Table 3 Recovery and precision of EMPA and its related substances
Compound | Concentration added (ng/mL) | Intra-day% (n = 5) | Inter-day% (n = 5) | ||||
|---|---|---|---|---|---|---|---|
Concentration found (ng/mL) | Recovery % (n = 5) | %RSD | Concentration found (ng/mL) | Recovery % (n = 5) | %RSD | ||
EMPA | 50 (LLOQ) | 46.21 | 92.42 | 13.8 | 47.15 | 94.30 | 11.1 |
100 (LQC) | 98.60 | 98.60 | 4.34 | 96.22 | 96.22 | 6.38 | |
300 (MQC) | 293.79 | 97.93 | 5.90 | 301.7 | 100.6 | 9.45 | |
500 (HQC) | 501.36 | 100.27 | 4.22 | 494.3 | 98.86 | 3.61 | |
700 (ULOQ) | 673.54 | 96.22 | 3.67 | 706.5 | 100.9 | 2.59 | |
Mean | 97.09 | 6.38 | 98.18 | 6.63 | |||
ERS1 | 40 (LLOQ) | 36.19 | 90.48 | 11.9 | 43.71 | 109.3 | 12.4 |
80 (LQC) | 84.48 | 105.6 | 6.76 | 75.98 | 94.98 | 9.11 | |
120 (MQC) | 114.6 | 95.50 | 8.42 | 110.7 | 92.25 | 8.92 | |
160 (HQC) | 154.9 | 96.84 | 3.90 | 156.2 | 97.63 | 4.15 | |
200 (ULOQ) | 201.7 | 100.9 | 5.32 | 206.3 | 103.2 | 5.07 | |
Mean | 97.86 | 7.26 | 99.47 | 7.93 | |||
ERS2 | 40 (LLOQ) | 36.68 | 91.70 | 13.2 | 35.82 | 89.55 | 14.3 |
80 (LQC) | 77.86 | 97.33 | 5.92 | 75.37 | 94.21 | 7.05 | |
120 (MQC) | 125.5 | 104.6 | 7.09 | 118.6 | 98.83 | 2.64 | |
160 (HQC) | 164.6 | 102.9 | 6.39 | 169.4 | 105.9 | 6.08 | |
200 (ULOQ) | 199.7 | 99.85 | 7.11 | 204.9 | 102.5 | 2.94 | |
Mean | 99.28 | 7.94 | 98.20 | 6.60 | |||
ERS3 | 40 (LLOQ) | 37.72 | 94.30 | 14.6 | 38.70 | 96.75 | 12.9 |
80 (LQC) | 81.68 | 102.1 | 9.63 | 79.11 | 98.89 | 10.3 | |
120 (MQC) | 111.7 | 93.08 | 6.37 | 122.9 | 102.4 | 7.25 | |
160 (HQC) | 154.9 | 97.34 | 4.08 | 164.3 | 102.7 | 3.69 | |
200 (ULOQ) | 196.1 | 98.05 | 2.01 | 195.2 | 97.60 | 2.98 | |
Mean | 96.97 | 7.34 | 99.67 | 7.42 | |||