Skip to main content

Table 3 Recovery and precision of EMPA and its related substances

From: A UPLC/DAD method for simultaneous determination of empagliflozin and three related substances in spiked human plasma

Compound Concentration added (ng/mL) Intra-day%
(n = 5)
Inter-day%
(n = 5)
Concentration found (ng/mL) Recovery % (n = 5) %RSD Concentration found (ng/mL) Recovery % (n = 5) %RSD
EMPA 50 (LLOQ) 46.21 92.42 13.8 47.15 94.30 11.1
100 (LQC) 98.60 98.60 4.34 96.22 96.22 6.38
300 (MQC) 293.79 97.93 5.90 301.7 100.6 9.45
500 (HQC) 501.36 100.27 4.22 494.3 98.86 3.61
700 (ULOQ) 673.54 96.22 3.67 706.5 100.9 2.59
Mean    97.09 6.38   98.18 6.63
ERS1 40 (LLOQ) 36.19 90.48 11.9 43.71 109.3 12.4
80 (LQC) 84.48 105.6 6.76 75.98 94.98 9.11
120 (MQC) 114.6 95.50 8.42 110.7 92.25 8.92
160 (HQC) 154.9 96.84 3.90 156.2 97.63 4.15
200 (ULOQ) 201.7 100.9 5.32 206.3 103.2 5.07
Mean    97.86 7.26   99.47 7.93
ERS2 40 (LLOQ) 36.68 91.70 13.2 35.82 89.55 14.3
80 (LQC) 77.86 97.33 5.92 75.37 94.21 7.05
120 (MQC) 125.5 104.6 7.09 118.6 98.83 2.64
160 (HQC) 164.6 102.9 6.39 169.4 105.9 6.08
200 (ULOQ) 199.7 99.85 7.11 204.9 102.5 2.94
Mean    99.28 7.94   98.20 6.60
ERS3 40 (LLOQ) 37.72 94.30 14.6 38.70 96.75 12.9
80 (LQC) 81.68 102.1 9.63 79.11 98.89 10.3
120 (MQC) 111.7 93.08 6.37 122.9 102.4 7.25
160 (HQC) 154.9 97.34 4.08 164.3 102.7 3.69
200 (ULOQ) 196.1 98.05 2.01 195.2 97.60 2.98
Mean    96.97 7.34   99.67 7.42