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Table 2 Optimization of the chromatographic conditions for the determination of SFV by Method 2

From: Two novel UPLC methods utilizing two different analytical columns and different detection approaches for the simultaneous analysis of velpatasvir and sofosbuvir: application to their co-formulated tablet

Parameter No. of theoretical plates (N) Capacity factor (k′) Tailing factor (Tf)
Column (A) Column (B) Column (A) Column (B) Column (A) Column (B)
Column temperature °C
 25 °C 4354.9  1014.2 1.147 4.638  0.475 0.629 
 40 °C 2950.3 756.4 1.057 4.404 0.506 0.772
 50 °C 2620.8 677.9 0.989 4.208 0.471 0.73
 65 °C 1895.1 468.9 0.871 3.792 0.468 0.679
pH of mobile phase
 2.5 4354.9 1014.2 1.147 4.638 0.475 0.629
 3.5 3981.2 1089 1.145 4.667 0.526 0.588
 5 4617.2 1066.9 1.145 4.667 0.455 0.572
Type of organic modifier of Conc 40% (v/v)
 Acetonitrile 4390.4 1004.5 1.236 4.667 0.446 0.679
 Methanol 5073.4 1214.5 1.179 4.738 0.474 0.664
 Ethanol 3342 925.4 1.175 4.708 0.481 0.65
Ratio organic modifier: mobile phase (Acetonitrile) (v/v)
 40:60 4354.9 1100.3 1.147 4.696 0.475 0.677
 60:40 1925.3 1004.5 1.173 4.667 0.453 0.679
 80:20 7501.7 1648.5 1.187 4.75 0.468 0.647
 90:10 8304.6 2064.8 1.19 4.792 0.458 0.653
Effect of flow rate (mL/min)
 0.3   1041   8.196   0.816
 0.5   1090.7   4.613   0.613
 1 5093.6   1.53   0.287  
 1.2 1117.9   1.175   0.465  
  1. \( {\text{Number of theoretical plates }}\left( {\text{N}} \right)\, = \,5.54\left( {\frac{{{t}_{{R}} }}{{{Wh}/2}} } \right)^{2} \)
  2. \( {\text{Tailing factor }}\left( {\text{T}} \right)\, = \,\frac{{{W}_{0.5} }}{{2{f}}}{\text{k}^{\prime}} \, = \,{{({\text{tR}} {-}{\text{ t}}0)} \mathord{\left/ {\vphantom {{({\text{tR}} {-}{\text{ t}}0)} {{\text{t}}0}}} \right. \kern-0pt} {{\text{t}}0}} \),