|
925.4
|
1.175
|
4.708
|
0.481
|
0.65
|
|
Ratio organic modifier: mobile phase (Acetonitrile) (v/v)
|
|
40:60
|
4354.9
|
1100.3
|
1.147
|
4.696
|
0.475
|
0.677
|
|
60:40
|
1925.3
|
1004.5
|
1.173
|
4.667
|
0.453
|
0.679
|
|
80:20
|
7501.7
|
1648.5
|
1.187
|
4.75
|
0.468
|
0.647
|
|
90:10
|
8304.6
|
2064.8
|
1.19
|
4.792
|
0.458
|
0.653
|
|
Effect of flow rate (mL/min)
|
|
0.3
| |
1041
| |
8.196
| |
0.816
|
|
0.5
| |
1090.7
| |
4.613
| |
0.613
|
|
1
|
5093.6
| |
1.53
| |
0.287
| |
|
1.2
|
1117.9
| |
1.175
| |
0.465
| |
-
\( {\text{Number of theoretical plates }}\left( {\text{N}} \right)\, = \,5.54\left( {\frac{{{t}_{{R}} }}{{{Wh}/2}} } \right)^{2} \)
- \( {\text{Tailing factor }}\left( {\text{T}} \right)\, = \,\frac{{{W}_{0.5} }}{{2{f}}}{\text{k}^{\prime}} \, = \,{{({\text{tR}} {-}{\text{ t}}0)} \mathord{\left/ {\vphantom {{({\text{tR}} {-}{\text{ t}}0)} {{\text{t}}0}}} \right. \kern-0pt} {{\text{t}}0}} \),
