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Table 1 Effect of different hydrolysis conditions (temperature and time) on recovery of the tested keyhole limpet hemocyanin (KLH) protein sample

From: Determination of immunogenic proteins in biopharmaceuticals by UHPLC–MS amino acid analysis

  Expected concentration [mg mL−1] Determined concentration [mg mL−1] Recovery [%]
110 °C/20 h 20 19.56 97.8
110 °C/24 h 20 19.64 98.2
110 °C/28 h 20 19.14 95.7
120 °C/20 h 20 20.00 100.0
120 °C/24 h 20 20.12 100.6
120 °C/28 h 20 19.84 99.2
130 °C/20 h 20 19.76 98.8
130 °C/24 h 20 20.30 101.5
130 °C/28 h 20 19.74 98.7