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Table 5 [M-H],MS2, MS3, and MS4 data of acylated-di-C-glycosyl flavones, when the position of acylation is set to 6″-O in the sugar in Flickingeria fimbriata

From: Identification of C-glycosyl flavones by high performance liquid chromatography electrospray ionization mass spectrometry and quantification of five main C-glycosyl flavones in Flickingeria fimbriata

Peak No. Rt (min) Compounds Molecular formula [M-H] (m/z) MS2 (m/z) MS3 (m/z) MS4 (m/z)
− 120 − 90 − 60 -Acyla -Acidb -Acylhexosec [Ag-H+84] [Ag-H+114] [Ag-H+56]− [Ag-H+28]
17 54.13 Apig-6-C-(6″-O-fer)-β-d-glu-8-C-β-d-xyl C36H36O17 739 618 (1) 649 (1.6) 679 (4) 563 (100) 545 (12) 443 (1) 353 (13) 383 (35)
16 53.83 Apig-6-C-β-d-glu-8-C-(6″-O-fer)-β-d-glu C37H38O18 769 649 (100) 679 (56) 709 (5) 593 (17) 575 (35) 473 (32) 353 (100) 383 (14) 325 (86) 297 (100)
22 61.74 Apig-6-C-β-d-glu-8-C-(6″-O-3,4-dim)-β-d-glu C38H40O18 783 663 (100) 723 (5) 575 (91) 473 (48) 353 (100) 383 (17) 325 (36) 297 (100)
23 63.60 Apig-6-C-β-d-glu-8-C-(6″-O-3,4,5-trim)-β-d-glu C39H42O19 813 693 (100) 723 (50) 753 (1) 575 (79) 473 (36) 353 (100) 383 (11)
18 56.89 Apig-6-C-α-l-ara-8-C-(6″-O-ρ-coum)β-d-glu C35H34O16 709 619 (100) 649 (54) 563 (9) 545 (12) 443 (11) 353 (100) 383 (36) 325 (30) 297 (100)
20 57.93 Apig-6-C-α-l-ara-8-C-(6″-O-fer)-β-d-glu C36H36O17 739 649 (100) 679 (69) 563 (11) 545 (1.5) 443 (19) 353 (100) 383 (16) 325 (59) 297 (100)
26 65.01 Apig-6-C-β-d-xyl-8-C-(6″-O-3,4-dim)-β-d-glu C37H38O17 753 663 (100) 693 (70) 545 (75) 443 (33) 353 (100) 383 (16) 325 (58) 297 (100)
28 66.97 Apig-6-C-β-d-xyl-8-C-(6″-O-3,4,5-trim)-β-d-glu C38H40O18 783 693 (100) 723 (69) 545 (68) 443 (30) 353 (67) 383 (2) 325 (2)
29 67.24 Apig-6-C-α-l-ara-8-C-(6″-O-3,4-dim)-β-d-glu C37H38O17 753 663 (100) 693 (70) 545 (95) 443 (91) 353 (100) 383 (9) 325 (100) 297 (92)
30 69.04 Apig-6-C-α-l-ara-8-C-(6″-O-3,4,5-trim)-β-d-glu C38H40O18 783 693 (100) 723 (58) 545 (74) 443 (30) 353 (100) 383 (32) 325 (99) 297 (100)
25 64.79 Chrys-6-C-β-d-xyl-8-C-(6″-O-3,4-dim)-β-d-glu C38H40O18 783 693 (79) 723 (49) 575 (72) 473 (100) 383 (100) 413 (44) 355 (37) 327 (8)
27 66.52 Chrys-6-C-β-d-xyl-8-C-(6″-O-3,4,5-trim)-β-d-glu C38H40O18 813 723 (76) 753 (55) 575 (71) 473 (100) 383 (100) 413 (36) 355 (33) 327 (2.4)
  1. Main observed fragments. Other ions were found, but they have not been included
  2. Apig, Apigenin; Chrys, Chrysoeriol; glu, glucoside; ara, arabinoside; xyl, xyloside; ρ-coum, ρ-coumaroyl; fer, feruloyl; 3,4-dim, 3,4-dimethoxycinnamoyl; 3,4,5-trim, 3,4,5-trimethoxycinnamoyl
  3. a-Acyl: ρ-coumaroyl (− 146 Da); feruloyl (− 176 Da)
  4. b-Acid: ρ-coumaric acid (− 164 Da); ferulic acid (− 194 Da); 3,4-dimethoxycinnamoyl acid (− 208 Da); 3,4,5-trimethoxycinnamoyl acid (− 238 Da)
  5. c-Acylhexose: ρ-coumaroylhexose (− 266 Da); feruloylhexose (− 296 Da); 3,4-dimethoxycinnamoylhexose (− 310 Da); 3,4,5-trimethoxycinnamoylhexose (− 340 Da)