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Table 4 Stability of EMPA in human plasma

From: A UPLC/DAD method for simultaneous determination of empagliflozin and three related substances in spiked human plasma

Parameters Concentration (ng/mL) %Recovery %RSD %RE
Short term
At 25 °C, 24 h
50 (LLOQ) 95.37 13.5 − 4.63
100 (LQC) 104.1 10.9 4.10
300 (MQC) 93.71 9.62 − 6.29
500 (HQC) 101.5 8.07 1.50
700 (ULOQ) 99.01 4.39 − 0.99
Long term
At − 20 °C, 30 days
50 (LLOQ) 90.63 11.2 − 9.37
100 (LQC) 93.82 7.37 − 6.18
300 (MQC) 95.13 5.97 − 4.87
500 (HQC) 103.7 7.11 3.68
700 (ULOQ) 108.4 8.94 8.42
Three freeze–thaw cycles thawed at 25 °C for 2 h, refrozen 24 h 50 (LLOQ) 92.07 12.9 − 7.93
100 (LQC) 101.7 8.66 1.70
300 (MQC) 91.91 10.1 − 8.09
500 (HQC) 93.44 9.79 − 6.56
700 (ULOQ) 97.12 4.64 − 2.88