Skip to main content

Table 1 Analytical parameters and validation results of the determination of PAR and IBU by the proposed methods

From: Three smart spectrophotometric methods for resolution of severely overlapped binary mixture of Ibuprofen and Paracetamol in pharmaceutical dosage form

Parameter Ratio difference Constant center Mean centering
PAR IBU PAR IBU PAR IBU
Linearity (μg/mL) 2–20 2–50 2–20 2–50 2–20 2–50
Slope 0.0538 0.1267 0.5544 0.3916 318.22 0.1949
Standard error of the slope 0.00067 0.00084 0.00176 0.00107 3.770 0.000354
Intercept 0.009016 0.1203 0.0318 − 0.0114 108.66 0.5010
Standard error of intercept 0.001722 0.02372 0.01096 0.00091 50.507 0.01177
Standard deviation of residuals from line 0.01033 0.03782 0.01637 0.00109 57.851 0.01122
Accuracy (mean ± SD) 99.72 ± 1.71 100.11 ± 0.53 99.64 ± 0.803 99.97 ± 0.641 100.21 ± 1.24 100.56 ± 0.36
Intraday precision (RSD%)a 0.14 0.42 0.36 0.57 0.44 0.75
Interday precision (RSD%)b 0.57 0.45 0.78 0.64 1.21 1.04
LOD 0.63 0.985 0.097 0.01 0.59 0.189
LOQ 1.537 1.985 0.295 0.0278 1.81 0.575
  1. aIntraday precision: average of 3 different concentrations in triplicate (n = 9) within the same day
  2. bInterday precision:average of 3 different concentrations in triplicate (n = 9) repeated on 3 successive days