Table 4 Stability of EMPA in human plasma
Parameters | Concentration (ng/mL) | %Recovery | %RSD | %RE |
|---|---|---|---|---|
Short term At 25 °C, 24 h | 50 (LLOQ) | 95.37 | 13.5 | − 4.63 |
100 (LQC) | 104.1 | 10.9 | 4.10 | |
300 (MQC) | 93.71 | 9.62 | − 6.29 | |
500 (HQC) | 101.5 | 8.07 | 1.50 | |
700 (ULOQ) | 99.01 | 4.39 | − 0.99 | |
Long term At − 20 °C, 30 days | 50 (LLOQ) | 90.63 | 11.2 | − 9.37 |
100 (LQC) | 93.82 | 7.37 | − 6.18 | |
300 (MQC) | 95.13 | 5.97 | − 4.87 | |
500 (HQC) | 103.7 | 7.11 | 3.68 | |
700 (ULOQ) | 108.4 | 8.94 | 8.42 | |
Three freeze–thaw cycles thawed at 25 °C for 2 h, refrozen 24 h | 50 (LLOQ) | 92.07 | 12.9 | − 7.93 |
100 (LQC) | 101.7 | 8.66 | 1.70 | |
300 (MQC) | 91.91 | 10.1 | − 8.09 | |
500 (HQC) | 93.44 | 9.79 | − 6.56 | |
700 (ULOQ) | 97.12 | 4.64 | − 2.88 |