Skip to main content

Advertisement

Table 3 5-HT stability in spiked medium samples (n = 5)

From: A simple, efficient and rapid HPLC–UV method for the detection of 5-HT in RIN-14B cell extract and cell culture medium

Storage condition Nominal concentration (μg/mL) Mean measured concentration (μg/mL) ± SD 95% confidence intervals Accuracy (%)
Freshly prepared samples 0.3 0.305 ± 0.003 0.301–0.309 101.70
7.5 7.494 ± 0.024 7.463–7.522 99.92
24 h at room temperature 0.3 0.299 ± 0.004 0.295–0.304 99.80
7.5 7.514 ± 0.022 7.487–7.542 100.19
Three freeze–thaw cycles 0.3 0.297 ± 0.003 0.294–0.300 99.03
7.5 7.516 ± 0.011 7.502–7.530 100.21
60 days at 4 ℃ 0.3 0.310 ± 0.001 0.309–0.311 103.03
7.5 7.518 ± 0.004 7.512–7.524 100.24