Fig. 5

Typical chromatogram for the separation of FF (2.4 µg/mL, 2.7 min), GLY (4.5 µg/mL, 5.2 min.) and DEX (IS; 15 μg/mL, 4.4 min) in synthetic mixture using mobile phase. Chromatographic system: Knauer C₁₈ column (150 mm × 4.6 mm with 5.0 µm particle size) Mobile phase mobile phase consisting of acetonitrile: acidified deionized water containing 0.025% SDS pH 3.0 (60: 40% v/v). Flow rate, 1.2 mL/min, UV detection at 210 nm; column temperature, ambient