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Table 2 Optimization of the chromatographic conditions for the determination of SFV by Method 2

From: Two novel UPLC methods utilizing two different analytical columns and different detection approaches for the simultaneous analysis of velpatasvir and sofosbuvir: application to their co-formulated tablet

Parameter

No. of theoretical plates (N)

Capacity factor (k′)

Tailing factor (Tf)

Column (A)

Column (B)

Column (A)

Column (B)

Column (A)

Column (B)

Column temperature °C

 25 °C

4354.9

 1014.2

1.147

4.638 

0.475

0.629 

 40 °C

2950.3

756.4

1.057

4.404

0.506

0.772

 50 °C

2620.8

677.9

0.989

4.208

0.471

0.73

 65 °C

1895.1

468.9

0.871

3.792

0.468

0.679

pH of mobile phase

 2.5

4354.9

1014.2

1.147

4.638

0.475

0.629

 3.5

3981.2

1089

1.145

4.667

0.526

0.588

 5

4617.2

1066.9

1.145

4.667

0.455

0.572

Type of organic modifier of Conc 40% (v/v)

 Acetonitrile

4390.4

1004.5

1.236

4.667

0.446

0.679

 Methanol

5073.4

1214.5

1.179

4.738

0.474

0.664

 Ethanol

3342

925.4

1.175

4.708

0.481

0.65

Ratio organic modifier: mobile phase (Acetonitrile) (v/v)

 40:60

4354.9

1100.3

1.147

4.696

0.475

0.677

 60:40

1925.3

1004.5

1.173

4.667

0.453

0.679

 80:20

7501.7

1648.5

1.187

4.75

0.468

0.647

 90:10

8304.6

2064.8

1.19

4.792

0.458

0.653

Effect of flow rate (mL/min)

 0.3

 

1041

 

8.196

 

0.816

 0.5

 

1090.7

 

4.613

 

0.613

 1

5093.6

 

1.53

 

0.287

 

 1.2

1117.9

 

1.175

 

0.465

 
  1. \( {\text{Number of theoretical plates }}\left( {\text{N}} \right)\, = \,5.54\left( {\frac{{{t}_{{R}} }}{{{Wh}/2}} } \right)^{2} \)
  2. \( {\text{Tailing factor }}\left( {\text{T}} \right)\, = \,\frac{{{W}_{0.5} }}{{2{f}}}{\text{k}^{\prime}} \, = \,{{({\text{tR}} {-}{\text{ t}}0)} \mathord{\left/ {\vphantom {{({\text{tR}} {-}{\text{ t}}0)} {{\text{t}}0}}} \right. \kern-0pt} {{\text{t}}0}} \),